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Two-step regulation of trachealess ensures tight coupling of cell fate with morphogenesis in the Drosophila trachea.
Elife 2019; 8E

Abstract

During organogenesis, inductive signals cause cell differentiation and morphogenesis. However, how these phenomena are coordinated to form functional organs is poorly understood. Here, we show that cell differentiation of the Drosophila trachea is sequentially determined in two steps and that the second step is synchronous with the invagination of the epithelial sheet. The master gene trachealess is dispensable for the initiation of invagination, while it is essential for maintaining the invaginated structure, suggesting that tracheal morphogenesis and differentiation are separately induced. trachealess expression starts in bipotential tracheal/epidermal placode cells. After invagination, its expression is maintained in the invaginated cells but is extinguished in the remaining sheet cells. A trachealess cis-regulatory module that shows both tracheal enhancer activity and silencer activity in the surface epidermal sheet was identified. We propose that the coupling of trachealess expression with the invaginated structure ensures that only invaginated cells canalize robustly into the tracheal fate.

Authors+Show Affiliations

Graduate School of Biostudies, Kyoto University, Kyoto, Japan. The Keihanshin Consortium for Fostering the Next Generation of Global Leaders in Research (K-CONNEX), Kyoto, Japan.Laboratory for Morphogenetic Signaling, RIKEN Center for Biosystems Dynamics Research, Kobe, Japan. Department of Biology, Kobe University Graduate School of Science, Kobe, Japan.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31439126

Citation

Kondo, Takefumi, and Shigeo Hayashi. "Two-step Regulation of Trachealess Ensures Tight Coupling of Cell Fate With Morphogenesis in the Drosophila Trachea." ELife, vol. 8, 2019.
Kondo T, Hayashi S. Two-step regulation of trachealess ensures tight coupling of cell fate with morphogenesis in the Drosophila trachea. Elife. 2019;8.
Kondo, T., & Hayashi, S. (2019). Two-step regulation of trachealess ensures tight coupling of cell fate with morphogenesis in the Drosophila trachea. ELife, 8, doi:10.7554/eLife.45145.
Kondo T, Hayashi S. Two-step Regulation of Trachealess Ensures Tight Coupling of Cell Fate With Morphogenesis in the Drosophila Trachea. Elife. 2019 Aug 23;8 PubMed PMID: 31439126.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Two-step regulation of trachealess ensures tight coupling of cell fate with morphogenesis in the Drosophila trachea. AU - Kondo,Takefumi, AU - Hayashi,Shigeo, Y1 - 2019/08/23/ PY - 2019/01/15/received PY - 2019/07/03/accepted PY - 2019/8/24/entrez PY - 2019/8/24/pubmed PY - 2019/8/24/medline KW - D. melanogaster KW - Drosophila KW - cell differentiation KW - developmental biology KW - epithelial morphogenesis KW - gene expression KW - invagination KW - trachea JF - eLife JO - Elife VL - 8 N2 - During organogenesis, inductive signals cause cell differentiation and morphogenesis. However, how these phenomena are coordinated to form functional organs is poorly understood. Here, we show that cell differentiation of the Drosophila trachea is sequentially determined in two steps and that the second step is synchronous with the invagination of the epithelial sheet. The master gene trachealess is dispensable for the initiation of invagination, while it is essential for maintaining the invaginated structure, suggesting that tracheal morphogenesis and differentiation are separately induced. trachealess expression starts in bipotential tracheal/epidermal placode cells. After invagination, its expression is maintained in the invaginated cells but is extinguished in the remaining sheet cells. A trachealess cis-regulatory module that shows both tracheal enhancer activity and silencer activity in the surface epidermal sheet was identified. We propose that the coupling of trachealess expression with the invaginated structure ensures that only invaginated cells canalize robustly into the tracheal fate. SN - 2050-084X UR - https://www.unboundmedicine.com/medline/citation/31439126/Two-step_regulation_of_trachealess_ensures_tight_coupling_of_cell_fate_with_morphogenesis_in_the_Drosophila_trachea L2 - https://doi.org/10.7554/eLife.45145 DB - PRIME DP - Unbound Medicine ER -
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