Tags

Type your tag names separated by a space and hit enter

Sequence-based mapping identifies a candidate transcription repressor underlying awn suppression at the B1 locus in wheat.
New Phytol. 2020 01; 225(1):326-339.NP

Abstract

Awns are stiff, hair-like structures which grow from the lemmas of wheat (Triticum aestivum) and other grasses that contribute to photosynthesis and play a role in seed dispersal. Variation in awn length in domesticated wheat is controlled primarily by three major genes, most commonly the dominant awn suppressor Tipped1 (B1). This study identifies a transcription repressor responsible for awn inhibition at the B1 locus. Association mapping was combined with analysis in biparental populations to delimit B1 to a distal region of 5AL colocalized with QTL for number of spikelets per spike, kernel weight, kernel length, and test weight. Fine-mapping located B1 to a region containing only two predicted genes, including C2H2 zinc finger transcriptional repressor TraesCS5A02G542800 upregulated in developing spikes of awnless individuals. Deletions encompassing this candidate gene were present in awned mutants of an awnless wheat. Sequence polymorphisms in the B1 coding region were not observed in diverse wheat germplasm whereas a nearby polymorphism was highly predictive of awn suppression. Transcriptional repression by B1 is the major determinant of awn suppression in global wheat germplasm. It is associated with increased number of spikelets per spike and decreased kernel size.

Links

Authors+Show Affiliations

Department of Crop and Soil Sciences, North Carolina State University, Raleigh, NC, 27695, USA.

Department of Crop and Soil Sciences, North Carolina State University, Raleigh, NC, 27695, USA.

Department of Crop and Soil Sciences, North Carolina State University, Raleigh, NC, 27695, USA.

Department of Crop and Soil Sciences, North Carolina State University, Raleigh, NC, 27695, USA.

Department of Crop and Soil Sciences, North Carolina State University, Raleigh, NC, 27695, USA.

Department of Plant Sciences, University of Idaho, Moscow, ID, 83844, USA.

Department of Plant Sciences, University of Idaho, Moscow, ID, 83844, USA.

Department of Crop and Soil Sciences, North Carolina State University, Raleigh, NC, 27695, USA.

Department of Crop and Soil Sciences, North Carolina State University, Raleigh, NC, 27695, USA. USDA-ARS SAA, Plant Science Research, Raleigh, NC, 27695, USA.

Department of Plant Pathology, Kansas State University, Manhattan, KS, 66506, USA.

Department of Plant Pathology, Kansas State University, Manhattan, KS, 66506, USA.

Department of Plant Pathology, Kansas State University, Manhattan, KS, 66506, USA.

Brown-Guedira G

Department of Crop and Soil Sciences, North Carolina State University, Raleigh, NC, 27695, USA. USDA-ARS SAA, Plant Science Research, Raleigh, NC, 27695, USA.

Pub Type(s)

Journal Article

Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

31465541

Citation

DeWitt, Noah, et al. "Sequence-based Mapping Identifies a Candidate Transcription Repressor Underlying Awn Suppression at the B1 Locus in Wheat." The New Phytologist, vol. 225, no. 1, 2020, pp. 326-339.

DeWitt N, Guedira M, Lauer E, et al. Sequence-based mapping identifies a candidate transcription repressor underlying awn suppression at the B1 locus in wheat. New Phytol. 2020;225(1):326-339.

DeWitt, N., Guedira, M., Lauer, E., Sarinelli, M., Tyagi, P., Fu, D., Hao, Q., Murphy, J. P., Marshall, D., Akhunova, A., Jordan, K., Akhunov, E., & Brown-Guedira, G. (2020). Sequence-based mapping identifies a candidate transcription repressor underlying awn suppression at the B1 locus in wheat. The New Phytologist, 225(1), 326-339. https://doi.org/10.1111/nph.16152

DeWitt N, et al. Sequence-based Mapping Identifies a Candidate Transcription Repressor Underlying Awn Suppression at the B1 Locus in Wheat. New Phytol. 2020;225(1):326-339. PubMed PMID: 31465541.

* Article titles in AMA citation format should be in sentence-case

TY - JOUR T1 - Sequence-based mapping identifies a candidate transcription repressor underlying awn suppression at the B1 locus in wheat. AU - DeWitt,Noah, AU - Guedira,Mohammed, AU - Lauer,Edwin, AU - Sarinelli,Martin, AU - Tyagi,Priyanka, AU - Fu,Daolin, AU - Hao,QunQun, AU - Murphy,J Paul, AU - Marshall,David, AU - Akhunova,Alina, AU - Jordan,Katherine, AU - Akhunov,Eduard, AU - Brown-Guedira,Gina, Y1 - 2019/09/27/ PY - 2019/05/15/received PY - 2019/08/16/accepted PY - 2019/8/30/pubmed PY - 2020/11/12/medline PY - 2019/8/30/entrez KW - B1 locus KW - awns KW - fine mapping KW - positional cloning KW - wheat (Triticum aestivum) KW - zinc finger protein SP - 326 EP - 339 JF - The New phytologist JO - New Phytol VL - 225 IS - 1 N2 - Awns are stiff, hair-like structures which grow from the lemmas of wheat (Triticum aestivum) and other grasses that contribute to photosynthesis and play a role in seed dispersal. Variation in awn length in domesticated wheat is controlled primarily by three major genes, most commonly the dominant awn suppressor Tipped1 (B1). This study identifies a transcription repressor responsible for awn inhibition at the B1 locus. Association mapping was combined with analysis in biparental populations to delimit B1 to a distal region of 5AL colocalized with QTL for number of spikelets per spike, kernel weight, kernel length, and test weight. Fine-mapping located B1 to a region containing only two predicted genes, including C2H2 zinc finger transcriptional repressor TraesCS5A02G542800 upregulated in developing spikes of awnless individuals. Deletions encompassing this candidate gene were present in awned mutants of an awnless wheat. Sequence polymorphisms in the B1 coding region were not observed in diverse wheat germplasm whereas a nearby polymorphism was highly predictive of awn suppression. Transcriptional repression by B1 is the major determinant of awn suppression in global wheat germplasm. It is associated with increased number of spikelets per spike and decreased kernel size. SN - 1469-8137 UR - https://www.unboundmedicine.com/medline/citation/31465541/Sequence_based_mapping_identifies_a_candidate_transcription_repressor_underlying_awn_suppression_at_the_B1_locus_in_wheat_ DB - PRIME DP - Unbound Medicine ER -