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Protective effects of betulinic acid on intestinal mucosal injury induced by cyclophosphamide in mice.
Pharmacol Rep 2019; 71(5):929-939PR

Abstract

BACKGROUND

Betulinic acid (BA) is a plant-derived pentacyclic triterpenoid with a variety of biological activities. The purpose of this study was to assess the potential protective role of BA against intestinal mucosal injury induced by cyclophosphamide (CYP) treatment.

METHODS

Mice were pretreated with BA daily (0.05, 0.5, and 5.0 mg/kg) for 14 days, then injected intraperitoneally with CYP (50 mg/kg) for 2 days.

RESULTS

BA pretreatment reduced the contents of malondialdehyde (MDA) and glutathione (GSH), decreased the activity of superoxide dismutase (SOD) in small intestine, increased villus hight/crypt depth ratio and restored the morphology of intestinal villi in CYP-induced mice. Moreover, BA pretreatment could significantly down-regulate the levels of pro-inflammatory cytokines interleukin-5 (IL-5), IL-17, IL-12(P70) and tumor necrosis factor α (TNF-α), reduced production of chemokines macrophage inflammatory protein-1α (MIP-1α), macrophage inflammatory protein-1β (MIP-1β) and regulated upon activation, normal T-cell expressed and secreted (RANTES), and enhanced the levels of anti-inflammatory such as IL-2 and IL-10 in serum, and decreased the mRNA expressions of IL-1β and TNF-α in intestine of CYP-induced mice. Furthermore, RT-PCR demonstrated that BA improved intestinal physical and immunological barrier in CYP-stimulated mice by enhancing the mRNA expressions of zonula occluden 1 (ZO-1) and Claudin-1.

CONCLUSIONS

BA might be considered as an effective agent in the amelioration of the intestinal mucosal resulting from CYP treatment.

Authors+Show Affiliations

Colleges of Veterinary Medicine, Hunan Agricultural University, Changsha City, China.Colleges of Veterinary Medicine, Hunan Agricultural University, Changsha City, China.Colleges of Veterinary Medicine, Hunan Agricultural University, Changsha City, China.Colleges of Veterinary Medicine, Hunan Agricultural University, Changsha City, China.Colleges of Veterinary Medicine, Hunan Agricultural University, Changsha City, China.Colleges of Veterinary Medicine, Hunan Agricultural University, Changsha City, China.Colleges of Veterinary Medicine, Hunan Agricultural University, Changsha City, China.Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, Wrocław University of Environmental and Life Sciences, Wrocław, Poland.Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, Wrocław University of Environmental and Life Sciences, Wrocław, Poland.Colleges of Veterinary Medicine, Hunan Agricultural University, Changsha City, China. Electronic address: 12145090@qq.com.Colleges of Veterinary Medicine, Hunan Agricultural University, Changsha City, China. Electronic address: yijine@gmail.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31450028

Citation

Wang, Xihong, et al. "Protective Effects of Betulinic Acid On Intestinal Mucosal Injury Induced By Cyclophosphamide in Mice." Pharmacological Reports : PR, vol. 71, no. 5, 2019, pp. 929-939.
Wang X, Yuan Z, Zhu L, et al. Protective effects of betulinic acid on intestinal mucosal injury induced by cyclophosphamide in mice. Pharmacol Rep. 2019;71(5):929-939.
Wang, X., Yuan, Z., Zhu, L., Yi, X., Ou, Z., Li, R., ... Yi, J. (2019). Protective effects of betulinic acid on intestinal mucosal injury induced by cyclophosphamide in mice. Pharmacological Reports : PR, 71(5), pp. 929-939. doi:10.1016/j.pharep.2019.05.004.
Wang X, et al. Protective Effects of Betulinic Acid On Intestinal Mucosal Injury Induced By Cyclophosphamide in Mice. Pharmacol Rep. 2019 May 7;71(5):929-939. PubMed PMID: 31450028.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Protective effects of betulinic acid on intestinal mucosal injury induced by cyclophosphamide in mice. AU - Wang,Xihong, AU - Yuan,Zhihang, AU - Zhu,Lijuan, AU - Yi,Xianglian, AU - Ou,Zhaoping, AU - Li,Rongfang, AU - Tan,Zhuliang, AU - Pozniak,Blazej, AU - Obminska-Mrukowicz,Bozena, AU - Wu,Jing, AU - Yi,Jine, Y1 - 2019/05/07/ PY - 2018/10/24/received PY - 2019/04/21/revised PY - 2019/05/06/accepted PY - 2019/8/27/pubmed PY - 2019/8/27/medline PY - 2019/8/27/entrez KW - Betulinic acid KW - Cyclophosphamide KW - Intestinal mucosal injury KW - Protection SP - 929 EP - 939 JF - Pharmacological reports : PR JO - Pharmacol Rep VL - 71 IS - 5 N2 - BACKGROUND: Betulinic acid (BA) is a plant-derived pentacyclic triterpenoid with a variety of biological activities. The purpose of this study was to assess the potential protective role of BA against intestinal mucosal injury induced by cyclophosphamide (CYP) treatment. METHODS: Mice were pretreated with BA daily (0.05, 0.5, and 5.0 mg/kg) for 14 days, then injected intraperitoneally with CYP (50 mg/kg) for 2 days. RESULTS: BA pretreatment reduced the contents of malondialdehyde (MDA) and glutathione (GSH), decreased the activity of superoxide dismutase (SOD) in small intestine, increased villus hight/crypt depth ratio and restored the morphology of intestinal villi in CYP-induced mice. Moreover, BA pretreatment could significantly down-regulate the levels of pro-inflammatory cytokines interleukin-5 (IL-5), IL-17, IL-12(P70) and tumor necrosis factor α (TNF-α), reduced production of chemokines macrophage inflammatory protein-1α (MIP-1α), macrophage inflammatory protein-1β (MIP-1β) and regulated upon activation, normal T-cell expressed and secreted (RANTES), and enhanced the levels of anti-inflammatory such as IL-2 and IL-10 in serum, and decreased the mRNA expressions of IL-1β and TNF-α in intestine of CYP-induced mice. Furthermore, RT-PCR demonstrated that BA improved intestinal physical and immunological barrier in CYP-stimulated mice by enhancing the mRNA expressions of zonula occluden 1 (ZO-1) and Claudin-1. CONCLUSIONS: BA might be considered as an effective agent in the amelioration of the intestinal mucosal resulting from CYP treatment. SN - 1734-1140 UR - https://www.unboundmedicine.com/medline/citation/31450028/Protective_effects_of_betulinic_acid_on_intestinal_mucosal_injury_induced_by_cyclophosphamide_in_mice L2 - https://linkinghub.elsevier.com/retrieve/pii/S1734-1140(18)30618-2 DB - PRIME DP - Unbound Medicine ER -