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[Comparison of chemical constituents of wild silkworm cocoon and domestic silkworm cocoon by UHPLC-MS technology].
Sheng Wu Gong Cheng Xue Bao. 2019 Aug 25; 35(8):1546-1556.SW

Abstract

Identifying and comparing the chemical constituents of wild silkworm cocoon and silkworm cocoon is of great significance for understanding the domestication of silkworm. In this study, we used high temperature and high pressure and methanol-water system to extract cocoon chemical constituents. We used UHPLC-MS to identify and compare cocoon chemical constituents of wild silkworm and domestic silkworm Dazao and Haoyue strains. The cocoon metabolic fingerprints of wild silkworm and domestic silkworm Dazao and Haoyue strains were obtained by using the UHPLC-MS in the positive ion mode and negative ion mode. By annotation, we found that cocoon chemical compounds with high abundances contained amino acids, flavonoids, alkaloids, terpenes, organic acids, and lignans. PLS-DA showed that the cocoon components were significantly different among the wild silkworm and two domestic silkworm strains Dazao and Haoyue. Proline, leucine/isoleucine and phenylalanine showed significantly higher abundances in the cocoon of domestic silkworm Dazao strain than in those of wild silkworm and domestic silkworm Haoyue strain. The flavonoid secondary metabolites are abundant in the Dazao cocoon, including quercetin, isoquercetin, quercetin 3-O-sophoroside, quercetin-3-O-α-L-rhamnoside, quercetin-3-O- rutinoside, and kaempferol. The other secondary metabolites, alkaloids, terpenes and lignans, showed higher abundances in the wild silkworm cocoon than in the domestic silkworm cocoon, including neurine, candicine, pilocarpidine, artemisiifolin, eupassopin, and eudesobovatol. By exposing cocoons to UV light and observing the green fluorescence of flavonoids, we found that Dazao cocoon had the most flavonoids, and Haoyue cocoon had least flavonoids and wild silkworm cocoon had mediate flavonoids. Alkaloids and organic acids are good anti-insect and antimicrobial agents, which have high abundance in the wild silkworm cocoon and could enhance the defense ability of wild silkworm cocoon. Flavonoids are abundant in the cocoon of domestic silkworm Dazao strain, which the main factors are leading to the yellow-green cocoon of Dazao.

Authors+Show Affiliations

College of Biotechnology, Southwestern University, Chongqing 400715, China. Biological Science Research Center, Southwestern University, Chongqing 400715, China.Biological Science Research Center, Southwestern University, Chongqing 400715, China.Biological Science Research Center, Southwestern University, Chongqing 400715, China.Biological Science Research Center, Southwestern University, Chongqing 400715, China.College of Biotechnology, Southwestern University, Chongqing 400715, China. Biological Science Research Center, Southwestern University, Chongqing 400715, China.Biological Science Research Center, Southwestern University, Chongqing 400715, China.Biological Science Research Center, Southwestern University, Chongqing 400715, China.

Pub Type(s)

Journal Article

Language

chi

PubMed ID

31441626

Citation

Zhang, Yan, et al. "[Comparison of Chemical Constituents of Wild Silkworm Cocoon and Domestic Silkworm Cocoon By UHPLC-MS Technology]." Sheng Wu Gong Cheng Xue Bao = Chinese Journal of Biotechnology, vol. 35, no. 8, 2019, pp. 1546-1556.
Zhang Y, Dong Z, Zhao D, et al. [Comparison of chemical constituents of wild silkworm cocoon and domestic silkworm cocoon by UHPLC-MS technology]. Sheng Wu Gong Cheng Xue Bao. 2019;35(8):1546-1556.
Zhang, Y., Dong, Z., Zhao, D., Li, H., Wang, L., Lin, Y., & Zhao, P. (2019). [Comparison of chemical constituents of wild silkworm cocoon and domestic silkworm cocoon by UHPLC-MS technology]. Sheng Wu Gong Cheng Xue Bao = Chinese Journal of Biotechnology, 35(8), 1546-1556. https://doi.org/10.13345/j.cjb.190027
Zhang Y, et al. [Comparison of Chemical Constituents of Wild Silkworm Cocoon and Domestic Silkworm Cocoon By UHPLC-MS Technology]. Sheng Wu Gong Cheng Xue Bao. 2019 Aug 25;35(8):1546-1556. PubMed PMID: 31441626.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Comparison of chemical constituents of wild silkworm cocoon and domestic silkworm cocoon by UHPLC-MS technology]. AU - Zhang,Yan, AU - Dong,Zhaoming, AU - Zhao,Dongchao, AU - Li,Haoyun, AU - Wang,Lingyan, AU - Lin,Ying, AU - Zhao,Ping, PY - 2019/8/24/entrez PY - 2019/8/24/pubmed PY - 2019/9/11/medline KW - alkaloid KW - cocoon KW - domestic silkworm (Bombyx mori) KW - flavonoid KW - liquid chromatograph-mass spectrometer KW - wild silkworm (Bombyx mandarina) SP - 1546 EP - 1556 JF - Sheng wu gong cheng xue bao = Chinese journal of biotechnology JO - Sheng Wu Gong Cheng Xue Bao VL - 35 IS - 8 N2 - Identifying and comparing the chemical constituents of wild silkworm cocoon and silkworm cocoon is of great significance for understanding the domestication of silkworm. In this study, we used high temperature and high pressure and methanol-water system to extract cocoon chemical constituents. We used UHPLC-MS to identify and compare cocoon chemical constituents of wild silkworm and domestic silkworm Dazao and Haoyue strains. The cocoon metabolic fingerprints of wild silkworm and domestic silkworm Dazao and Haoyue strains were obtained by using the UHPLC-MS in the positive ion mode and negative ion mode. By annotation, we found that cocoon chemical compounds with high abundances contained amino acids, flavonoids, alkaloids, terpenes, organic acids, and lignans. PLS-DA showed that the cocoon components were significantly different among the wild silkworm and two domestic silkworm strains Dazao and Haoyue. Proline, leucine/isoleucine and phenylalanine showed significantly higher abundances in the cocoon of domestic silkworm Dazao strain than in those of wild silkworm and domestic silkworm Haoyue strain. The flavonoid secondary metabolites are abundant in the Dazao cocoon, including quercetin, isoquercetin, quercetin 3-O-sophoroside, quercetin-3-O-α-L-rhamnoside, quercetin-3-O- rutinoside, and kaempferol. The other secondary metabolites, alkaloids, terpenes and lignans, showed higher abundances in the wild silkworm cocoon than in the domestic silkworm cocoon, including neurine, candicine, pilocarpidine, artemisiifolin, eupassopin, and eudesobovatol. By exposing cocoons to UV light and observing the green fluorescence of flavonoids, we found that Dazao cocoon had the most flavonoids, and Haoyue cocoon had least flavonoids and wild silkworm cocoon had mediate flavonoids. Alkaloids and organic acids are good anti-insect and antimicrobial agents, which have high abundance in the wild silkworm cocoon and could enhance the defense ability of wild silkworm cocoon. Flavonoids are abundant in the cocoon of domestic silkworm Dazao strain, which the main factors are leading to the yellow-green cocoon of Dazao. SN - 1872-2075 UR - https://www.unboundmedicine.com/medline/citation/31441626/[Comparison_of_chemical_constituents_of_wild_silkworm_cocoon_and_domestic_silkworm_cocoon_by_UHPLC_MS_technology]_ DB - PRIME DP - Unbound Medicine ER -