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Comparative study on antidiabetic, cytotoxicity, antioxidant and antibacterial properties of biosynthesized silver nanoparticles using outer peels of two varieties of Ipomoea batatas (L.) Lam.
Int J Nanomedicine. 2019; 14:4741-4754.IJ

Abstract

Background

Ipomoea batatas (L.) Lam.(Ib) has high content of various beneficial nutrients which helps in improving and maintaining human health. It is well known as a functional food and also a valuable source of unique natural products. It contains various phenolic and flavonoid bioactive compounds.

Methods

In this study, using the outer peel of two varieties of Ib : Korean red skin sweet potato and Korean pumpkin sweet potato, silver nanoparticles (AgNPs) were synthesized (termed Ib1-AgNps and Ib2-AgNps), respectively. Characterization of Ib1-AgNPs and Ib2-AgNPs was carried out through scanning electron microscopy, Fourier-transform infrared (FT-IR) spectroscopy, energy-dispersive X-ray analysis, X-ray powder diffraction and UV-Vis spectroscopy. Further, the bio-potential of the synthesized AgNPs was investigated by antidiabetic (α-glucosidase assay), antioxidant (free radical scavenging assays), antibacterial (disc diffusion method) and cytotoxicity assays (cell viability against HepG2 cells).

Results

FT-IR spectroscopy revealed the contribution of bioactive compounds existing in Ib1 and Ib2 extracts, in the biosynthesis and equilibrium of the AgNPs. Although the Ib2-AgNPs had a higher atomic percentage of Ag in comparison with Ib1-AgNPs, in the antidiabetic assay, the inhibition percentage of α-glucosidase was higher for AgNPs of Ib1 than Ib2, at all three concentrations examined. From the cytotoxicity results, HepG2 cancer cells were more sensitive to the Ib1-AgNPs in comparison to the Ib2-AgNPs-treated HepG2 cells. The antioxidant prospective was higher in Ib2-AgNPs than Ib1-AgNPs. Moreover, the Ib2-AgNPs showed inhibitory action against all five tested pathogenic bacteria, producing an inhibition zone of 8.74-11.52 mm while Ib1-AgNPs had an inhibitory effect on four of them, with an 8.67-11.23 (mm) inhibition zone.

Conclusions

Overall, the results concluded that the Ib2-AgNPs exhibited relatively higher functional activity than Ib1-AgNPs, which might be credited to the greater abundance of bioactive compounds existing in Ib2 extract that acted as reducing as well as capping agents in the synthesis of Ib2-AgNPs. Overall, the current study highlights a novel cost-effective and eco-friendly AgNPs synthesis using food waste peels with biocompatibility and could be potentially utilized in biomedical and pharmaceutical industries.

Authors+Show Affiliations

Research Institute of Biotechnology & Medical Converged Science, Dongguk University, Seoul, Gyeonggi-do 10326, Republic of Korea.Research Institute of Biotechnology & Medical Converged Science, Dongguk University, Seoul, Gyeonggi-do 10326, Republic of Korea.Department of Biotechnology, Yeungnam University, Gyeongsan, Republic of Korea.Centre for Ayurveda Biology and Holistic Nutrition, The University of Trans-Disciplinary Health Sciences and Technology (TDU), Bengaluru 560064, India.Department of Food Science and Biotechnology, Dongguk University, Seoul, Gyeonggi-do 10326, Republic of Korea.

