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Fabrication of Three-Dimensional Scaffolds Based on Nano-biomimetic Collagen Hybrid Constructs for Skin Tissue Engineering.
ACS Omega. 2018 Aug 31; 3(8):8605-8611.AO

Abstract

Three-dimensional (3D) biodegradable and biomimetic porous scaffolds are ideal frameworks for skin tissue engineering. In this study, hybrid constructs of 3D scaffolds were successfully fabricated by the freeze-drying method from combinations of the type I collagen (Col) and synthetic poly(lactic acid) (PLLA) or polycaprolactone (PCL). Four different groups of 3D porous scaffolds including PCL, PCL-Col, PCL-PLLA, and PCL-PLLA-Col were fabricated and systematically characterized by hydrogen nuclear magnetic resonance, Fourier transform infrared spectroscopy, and scanning electron microscopy (SEM). Adipose tissue-derived mesenchymal stem cells (AT-MSCs) were seeded in all scaffolds, and the viability, proliferation, and adhesion of the cells were investigated using dimethylthiazol diphenyltetrazolium bromide assay and SEM. The results showed that scaffolds containing Col, particularly PCL-PLLA-Col scaffold, with pore sizes close to 400 nm and being sufficiently interconnected, have significantly greater potential (p < 0.01) for encouraging AT-MSCs adhesion and growth. The PCL-PLLA provided a mechanically stronger mesh support, and the type I Col microsponges encouraged excellent cell adhesion and tissue formation. The scaffold with the best properties could be an appropriate functional candidate for the preparation of artificial skin constructs.

Authors+Show Affiliations

Department of Tissue Engineering, Faculty of Advanced Medical Sciences, Department of Nanotechnology, Faculty of Advanced Medical Sciences, Department of Biotechnology, Faculty of Advanced Medical Sciences, Stem Cell Research Center, Student Research Committee, and Drug Applied Research Center, Tabriz University of Medical Sciences, 5154853431 Tabriz, Iran. Department of Tissue Engineering, Faculty of Advanced Medical Sciences, Department of Nanotechnology, Faculty of Advanced Medical Sciences, Department of Biotechnology, Faculty of Advanced Medical Sciences, Stem Cell Research Center, Student Research Committee, and Drug Applied Research Center, Tabriz University of Medical Sciences, 5154853431 Tabriz, Iran.Department of Chemical Engineering, Northeastern University, Boston, Massachusetts 02115, United States.Department of Tissue Engineering, Faculty of Advanced Medical Sciences, Department of Nanotechnology, Faculty of Advanced Medical Sciences, Department of Biotechnology, Faculty of Advanced Medical Sciences, Stem Cell Research Center, Student Research Committee, and Drug Applied Research Center, Tabriz University of Medical Sciences, 5154853431 Tabriz, Iran. Department of Tissue Engineering, Faculty of Advanced Medical Sciences, Department of Nanotechnology, Faculty of Advanced Medical Sciences, Department of Biotechnology, Faculty of Advanced Medical Sciences, Stem Cell Research Center, Student Research Committee, and Drug Applied Research Center, Tabriz University of Medical Sciences, 5154853431 Tabriz, Iran. Department of Tissue Engineering, Faculty of Advanced Medical Sciences, Department of Nanotechnology, Faculty of Advanced Medical Sciences, Department of Biotechnology, Faculty of Advanced Medical Sciences, Stem Cell Research Center, Student Research Committee, and Drug Applied Research Center, Tabriz University of Medical Sciences, 5154853431 Tabriz, Iran.Department of Tissue Engineering, Faculty of Advanced Medical Sciences, Department of Nanotechnology, Faculty of Advanced Medical Sciences, Department of Biotechnology, Faculty of Advanced Medical Sciences, Stem Cell Research Center, Student Research Committee, and Drug Applied Research Center, Tabriz University of Medical Sciences, 5154853431 Tabriz, Iran.Department of Tissue Engineering, Faculty of Advanced Medical Sciences, Department of Nanotechnology, Faculty of Advanced Medical Sciences, Department of Biotechnology, Faculty of Advanced Medical Sciences, Stem Cell Research Center, Student Research Committee, and Drug Applied Research Center, Tabriz University of Medical Sciences, 5154853431 Tabriz, Iran. Department of Chemical Engineering, Northeastern University, Boston, Massachusetts 02115, United States. Department of Tissue Engineering, Faculty of Advanced Medical Sciences, Department of Nanotechnology, Faculty of Advanced Medical Sciences, Department of Biotechnology, Faculty of Advanced Medical Sciences, Stem Cell Research Center, Student Research Committee, and Drug Applied Research Center, Tabriz University of Medical Sciences, 5154853431 Tabriz, Iran.Department of Tissue Engineering, Faculty of Advanced Medical Sciences, Department of Nanotechnology, Faculty of Advanced Medical Sciences, Department of Biotechnology, Faculty of Advanced Medical Sciences, Stem Cell Research Center, Student Research Committee, and Drug Applied Research Center, Tabriz University of Medical Sciences, 5154853431 Tabriz, Iran.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31458990

