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Expression of eosinophil β chain-signaling cytokines receptors, outer-membrane integrins, and type 2 inflammation biomarkers in severe non-allergic eosinophilic asthma.
BMC Pulm Med 2019; 19(1):158BP

Abstract

BACKGROUND

Severe non-allergic eosinophilic asthma (SNEA) is a rare asthma phenotype associated with severe clinical course, frequent exacerbations, and resistance to therapy, including high steroid doses. The key feature is type 2 inflammation with predominant airway eosinophilia. Eosinophil maturation, activation, survivability, and recruitment are mainly induced by interleukin (IL)-3, IL-5 and granulocyte-macrophage colony-stimulating factor (GM-CSF) through their receptors on eosinophil surface and related with integrins activation states. The aim of the study was to estimate the expression of eosinophil β chain-signaling cytokines receptors, outer-membrane integrins, and serum-derived type 2 inflammation biomarkers in SNEA.

METHODS

We examined 8 stable SNEA patients with high inhaled steroid doses, 12 steroid-free patients with non-severe allergic asthma (AA), 12 healthy subjects (HS). Blood eosinophils were isolated using Ficol gradient centrifugation and magnetic separation. Eosinophils were lysed, and mRNA was isolated. Gene expressions of IL-5Rα, IL-3Rα, GM-CSFRα, and α4β1, αMβ2 integrins were analyzed using quantitative real-time reverse transcription polymerase chain reaction. Type 2 inflammation activity was evaluated measuring exhaled nitric oxide concentration (FeNO) collected with the electrochemical sensing device. Serum IL-5, IL-3, GM-CSF, periostin, chemokine ligand (CCL) 17 and eotaxin concentrations were assessed by enzyme-linked immunosorbent assay.

RESULTS

Eosinophils from SNEA patients demonstrated significantly increased gene expression of IL-3Rα, IL-5Rα and GM-CSFRα as well as α4, β1 and αM integrin subunits compared with the AA group. The highest IL-5 serum concentration was in the SNEA group; it significantly differed compared with AA and HS. GM-CSF serum levels were similar in the SNEA and AA groups and were significantly lower in the HS group. No differences in serum IL-3 concentration were found among all groups. Furthermore, serum levels of eotaxin, CCL17 and FeNO, but not periostin, differed in all groups, with the highest levels in SNEA patients.

CONCLUSIONS

Eosinophil demonstrated higher expression of IL-3, IL-5, GM-CSF α-chain receptors and α4, β1, αM integrins subunits in SNEA compared with the AA group. Additionally, SNEA patients had increased serum levels of IL-5, eotaxin and CCL-17.

TRIAL REGISTRATION

ClinicalTrials.gov Identifier NCT03388359.

Authors+Show Affiliations

Department of Pulmonology, Lithuanian University of Health Sciences, Kaunas, Lithuania. virginija.kalinauskaite@lsmuni.lt.Laboratory of Pulmonology, Department of Pulmonology, Lithuanian University of Health Sciences, Kaunas, Lithuania.Laboratory of Pulmonology, Department of Pulmonology, Lithuanian University of Health Sciences, Kaunas, Lithuania.Department of Pulmonology, Lithuanian University of Health Sciences, Kaunas, Lithuania.Department of Pulmonology, Lithuanian University of Health Sciences, Kaunas, Lithuania. Laboratory of Pulmonology, Department of Pulmonology, Lithuanian University of Health Sciences, Kaunas, Lithuania.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31438916

