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Quantification of B-vitamins from different fresh milk samples using ultra-high performance liquid chromatography mass spectrometry/selected reaction monitoring methods.
J Chromatogr A 2019; :460452JC

Abstract

Absolute quantification of B-vitamins in milk is becoming imperative to correlate its impact on child/human health. In today's world of changing food habits and environmental pollution, there is concern if milk is what we think it to be. In the present work, ultra-high performance liquid chromatography mass spectrometry/selected reaction monitoring (UPHPLC-MS/SRM) methods have been developed and validated for quantitative estimation of 21 different B-vitamins [B1-3, B2-3, B3-2, B5-1, B6-5, B8-1, B9-2 vitamins, total choline (betaine, choline and acetylcholine)] from a drop (50 µL) and B12 from 500 µL of milk. These two methods qualify all essential bio-analytical parameters (recovery >80%, accuracy <±15%, variation <±10%) and are highly reproducible. By using these developed methods, B-vitamins in different fresh milk samples from cow, goat, buffalo and pasteurized cow milk (each 10 and total n = 40) were analysed. Total choline is the highest (6.5-10.5 µg/mL) and vitamin B8 is the lowest (16.1-32.9 ng/mL) in all milk samples. Inverse correlation between vitamin B2 and B5 was observed in milk samples. The ratio of vitamin B5/B2 was checked in all milk samples, it is higher in cow (2.64), equal in goat (1.04) and lower in buffalo (0.42) milk. Total B-vitamin content in cow milk is higher (10.5 µg/mL) compared to other three (goat-7.2, buffalo-6.5, pasteurized-8.8 µg/mL). Vitamin B12 is higher in cow milk (3.6 ng/mL) compared to other two fresh milk samples. Different isomers for vitamin B6 were noticed in the fresh milk samples. The complete profile of water soluble vitamins and the ratio of two abundant B vitamins (B5/B2) in milk will be useful to check the nutritional quality and to differentiate the kind of animal milk.

Authors+Show Affiliations

Food Safety & Analytical Quality Control Laboratory, CSIR-Central Food Technological Research Institute, Mysore-570020, India.The Hubert Department of Global Health, Rollins School of Public Health, Emory University, Atlanta, USA.Centre for Health Research and Development, Society for Applied Studies, 45, Kalu Sarai, New Delhi-110016, India.Food Safety & Analytical Quality Control Laboratory, CSIR-Central Food Technological Research Institute, Mysore-570020, India. Electronic address: kannan.rf0829@cftri.res.in.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31443970

Citation

Shetty, Sweekruthi A., et al. "Quantification of B-vitamins From Different Fresh Milk Samples Using Ultra-high Performance Liquid Chromatography Mass Spectrometry/selected Reaction Monitoring Methods." Journal of Chromatography. A, 2019, p. 460452.
Shetty SA, Young MF, Taneja S, et al. Quantification of B-vitamins from different fresh milk samples using ultra-high performance liquid chromatography mass spectrometry/selected reaction monitoring methods. J Chromatogr A. 2019.
Shetty, S. A., Young, M. F., Taneja, S., & Rangiah, K. (2019). Quantification of B-vitamins from different fresh milk samples using ultra-high performance liquid chromatography mass spectrometry/selected reaction monitoring methods. Journal of Chromatography. A, p. 460452. doi:10.1016/j.chroma.2019.460452.
Shetty SA, et al. Quantification of B-vitamins From Different Fresh Milk Samples Using Ultra-high Performance Liquid Chromatography Mass Spectrometry/selected Reaction Monitoring Methods. J Chromatogr A. 2019 Aug 13;460452. PubMed PMID: 31443970.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Quantification of B-vitamins from different fresh milk samples using ultra-high performance liquid chromatography mass spectrometry/selected reaction monitoring methods. AU - Shetty,Sweekruthi A, AU - Young,Melissa F, AU - Taneja,Sunita, AU - Rangiah,Kannan, Y1 - 2019/08/13/ PY - 2019/07/05/received PY - 2019/08/08/revised PY - 2019/08/12/accepted PY - 2019/8/25/entrez KW - B-vitamins KW - Milk KW - Nutrition KW - Quantification KW - UHPLC–MS/SRM method SP - 460452 EP - 460452 JF - Journal of chromatography. A JO - J Chromatogr A N2 - Absolute quantification of B-vitamins in milk is becoming imperative to correlate its impact on child/human health. In today's world of changing food habits and environmental pollution, there is concern if milk is what we think it to be. In the present work, ultra-high performance liquid chromatography mass spectrometry/selected reaction monitoring (UPHPLC-MS/SRM) methods have been developed and validated for quantitative estimation of 21 different B-vitamins [B1-3, B2-3, B3-2, B5-1, B6-5, B8-1, B9-2 vitamins, total choline (betaine, choline and acetylcholine)] from a drop (50 µL) and B12 from 500 µL of milk. These two methods qualify all essential bio-analytical parameters (recovery >80%, accuracy <±15%, variation <±10%) and are highly reproducible. By using these developed methods, B-vitamins in different fresh milk samples from cow, goat, buffalo and pasteurized cow milk (each 10 and total n = 40) were analysed. Total choline is the highest (6.5-10.5 µg/mL) and vitamin B8 is the lowest (16.1-32.9 ng/mL) in all milk samples. Inverse correlation between vitamin B2 and B5 was observed in milk samples. The ratio of vitamin B5/B2 was checked in all milk samples, it is higher in cow (2.64), equal in goat (1.04) and lower in buffalo (0.42) milk. Total B-vitamin content in cow milk is higher (10.5 µg/mL) compared to other three (goat-7.2, buffalo-6.5, pasteurized-8.8 µg/mL). Vitamin B12 is higher in cow milk (3.6 ng/mL) compared to other two fresh milk samples. Different isomers for vitamin B6 were noticed in the fresh milk samples. The complete profile of water soluble vitamins and the ratio of two abundant B vitamins (B5/B2) in milk will be useful to check the nutritional quality and to differentiate the kind of animal milk. SN - 1873-3778 UR - https://www.unboundmedicine.com/medline/citation/31443970/Quantification_of_B-vitamins_from_different_fresh_milk_samples_using_ultra-high_performance_liquid_chromatography_mass_spectrometry/selected_reaction_monitoring_methods L2 - https://linkinghub.elsevier.com/retrieve/pii/S0021-9673(19)30837-4 DB - PRIME DP - Unbound Medicine ER -