Citation

Rahmani Del Bakhshayesh, Azizeh, et al. "Fabrication of Three-Dimensional Scaffolds Based On Nano-biomimetic Collagen Hybrid Constructs for Skin Tissue Engineering." ACS Omega, vol. 3, no. 8, 2018, pp. 8605-8611.
Rahmani Del Bakhshayesh A, Mostafavi E, Alizadeh E, et al. Fabrication of Three-Dimensional Scaffolds Based on Nano-biomimetic Collagen Hybrid Constructs for Skin Tissue Engineering. ACS Omega. 2018;3(8):8605-8611.
Rahmani Del Bakhshayesh, A., Mostafavi, E., Alizadeh, E., Asadi, N., Akbarzadeh, A., & Davaran, S. (2018). Fabrication of Three-Dimensional Scaffolds Based on Nano-biomimetic Collagen Hybrid Constructs for Skin Tissue Engineering. ACS Omega, 3(8), 8605-8611. https://doi.org/10.1021/acsomega.8b01219
Rahmani Del Bakhshayesh A, et al. Fabrication of Three-Dimensional Scaffolds Based On Nano-biomimetic Collagen Hybrid Constructs for Skin Tissue Engineering. ACS Omega. 2018 Aug 31;3(8):8605-8611. PubMed PMID: 31458990.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Fabrication of Three-Dimensional Scaffolds Based on Nano-biomimetic Collagen Hybrid Constructs for Skin Tissue Engineering. AU - Rahmani Del Bakhshayesh,Azizeh, AU - Mostafavi,Ebrahim, AU - Alizadeh,Effat, AU - Asadi,Nahideh, AU - Akbarzadeh,Abolfazl, AU - Davaran,Soodabeh, Y1 - 2018/08/02/ PY - 2018/06/03/received PY - 2018/07/05/accepted PY - 2019/8/29/entrez PY - 2019/8/29/pubmed PY - 2019/8/29/medline SP - 8605 EP - 8611 JF - ACS omega JO - ACS Omega VL - 3 IS - 8 N2 - Three-dimensional (3D) biodegradable and biomimetic porous scaffolds are ideal frameworks for skin tissue engineering. In this study, hybrid constructs of 3D scaffolds were successfully fabricated by the freeze-drying method from combinations of the type I collagen (Col) and synthetic poly(lactic acid) (PLLA) or polycaprolactone (PCL). Four different groups of 3D porous scaffolds including PCL, PCL-Col, PCL-PLLA, and PCL-PLLA-Col were fabricated and systematically characterized by hydrogen nuclear magnetic resonance, Fourier transform infrared spectroscopy, and scanning electron microscopy (SEM). Adipose tissue-derived mesenchymal stem cells (AT-MSCs) were seeded in all scaffolds, and the viability, proliferation, and adhesion of the cells were investigated using dimethylthiazol diphenyltetrazolium bromide assay and SEM. The results showed that scaffolds containing Col, particularly PCL-PLLA-Col scaffold, with pore sizes close to 400 nm and being sufficiently interconnected, have significantly greater potential (p < 0.01) for encouraging AT-MSCs adhesion and growth. The PCL-PLLA provided a mechanically stronger mesh support, and the type I Col microsponges encouraged excellent cell adhesion and tissue formation. The scaffold with the best properties could be an appropriate functional candidate for the preparation of artificial skin constructs. SN - 2470-1343 UR - https://www.unboundmedicine.com/medline/citation/31458990/Fabrication_of_Three_Dimensional_Scaffolds_Based_on_Nano_biomimetic_Collagen_Hybrid_Constructs_for_Skin_Tissue_Engineering_ DB - PRIME DP - Unbound Medicine ER -
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