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Microbiological analysis of endotracheal aspirate and endotracheal tube cultures in mechanically ventilated patients.
BMC Pulm Med 2019; 19(1):162BP

Abstract

BACKGROUND

To compare the microbiological culture within endotracheal aspirate specimens (ETAs) and endotracheal tube specimens (ETTs) in patients undergoing mechanical ventilation (MV) by statistical tools.

METHOD

ETAs and ETTs from a total number of 81 patients, who were undergoing MV at the intensive care unit (ICU) of Jiading Central Hospital Affiliated Shanghai University of Medicine & Health Sciences from September 1st, 2017 to August 31st, 2018, were collected for microbiological culture analysis. Correlation of ETAs and ETTs cultures were obtained by Spear-men correlation analysis, while the consistency of the two specimens was determined by Kappa analysis and principal component analysis (PCA).

RESULTS

Microbiological culture from both ETAs and ETTs showed that Acinetobacter baumannii, Pseudomonas aeruginosa, Staphylococcus aureus, and Klebsiella pneumoniae were the main pathogens, with Spear-man correlation coefficients of 0.676, 0.951, 0.730 and 0.687 respectively (all P < 0.01), and the overall Spear-man correlation coefficient is 0.757 (P < 0.01). This result shows that two samples were positively correlated. Kappa analysis also revealed high consistency of the microbial culture results from the ETAs and the ETTs (overall κ = 0.751, P < 0.01). The κ values for the four bacteria detected were 0.670, 0.949, 0.723, and 0.687, respectively (all P < 0.001). PCA also revealed high similarity.

CONCLUSION

Combining microbiological culture and statistical analysis of samples collected from 81 patients who were undergoing MV in ICU, we showed that microbe found in the ETAs had high similarity with that found in the ETTs which collected at the end of the catheters. In clinical practice, ETAs analysis is easily accessible meanwhile provides a valuable information for MV patients.

Authors+Show Affiliations

Department of Clinical Laboratory, Jiading District Central Hospital Affiliated Shanghai University of Medicine & Health Sciences, No.1, Chengbei Rd, Jiading District, Shanghai, 201800, China.Department of Critical Care Medicine, Jiading District Central Hospital Affiliated Shanghai University of Medicine & Health Sciences, Shanghai, 201800, China.Shanghai Key Laboratory for Molecular Imaging, Shanghai University of Medicine & Health Sciences, Shanghai, 301318, China.Department of Clinical Laboratory, Jiading District Central Hospital Affiliated Shanghai University of Medicine & Health Sciences, No.1, Chengbei Rd, Jiading District, Shanghai, 201800, China.Department of Critical Care Medicine, Jiading District Central Hospital Affiliated Shanghai University of Medicine & Health Sciences, Shanghai, 201800, China.Department of Pharmacology, Zhejiang University, School of Basic Medical Sciences, Hangzhou, 310058, China.Department of Clinical Laboratory, Jiading District Central Hospital Affiliated Shanghai University of Medicine & Health Sciences, No.1, Chengbei Rd, Jiading District, Shanghai, 201800, China.Department of Critical Care Medicine, Jiading District Central Hospital Affiliated Shanghai University of Medicine & Health Sciences, Shanghai, 201800, China.Department of Critical Care Medicine, Jiading District Central Hospital Affiliated Shanghai University of Medicine & Health Sciences, Shanghai, 201800, China.Emergency Department, Jiading District Central Hospital Affiliated Shanghai University of Medicine & Health Sciences, Shanghai, 201800, China.Shanghai Key Laboratory for Molecular Imaging, Shanghai University of Medicine & Health Sciences, Shanghai, 301318, China.Department of Clinical Laboratory, Jiading District Central Hospital Affiliated Shanghai University of Medicine & Health Sciences, No.1, Chengbei Rd, Jiading District, Shanghai, 201800, China. jzx117@126.com.Clinical Research Center, Jiading District Central Hospital Affiliated Shanghai University of Medicine & Health Sciences, No.1, Chengbei Rd, Jiading District, Shanghai, 201800, China. robie0510@hotmail.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31455270

