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Live imaging the Foreign Body Response reveals how dampening inflammation reduces fibrosis.
J Cell Sci 2019JC

Abstract

Implanting biomaterials in tissues leads to inflammation and a Foreign Body Response (FBR), which can result in rejection. Here we live image the FBR triggered by surgical suture implantation in a translucent zebrafish model and compare with an acute wound response. We observe inflammation extending from the suture margins, and correlating with subsequent avascular and fibrotic encapsulation zones: sutures that induce more inflammation result in increased zones of avascularity and fibrosis. Moreover, we capture macrophages as they fuse to become multinucleate foreign body giant cells (FBGCs) adjacent to the most pro-inflammatory sutures. Genetic and pharmacological dampening of the inflammatory response minimises the FBR (including FBGC generation) and normalises the status of the tissue surrounding these sutures. This model of FBR in adult zebrafish allows us to live image the process and to modulate it in ways that may lead us towards new strategies to ameliorate and circumvent FBR in humans.

Authors+Show Affiliations

School of Biochemistry, Biomedical Sciences Building, University Walk, University of Bristol, Bristol, BS8 1TD, UK.School of Biochemistry, Biomedical Sciences Building, University Walk, University of Bristol, Bristol, BS8 1TD, UK. Department Oral and Maxillofacial Surgery, University Hospitals Bristol, Lower Maudlin Street, Bristol BS1 2LY, UK.Bristol Royal Infirmary, University Hospitals Bristol NHS Trust, Upper Maudlin Street, Bristol BS2 8HW, UK. School of Physiology, Pharmacology and Neuroscience, Biomedical Sciences Building, University Walk, University of Bristol, Bristol, BS8 1TD, UK.Wolfson Bioimaging Facility, Faculty of Biomedical Sciences, University Walk, University of Bristol, Bristol, BS8 1TD, UK.School of Biochemistry, Biomedical Sciences Building, University Walk, University of Bristol, Bristol, BS8 1TD, UK paul.martin@bristol.ac.uk. School of Physiology, Pharmacology and Neuroscience, Biomedical Sciences Building, University Walk, University of Bristol, Bristol, BS8 1TD, UK. School of Medicine, UHW Main Building, Heath Park, University of Cardiff, Cardiff, CF14 4XN, UK.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31444283

Citation

Gurevich, David B., et al. "Live Imaging the Foreign Body Response Reveals How Dampening Inflammation Reduces Fibrosis." Journal of Cell Science, 2019.
Gurevich DB, French KE, Collin JD, et al. Live imaging the Foreign Body Response reveals how dampening inflammation reduces fibrosis. J Cell Sci. 2019.
Gurevich, D. B., French, K. E., Collin, J. D., Cross, S. J., & Martin, P. (2019). Live imaging the Foreign Body Response reveals how dampening inflammation reduces fibrosis. Journal of Cell Science, doi:10.1242/jcs.236075.
Gurevich DB, et al. Live Imaging the Foreign Body Response Reveals How Dampening Inflammation Reduces Fibrosis. J Cell Sci. 2019 Aug 23; PubMed PMID: 31444283.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Live imaging the Foreign Body Response reveals how dampening inflammation reduces fibrosis. AU - Gurevich,David B, AU - French,Kathryn E, AU - Collin,John D, AU - Cross,Stephen J, AU - Martin,Paul, Y1 - 2019/08/23/ PY - 2019/07/08/received PY - 2019/07/30/accepted PY - 2019/8/25/entrez KW - Angiogenesis KW - Fibrosis KW - Foreign Body KW - Inflammation KW - Wound KW - Zebrafish JF - Journal of cell science JO - J. Cell. Sci. N2 - Implanting biomaterials in tissues leads to inflammation and a Foreign Body Response (FBR), which can result in rejection. Here we live image the FBR triggered by surgical suture implantation in a translucent zebrafish model and compare with an acute wound response. We observe inflammation extending from the suture margins, and correlating with subsequent avascular and fibrotic encapsulation zones: sutures that induce more inflammation result in increased zones of avascularity and fibrosis. Moreover, we capture macrophages as they fuse to become multinucleate foreign body giant cells (FBGCs) adjacent to the most pro-inflammatory sutures. Genetic and pharmacological dampening of the inflammatory response minimises the FBR (including FBGC generation) and normalises the status of the tissue surrounding these sutures. This model of FBR in adult zebrafish allows us to live image the process and to modulate it in ways that may lead us towards new strategies to ameliorate and circumvent FBR in humans. SN - 1477-9137 UR - https://www.unboundmedicine.com/medline/citation/31444283/Live_imaging_the_Foreign_Body_Response_reveals_how_dampening_inflammation_reduces_fibrosis L2 - http://jcs.biologists.org/cgi/pmidlookup?view=long&pmid=31444283 DB - PRIME DP - Unbound Medicine ER -