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Acute-Phase Proteins of Healthy Horses and Horses Naturally Affected by Colic Syndrome.
J Equine Vet Sci 2019; 80:1-4JE

Abstract

The purpose of this study was to evaluate and compare the serum proteinogram, identifying and quantifying the acute-phase proteins (APPs) of healthy horses and those naturally affected by colic syndrome. Blood samples were collected from 9 healthy horses and 17 horses with clinical (G1) or surgical (G2) colic at the time of arrival at the veterinary hospital (M0) and 24 (M1), 48 (M2), and 72 (M3) hours after the initiation of treatment. The APPs were separated by polyacrylamide gel electrophoresis (SDS-PAGE), and their concentrations were determined by computerized densitometry. Protein identification was performed using matrix assisted laser desorption/ionization-time of flight mass spectrometry. Nine APPs were identified: α2-macroglobulin, ceruloplasmin, transferrin, albumin, α1-antitrypsin, haptoglobin, α1-acid glycoprotein, apolipoprotein A1, and serum amyloid A. Ceruloplasmin (Cp) presented an elevation at M0 in G2 in relation to G1 and the control group (CG). Serum amyloid A (SAA) presented an elevation with a difference at M3 in G1 and G2, compared with CG. In the comparison between the time points within each group (G1 and G2), there was an increase in SAA in G2 with a difference at M1, M2, and M3. Cp and SAA were suitable for evaluating the acute-phase response in horses with colic. Cp was an indicator of colic with surgical resolution. SAA presented favorable behavior for its use as a biomarker to establish the prognosis in horses with colic. The serial measurement of these proteins was useful in differentiating the clinical or surgical treatment and prognosis of horses with this disorder.

Authors+Show Affiliations

Veterinary Departament, Universidade Federal de Viçosa, Viçosa, Minas Gerais, Brazil.Professor of Veterinary Clinical Pathology at Veterinary Departament, Universidade Federal de Viçosa, Viçosa-MG, Brazil. Electronic address: leandroabreu@ufv.br.Veterinary Departament, Universidade Federal de Viçosa, Viçosa, Minas Gerais, Brazil.Veterinary Departament, Universidade Federal de Viçosa, Viçosa, Minas Gerais, Brazil.Universidade Federal do Sul e Sudeste do Pará, Xinguará, Pará, Brazil.Veterinary Departament, Universidade Federal de Viçosa, Viçosa, Minas Gerais, Brazil.Veterinary Departament, Universidade Federal de Viçosa, Viçosa, Minas Gerais, Brazil.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31443825

Citation

Souto, Pollyana Cordeiro, et al. "Acute-Phase Proteins of Healthy Horses and Horses Naturally Affected By Colic Syndrome." Journal of Equine Veterinary Science, vol. 80, 2019, pp. 1-4.
Souto PC, Fonseca LAD, Orozco AMO, et al. Acute-Phase Proteins of Healthy Horses and Horses Naturally Affected by Colic Syndrome. J Equine Vet Sci. 2019;80:1-4.
Souto, P. C., Fonseca, L. A. D., Orozco, A. M. O., Lopez, C. J. R., Ermita, P. A. N., Carvalho Filho, W. P., & Girardi, F. M. (2019). Acute-Phase Proteins of Healthy Horses and Horses Naturally Affected by Colic Syndrome. Journal of Equine Veterinary Science, 80, pp. 1-4. doi:10.1016/j.jevs.2019.06.002.
Souto PC, et al. Acute-Phase Proteins of Healthy Horses and Horses Naturally Affected By Colic Syndrome. J Equine Vet Sci. 2019;80:1-4. PubMed PMID: 31443825.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Acute-Phase Proteins of Healthy Horses and Horses Naturally Affected by Colic Syndrome. AU - Souto,Pollyana Cordeiro, AU - Fonseca,Leandro Abreu da, AU - Orozco,Andres Mauricio Ortega, AU - Lopez,Camilo Jose Ramirez, AU - Ermita,Pedro Ancelmo Nunes, AU - Carvalho Filho,Wilson Pinheiro de, AU - Girardi,Fabricia Modolo, Y1 - 2019/06/19/ PY - 2019/04/25/received PY - 2019/06/06/revised PY - 2019/06/08/accepted PY - 2019/8/25/entrez KW - Acute abdomen KW - Acute-phase response KW - Inflammation KW - Serum amyloid A SP - 1 EP - 4 JF - Journal of equine veterinary science JO - J. Equine Vet. Sci. VL - 80 N2 - The purpose of this study was to evaluate and compare the serum proteinogram, identifying and quantifying the acute-phase proteins (APPs) of healthy horses and those naturally affected by colic syndrome. Blood samples were collected from 9 healthy horses and 17 horses with clinical (G1) or surgical (G2) colic at the time of arrival at the veterinary hospital (M0) and 24 (M1), 48 (M2), and 72 (M3) hours after the initiation of treatment. The APPs were separated by polyacrylamide gel electrophoresis (SDS-PAGE), and their concentrations were determined by computerized densitometry. Protein identification was performed using matrix assisted laser desorption/ionization-time of flight mass spectrometry. Nine APPs were identified: α2-macroglobulin, ceruloplasmin, transferrin, albumin, α1-antitrypsin, haptoglobin, α1-acid glycoprotein, apolipoprotein A1, and serum amyloid A. Ceruloplasmin (Cp) presented an elevation at M0 in G2 in relation to G1 and the control group (CG). Serum amyloid A (SAA) presented an elevation with a difference at M3 in G1 and G2, compared with CG. In the comparison between the time points within each group (G1 and G2), there was an increase in SAA in G2 with a difference at M1, M2, and M3. Cp and SAA were suitable for evaluating the acute-phase response in horses with colic. Cp was an indicator of colic with surgical resolution. SAA presented favorable behavior for its use as a biomarker to establish the prognosis in horses with colic. The serial measurement of these proteins was useful in differentiating the clinical or surgical treatment and prognosis of horses with this disorder. SN - 0737-0806 UR - https://www.unboundmedicine.com/medline/citation/31443825/Acute-Phase_Proteins_of_Healthy_Horses_and_Horses_Naturally_Affected_by_Colic_Syndrome DB - PRIME DP - Unbound Medicine ER -