Citation

Shen, Lijuan, et al. "Microbiological Analysis of Endotracheal Aspirate and Endotracheal Tube Cultures in Mechanically Ventilated Patients." BMC Pulmonary Medicine, vol. 19, no. 1, 2019, p. 162.
Shen L, Wang F, Shi J, et al. Microbiological analysis of endotracheal aspirate and endotracheal tube cultures in mechanically ventilated patients. BMC Pulm Med. 2019;19(1):162.
Shen, L., Wang, F., Shi, J., Xu, W., Jiang, T., Tang, H., ... Chang, Q. (2019). Microbiological analysis of endotracheal aspirate and endotracheal tube cultures in mechanically ventilated patients. BMC Pulmonary Medicine, 19(1), p. 162. doi:10.1186/s12890-019-0926-3.
Shen L, et al. Microbiological Analysis of Endotracheal Aspirate and Endotracheal Tube Cultures in Mechanically Ventilated Patients. BMC Pulm Med. 2019 Aug 27;19(1):162. PubMed PMID: 31455270.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Microbiological analysis of endotracheal aspirate and endotracheal tube cultures in mechanically ventilated patients. AU - Shen,Lijuan, AU - Wang,Fei, AU - Shi,Junfeng, AU - Xu,Weixin, AU - Jiang,Tingting, AU - Tang,Huifang, AU - Yu,Xiuwen, AU - Yin,Hao, AU - Hu,Shanyou, AU - Wu,Xiao, AU - Chan,Siu Kit, AU - Sun,Jie, AU - Chang,Qing, Y1 - 2019/08/27/ PY - 2018/11/30/received PY - 2019/08/19/accepted PY - 2019/8/29/entrez PY - 2019/8/29/pubmed PY - 2019/8/29/medline KW - Clinical value KW - Consistency analysis KW - Endotracheal tube KW - Eracheal aspirate KW - Mechanical ventilation KW - Microorganism SP - 162 EP - 162 JF - BMC pulmonary medicine JO - BMC Pulm Med VL - 19 IS - 1 N2 - BACKGROUND: To compare the microbiological culture within endotracheal aspirate specimens (ETAs) and endotracheal tube specimens (ETTs) in patients undergoing mechanical ventilation (MV) by statistical tools. METHOD: ETAs and ETTs from a total number of 81 patients, who were undergoing MV at the intensive care unit (ICU) of Jiading Central Hospital Affiliated Shanghai University of Medicine & Health Sciences from September 1st, 2017 to August 31st, 2018, were collected for microbiological culture analysis. Correlation of ETAs and ETTs cultures were obtained by Spear-men correlation analysis, while the consistency of the two specimens was determined by Kappa analysis and principal component analysis (PCA). RESULTS: Microbiological culture from both ETAs and ETTs showed that Acinetobacter baumannii, Pseudomonas aeruginosa, Staphylococcus aureus, and Klebsiella pneumoniae were the main pathogens, with Spear-man correlation coefficients of 0.676, 0.951, 0.730 and 0.687 respectively (all P < 0.01), and the overall Spear-man correlation coefficient is 0.757 (P < 0.01). This result shows that two samples were positively correlated. Kappa analysis also revealed high consistency of the microbial culture results from the ETAs and the ETTs (overall κ = 0.751, P < 0.01). The κ values for the four bacteria detected were 0.670, 0.949, 0.723, and 0.687, respectively (all P < 0.001). PCA also revealed high similarity. CONCLUSION: Combining microbiological culture and statistical analysis of samples collected from 81 patients who were undergoing MV in ICU, we showed that microbe found in the ETAs had high similarity with that found in the ETTs which collected at the end of the catheters. In clinical practice, ETAs analysis is easily accessible meanwhile provides a valuable information for MV patients. SN - 1471-2466 UR - https://www.unboundmedicine.com/medline/citation/31455270/Microbiological_analysis_of_endotracheal_aspirate_and_endotracheal_tube_cultures_in_mechanically_ventilated_patients L2 - https://bmcpulmmed.biomedcentral.com/articles/10.1186/s12890-019-0926-3 DB - PRIME DP - Unbound Medicine ER -