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Non-Contact Universal Sample Presentation for Room Temperature Macromolecular Crystallography Using Acoustic Levitation.
Sci Rep 2019; 9(1):12431SR

Abstract

Macromolecular Crystallography is a powerful and valuable technique to assess protein structures. Samples are commonly cryogenically cooled to minimise radiation damage effects from the X-ray beam, but low temperatures hinder normal protein functions and this procedure can introduce structural artefacts. Previous experiments utilising acoustic levitation for beamline science have focused on Langevin horns which deliver significant power to the confined droplet and are complex to set up accurately. In this work, the low power, portable TinyLev acoustic levitation system is used in combination with an approach to dispense and contain droplets, free of physical sample support to aid protein crystallography experiments. This method facilitates efficient X-ray data acquisition in ambient conditions compatible with dynamic studies. Levitated samples remain free of interference from fixed sample mounts, receive negligible heating, do not suffer significant evaporation and since the system occupies a small volume, can be readily installed at other light sources.

Authors+Show Affiliations

School of Science and Technology, Nottingham Trent University, Nottingham, NG11 8NS, UK. rob.morris@ntu.ac.uk.School of Science and Technology, Nottingham Trent University, Nottingham, NG11 8NS, UK.Diamond Light Source, Harwell Science and Innovation Campus, Oxfordshire, OX11 0DE, UK.School of Science and Technology, Nottingham Trent University, Nottingham, NG11 8NS, UK.Diamond Light Source, Harwell Science and Innovation Campus, Oxfordshire, OX11 0DE, UK.Diamond Light Source, Harwell Science and Innovation Campus, Oxfordshire, OX11 0DE, UK.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31455801

Citation

Morris, R H., et al. "Non-Contact Universal Sample Presentation for Room Temperature Macromolecular Crystallography Using Acoustic Levitation." Scientific Reports, vol. 9, no. 1, 2019, p. 12431.
Morris RH, Dye ER, Axford D, et al. Non-Contact Universal Sample Presentation for Room Temperature Macromolecular Crystallography Using Acoustic Levitation. Sci Rep. 2019;9(1):12431.
Morris, R. H., Dye, E. R., Axford, D., Newton, M. I., Beale, J. H., & Docker, P. T. (2019). Non-Contact Universal Sample Presentation for Room Temperature Macromolecular Crystallography Using Acoustic Levitation. Scientific Reports, 9(1), p. 12431. doi:10.1038/s41598-019-48612-4.
Morris RH, et al. Non-Contact Universal Sample Presentation for Room Temperature Macromolecular Crystallography Using Acoustic Levitation. Sci Rep. 2019 Aug 27;9(1):12431. PubMed PMID: 31455801.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Non-Contact Universal Sample Presentation for Room Temperature Macromolecular Crystallography Using Acoustic Levitation. AU - Morris,R H, AU - Dye,E R, AU - Axford,D, AU - Newton,M I, AU - Beale,J H, AU - Docker,P T, Y1 - 2019/08/27/ PY - 2019/04/08/received PY - 2019/07/24/accepted PY - 2019/8/29/entrez PY - 2019/8/29/pubmed PY - 2019/8/29/medline SP - 12431 EP - 12431 JF - Scientific reports JO - Sci Rep VL - 9 IS - 1 N2 - Macromolecular Crystallography is a powerful and valuable technique to assess protein structures. Samples are commonly cryogenically cooled to minimise radiation damage effects from the X-ray beam, but low temperatures hinder normal protein functions and this procedure can introduce structural artefacts. Previous experiments utilising acoustic levitation for beamline science have focused on Langevin horns which deliver significant power to the confined droplet and are complex to set up accurately. In this work, the low power, portable TinyLev acoustic levitation system is used in combination with an approach to dispense and contain droplets, free of physical sample support to aid protein crystallography experiments. This method facilitates efficient X-ray data acquisition in ambient conditions compatible with dynamic studies. Levitated samples remain free of interference from fixed sample mounts, receive negligible heating, do not suffer significant evaporation and since the system occupies a small volume, can be readily installed at other light sources. SN - 2045-2322 UR - https://www.unboundmedicine.com/medline/citation/31455801/Non-Contact_Universal_Sample_Presentation_for_Room_Temperature_Macromolecular_Crystallography_Using_Acoustic_Levitation L2 - http://dx.doi.org/10.1038/s41598-019-48612-4 DB - PRIME DP - Unbound Medicine ER -