Citation

Kalinauskaite-Zukauske, Virginija, et al. "Expression of Eosinophil Β Chain-signaling Cytokines Receptors, Outer-membrane Integrins, and Type 2 Inflammation Biomarkers in Severe Non-allergic Eosinophilic Asthma." BMC Pulmonary Medicine, vol. 19, no. 1, 2019, p. 158.
Kalinauskaite-Zukauske V, Januskevicius A, Janulaityte I, et al. Expression of eosinophil β chain-signaling cytokines receptors, outer-membrane integrins, and type 2 inflammation biomarkers in severe non-allergic eosinophilic asthma. BMC Pulm Med. 2019;19(1):158.
Kalinauskaite-Zukauske, V., Januskevicius, A., Janulaityte, I., Miliauskas, S., & Malakauskas, K. (2019). Expression of eosinophil β chain-signaling cytokines receptors, outer-membrane integrins, and type 2 inflammation biomarkers in severe non-allergic eosinophilic asthma. BMC Pulmonary Medicine, 19(1), p. 158. doi:10.1186/s12890-019-0904-9.
Kalinauskaite-Zukauske V, et al. Expression of Eosinophil Β Chain-signaling Cytokines Receptors, Outer-membrane Integrins, and Type 2 Inflammation Biomarkers in Severe Non-allergic Eosinophilic Asthma. BMC Pulm Med. 2019 Aug 22;19(1):158. PubMed PMID: 31438916.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Expression of eosinophil β chain-signaling cytokines receptors, outer-membrane integrins, and type 2 inflammation biomarkers in severe non-allergic eosinophilic asthma. AU - Kalinauskaite-Zukauske,Virginija, AU - Januskevicius,Andrius, AU - Janulaityte,Ieva, AU - Miliauskas,Skaidrius, AU - Malakauskas,Kestutis, Y1 - 2019/08/22/ PY - 2018/11/08/received PY - 2019/07/23/accepted PY - 2019/8/24/entrez PY - 2019/8/24/pubmed PY - 2019/8/24/medline KW - CCL17 KW - Eosinophil KW - Eotaxin KW - GM-CSF KW - IL-3 KW - IL-5 KW - Integrins KW - Severe non-allergic eosinophilic asthma SP - 158 EP - 158 JF - BMC pulmonary medicine JO - BMC Pulm Med VL - 19 IS - 1 N2 - BACKGROUND: Severe non-allergic eosinophilic asthma (SNEA) is a rare asthma phenotype associated with severe clinical course, frequent exacerbations, and resistance to therapy, including high steroid doses. The key feature is type 2 inflammation with predominant airway eosinophilia. Eosinophil maturation, activation, survivability, and recruitment are mainly induced by interleukin (IL)-3, IL-5 and granulocyte-macrophage colony-stimulating factor (GM-CSF) through their receptors on eosinophil surface and related with integrins activation states. The aim of the study was to estimate the expression of eosinophil β chain-signaling cytokines receptors, outer-membrane integrins, and serum-derived type 2 inflammation biomarkers in SNEA. METHODS: We examined 8 stable SNEA patients with high inhaled steroid doses, 12 steroid-free patients with non-severe allergic asthma (AA), 12 healthy subjects (HS). Blood eosinophils were isolated using Ficol gradient centrifugation and magnetic separation. Eosinophils were lysed, and mRNA was isolated. Gene expressions of IL-5Rα, IL-3Rα, GM-CSFRα, and α4β1, αMβ2 integrins were analyzed using quantitative real-time reverse transcription polymerase chain reaction. Type 2 inflammation activity was evaluated measuring exhaled nitric oxide concentration (FeNO) collected with the electrochemical sensing device. Serum IL-5, IL-3, GM-CSF, periostin, chemokine ligand (CCL) 17 and eotaxin concentrations were assessed by enzyme-linked immunosorbent assay. RESULTS: Eosinophils from SNEA patients demonstrated significantly increased gene expression of IL-3Rα, IL-5Rα and GM-CSFRα as well as α4, β1 and αM integrin subunits compared with the AA group. The highest IL-5 serum concentration was in the SNEA group; it significantly differed compared with AA and HS. GM-CSF serum levels were similar in the SNEA and AA groups and were significantly lower in the HS group. No differences in serum IL-3 concentration were found among all groups. Furthermore, serum levels of eotaxin, CCL17 and FeNO, but not periostin, differed in all groups, with the highest levels in SNEA patients. CONCLUSIONS: Eosinophil demonstrated higher expression of IL-3, IL-5, GM-CSF α-chain receptors and α4, β1, αM integrins subunits in SNEA compared with the AA group. Additionally, SNEA patients had increased serum levels of IL-5, eotaxin and CCL-17. TRIAL REGISTRATION: ClinicalTrials.gov Identifier NCT03388359. SN - 1471-2466 UR - https://www.unboundmedicine.com/medline/citation/31438916/Expression_of_eosinophil_β_chain-signaling_cytokines_receptors,_outer-membrane_integrins,_and_type_2_inflammation_biomarkers_in_severe_non-allergic_eosinophilic_asthma L2 - https://bmcpulmmed.biomedcentral.com/articles/10.1186/s12890-019-0904-9 DB - PRIME DP - Unbound Medicine ER -