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Modulatory effects of artichoke (Cynara scolymus L.) leaf extract against oxidative stress and hepatic TNF-α gene expression in acute diazinon-induced liver injury in rats.
J Basic Clin Physiol Pharmacol. 2019 Aug 30; 30(5)JB

Abstract

Background Diazinon (DZN) causes serious liver damage in both humans and animals. In the present study, the hepatoprotective effects of Cynara scolymus L. leaf extract against DZN-induced liver injury were examined. Methods Forty male rats were divided into five groups. The control group received a normal diet. The DZN group received DZN only (25 mg/kg, po). The DZN + Syl group received DZN (25 mg/kg, po) and silymarin (Syl) (50 mg/kg, po). The DZN + Art group received DZN (25 mg/kg, po) and artichoke (Art) leaf extract (1500 mg/kg, po). The Art group received Art leaf extract only (1500 mg/kg, po). After 15 days, serum tumor necrosis factor α (TNF-α), alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), lipid profile, protein carbonyl content, serum and hepatic malondialdehyde (MDA), hepatic TNF-α gene expression, hepatic catalase (CAT), superoxide dismutase (SOD), and vitamin C (Vit C) were measured and histopathological examination was performed. Results DZN caused a significant elevation in serum ALP, AST, ALT, MDA, TNF-α, protein carbonyl, hepatic MDA, and TNF-α gene expression in the DZN group as opposed to the control group. Also, DZN led to the reduction of hepatic CAT, SOD, and Vit C in the DZN group relative to the control group. The administration of Art extract resulted in not only a significant reduction in serum ALP, AST, ALT, MDA, TNF-α, and protein carbonyl but also an improvement of liver histopathological changes and hepatic CAT and SOD activities as opposed to the DZN group. Conclusions This study confirmed that Art leaf extract has liver protective effects and causes downregulation of oxidative stress in acute DZN-induced liver injury in rats.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Ahmadi A
Clinical Biochemistry Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran.
Heidarian E
Clinical Biochemistry Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran, Mobile: + 98 913 314 5229, Phone: + 98 383 3346720, Fax: + 98 383 3346721.
Ghatreh-Samani K
Cellular and Molecular Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran.

MeSH

AnimalsChemical and Drug Induced Liver InjuryCynara scolymusDiazinonEthanolGene ExpressionLiver Function TestsMaleOxidative StressPlant ExtractsPlant LeavesProtective AgentsRatsSilymarinTumor Necrosis Factor-alpha

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31469651

Citation

Ahmadi, Arezoo, et al. "Modulatory Effects of Artichoke (Cynara Scolymus L.) Leaf Extract Against Oxidative Stress and Hepatic TNF-α Gene Expression in Acute Diazinon-induced Liver Injury in Rats." Journal of Basic and Clinical Physiology and Pharmacology, vol. 30, no. 5, 2019.
Ahmadi A, Heidarian E, Ghatreh-Samani K. Modulatory effects of artichoke (Cynara scolymus L.) leaf extract against oxidative stress and hepatic TNF-α gene expression in acute diazinon-induced liver injury in rats. J Basic Clin Physiol Pharmacol. 2019;30(5).
Ahmadi, A., Heidarian, E., & Ghatreh-Samani, K. (2019). Modulatory effects of artichoke (Cynara scolymus L.) leaf extract against oxidative stress and hepatic TNF-α gene expression in acute diazinon-induced liver injury in rats. Journal of Basic and Clinical Physiology and Pharmacology, 30(5). https://doi.org/10.1515/jbcpp-2018-0180
Ahmadi A, Heidarian E, Ghatreh-Samani K. Modulatory Effects of Artichoke (Cynara Scolymus L.) Leaf Extract Against Oxidative Stress and Hepatic TNF-α Gene Expression in Acute Diazinon-induced Liver Injury in Rats. J Basic Clin Physiol Pharmacol. 2019 Aug 30;30(5) PubMed PMID: 31469651.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Modulatory effects of artichoke (Cynara scolymus L.) leaf extract against oxidative stress and hepatic TNF-α gene expression in acute diazinon-induced liver injury in rats. AU - Ahmadi,Arezoo, AU - Heidarian,Esfandiar, AU - Ghatreh-Samani,Keihan, Y1 - 2019/08/30/ PY - 2018/10/11/received PY - 2019/06/18/accepted PY - 2019/8/31/pubmed PY - 2020/2/23/medline PY - 2019/8/31/entrez KW - Cynara scolymus L KW - TNF-α KW - artichoke KW - diazinon KW - liver damage KW - oxidative stress JF - Journal of basic and clinical physiology and pharmacology JO - J Basic Clin Physiol Pharmacol VL - 30 IS - 5 N2 - Background Diazinon (DZN) causes serious liver damage in both humans and animals. In the present study, the hepatoprotective effects of Cynara scolymus L. leaf extract against DZN-induced liver injury were examined. Methods Forty male rats were divided into five groups. The control group received a normal diet. The DZN group received DZN only (25 mg/kg, po). The DZN + Syl group received DZN (25 mg/kg, po) and silymarin (Syl) (50 mg/kg, po). The DZN + Art group received DZN (25 mg/kg, po) and artichoke (Art) leaf extract (1500 mg/kg, po). The Art group received Art leaf extract only (1500 mg/kg, po). After 15 days, serum tumor necrosis factor α (TNF-α), alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), lipid profile, protein carbonyl content, serum and hepatic malondialdehyde (MDA), hepatic TNF-α gene expression, hepatic catalase (CAT), superoxide dismutase (SOD), and vitamin C (Vit C) were measured and histopathological examination was performed. Results DZN caused a significant elevation in serum ALP, AST, ALT, MDA, TNF-α, protein carbonyl, hepatic MDA, and TNF-α gene expression in the DZN group as opposed to the control group. Also, DZN led to the reduction of hepatic CAT, SOD, and Vit C in the DZN group relative to the control group. The administration of Art extract resulted in not only a significant reduction in serum ALP, AST, ALT, MDA, TNF-α, and protein carbonyl but also an improvement of liver histopathological changes and hepatic CAT and SOD activities as opposed to the DZN group. Conclusions This study confirmed that Art leaf extract has liver protective effects and causes downregulation of oxidative stress in acute DZN-induced liver injury in rats. SN - 2191-0286 UR - https://www.unboundmedicine.com/medline/citation/31469651/Modulatory_effects_of_artichoke__Cynara_scolymus_L___leaf_extract_against_oxidative_stress_and_hepatic_TNF_α_gene_expression_in_acute_diazinon_induced_liver_injury_in_rats_ DB - PRIME DP - Unbound Medicine ER -