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Diagnosing ovarian cancer by identifying SCC-antigen on a multiwalled carbon nanotube-modified dielectrode sensor.

Abstract

Ovarian cancer starts in the ovaries in its earlier stages and then spreads to the pelvis, uterus, and abdominal region. The success of an ovarian cancer treatment depends on the stage of the cancer and the diagnostic system. Squamous cell carcinoma antigen (SCC-Ag) is one of the most efficient cancer biomarkers, and elevated levels of SCC-Ag in ovarian cancer cells have been used to identify ovarian cancer. Carbon is a potential material for biosensing applications due to its thermal, electrical, and physical properties. Multiwalled carbon nanotubes (MWCNTs) are carbon-based materials that can be used here to detect SCC-Ag. Anti-SCC-Ag antibody was immobilized on the amine-modified MWCNT dielectric sensing surface to detect SCC-Ag. The uniformity of the surface structure was measured with a 3D nanoprofiler, and the results confirmed the detection of SCC-Ag at ∼80 pM. The specific detection of SCC-Ag was confirmed with two control proteins (factor IX and human serum albumin), and the system did not show biofouling. This experimental set-up with MWCNTs a dielectric sensing surface can lead to the detection of ovarian cancer in its initial stages.

Authors+Show Affiliations

Biomedical Isotope Research Center, School of Basic Medical Sciences, Shandong University, Jinan, Shandong Province, People's Republic of China.Department of Gynaecology and Obstetrics, Yuncheng County Traditional Chinese Medicine Hospital, Heze, Shandong Province, People's Republic of China.Maternal and Child Health Care of Shandong Province, Jinan, Shandong Province, People's Republic of China.School of Bioprocess Engineering, Universiti Malaysia Perlis, Arau, Perlis, Malaysia. Institute of Nano Electronic Engineering, Universiti Malaysia Perlis, Kangar, Perlis, Malaysia.Maternal and Child Health Care of Shandong Province, Jinan, Shandong Province, People's Republic of China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31468573

Citation

Liang, Ting, et al. "Diagnosing Ovarian Cancer By Identifying SCC-antigen On a Multiwalled Carbon Nanotube-modified Dielectrode Sensor." Biotechnology and Applied Biochemistry, 2019.
Liang T, Qu Q, Chang Y, et al. Diagnosing ovarian cancer by identifying SCC-antigen on a multiwalled carbon nanotube-modified dielectrode sensor. Biotechnol Appl Biochem. 2019.
Liang, T., Qu, Q., Chang, Y., Gopinath, S. C. B., & Liu, X. T. (2019). Diagnosing ovarian cancer by identifying SCC-antigen on a multiwalled carbon nanotube-modified dielectrode sensor. Biotechnology and Applied Biochemistry, doi:10.1002/bab.1808.
Liang T, et al. Diagnosing Ovarian Cancer By Identifying SCC-antigen On a Multiwalled Carbon Nanotube-modified Dielectrode Sensor. Biotechnol Appl Biochem. 2019 Aug 29; PubMed PMID: 31468573.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Diagnosing ovarian cancer by identifying SCC-antigen on a multiwalled carbon nanotube-modified dielectrode sensor. AU - Liang,Ting, AU - Qu,Qiuzhi, AU - Chang,Yuhua, AU - Gopinath,Subash Chandra Bose, AU - Liu,Xian Tong, Y1 - 2019/08/29/ PY - 2019/05/30/received PY - 2019/08/23/accepted PY - 2019/8/31/pubmed PY - 2019/8/31/medline PY - 2019/8/31/entrez KW - biosensor KW - cervical cancer KW - interdigitated electrode KW - multiwalled carbon nanotube surface KW - ovarian cancer biomarker JF - Biotechnology and applied biochemistry JO - Biotechnol. Appl. Biochem. N2 - Ovarian cancer starts in the ovaries in its earlier stages and then spreads to the pelvis, uterus, and abdominal region. The success of an ovarian cancer treatment depends on the stage of the cancer and the diagnostic system. Squamous cell carcinoma antigen (SCC-Ag) is one of the most efficient cancer biomarkers, and elevated levels of SCC-Ag in ovarian cancer cells have been used to identify ovarian cancer. Carbon is a potential material for biosensing applications due to its thermal, electrical, and physical properties. Multiwalled carbon nanotubes (MWCNTs) are carbon-based materials that can be used here to detect SCC-Ag. Anti-SCC-Ag antibody was immobilized on the amine-modified MWCNT dielectric sensing surface to detect SCC-Ag. The uniformity of the surface structure was measured with a 3D nanoprofiler, and the results confirmed the detection of SCC-Ag at ∼80 pM. The specific detection of SCC-Ag was confirmed with two control proteins (factor IX and human serum albumin), and the system did not show biofouling. This experimental set-up with MWCNTs a dielectric sensing surface can lead to the detection of ovarian cancer in its initial stages. SN - 1470-8744 UR - https://www.unboundmedicine.com/medline/citation/31468573/Diagnosing_ovarian_cancer_by_identifying_SCC-antigen_on_a_multiwalled_carbon_nanotube-modified_dielectrode_sensor L2 - https://doi.org/10.1002/bab.1808 DB - PRIME DP - Unbound Medicine ER -