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

31456635

Citation

Das, Gitishree, et al. "Comparative Study On Antidiabetic, Cytotoxicity, Antioxidant and Antibacterial Properties of Biosynthesized Silver Nanoparticles Using Outer Peels of Two Varieties of Ipomoea Batatas (L.) Lam." International Journal of Nanomedicine, vol. 14, 2019, pp. 4741-4754.
Das G, Patra JK, Basavegowda N, et al. Comparative study on antidiabetic, cytotoxicity, antioxidant and antibacterial properties of biosynthesized silver nanoparticles using outer peels of two varieties of Ipomoea batatas (L.) Lam. Int J Nanomedicine. 2019;14:4741-4754.
Das, G., Patra, J. K., Basavegowda, N., Vishnuprasad, C. N., & Shin, H. S. (2019). Comparative study on antidiabetic, cytotoxicity, antioxidant and antibacterial properties of biosynthesized silver nanoparticles using outer peels of two varieties of Ipomoea batatas (L.) Lam. International Journal of Nanomedicine, 14, 4741-4754. https://doi.org/10.2147/IJN.S210517
Das G, et al. Comparative Study On Antidiabetic, Cytotoxicity, Antioxidant and Antibacterial Properties of Biosynthesized Silver Nanoparticles Using Outer Peels of Two Varieties of Ipomoea Batatas (L.) Lam. Int J Nanomedicine. 2019;14:4741-4754. PubMed PMID: 31456635.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Comparative study on antidiabetic, cytotoxicity, antioxidant and antibacterial properties of biosynthesized silver nanoparticles using outer peels of two varieties of Ipomoea batatas (L.) Lam. AU - Das,Gitishree, AU - Patra,Jayanta Kumar, AU - Basavegowda,Nagaraj, AU - Vishnuprasad,Chethala N, AU - Shin,Han-Seung, Y1 - 2019/07/02/ PY - 2019/03/31/received PY - 2019/05/06/accepted PY - 2019/8/29/entrez PY - 2019/8/29/pubmed PY - 2019/9/17/medline KW - Ipomoea batatas KW - antibacterial KW - antidiabetic KW - antioxidant KW - cytotoxicity KW - silver nanoparticles SP - 4741 EP - 4754 JF - International journal of nanomedicine JO - Int J Nanomedicine VL - 14 N2 - Background: Ipomoea batatas (L.) Lam.(Ib) has high content of various beneficial nutrients which helps in improving and maintaining human health. It is well known as a functional food and also a valuable source of unique natural products. It contains various phenolic and flavonoid bioactive compounds. Methods: In this study, using the outer peel of two varieties of Ib : Korean red skin sweet potato and Korean pumpkin sweet potato, silver nanoparticles (AgNPs) were synthesized (termed Ib1-AgNps and Ib2-AgNps), respectively. Characterization of Ib1-AgNPs and Ib2-AgNPs was carried out through scanning electron microscopy, Fourier-transform infrared (FT-IR) spectroscopy, energy-dispersive X-ray analysis, X-ray powder diffraction and UV-Vis spectroscopy. Further, the bio-potential of the synthesized AgNPs was investigated by antidiabetic (α-glucosidase assay), antioxidant (free radical scavenging assays), antibacterial (disc diffusion method) and cytotoxicity assays (cell viability against HepG2 cells). Results: FT-IR spectroscopy revealed the contribution of bioactive compounds existing in Ib1 and Ib2 extracts, in the biosynthesis and equilibrium of the AgNPs. Although the Ib2-AgNPs had a higher atomic percentage of Ag in comparison with Ib1-AgNPs, in the antidiabetic assay, the inhibition percentage of α-glucosidase was higher for AgNPs of Ib1 than Ib2, at all three concentrations examined. From the cytotoxicity results, HepG2 cancer cells were more sensitive to the Ib1-AgNPs in comparison to the Ib2-AgNPs-treated HepG2 cells. The antioxidant prospective was higher in Ib2-AgNPs than Ib1-AgNPs. Moreover, the Ib2-AgNPs showed inhibitory action against all five tested pathogenic bacteria, producing an inhibition zone of 8.74-11.52 mm while Ib1-AgNPs had an inhibitory effect on four of them, with an 8.67-11.23 (mm) inhibition zone. Conclusions: Overall, the results concluded that the Ib2-AgNPs exhibited relatively higher functional activity than Ib1-AgNPs, which might be credited to the greater abundance of bioactive compounds existing in Ib2 extract that acted as reducing as well as capping agents in the synthesis of Ib2-AgNPs. Overall, the current study highlights a novel cost-effective and eco-friendly AgNPs synthesis using food waste peels with biocompatibility and could be potentially utilized in biomedical and pharmaceutical industries. SN - 1178-2013 UR - https://www.unboundmedicine.com/medline/citation/31456635/Comparative_study_on_antidiabetic_cytotoxicity_antioxidant_and_antibacterial_properties_of_biosynthesized_silver_nanoparticles_using_outer_peels_of_two_varieties_of_Ipomoea_batatas__L___Lam_ L2 - https://dx.doi.org/10.2147/IJN.S210517 DB - PRIME DP - Unbound Medicine ER -