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Analysis of the 56-kDa type specific antigen gene of Orientia tsutsugamushi from northern Vietnam.
PLoS One. 2019; 14(8):e0221588.Plos

Abstract

Scrub typhus has been documented since 1932 in Vietnam, however, the disease burden of scrub typhus remains poorly understood in the country. We conducted this study to describe the phylogenetic analysis of the 56-kDa type-specific antigen (TSA) gene of Orientia tsutsugamushi associated with PCR positive cases of scrub typhus. Of 116 positive samples, 65 type-specific antigen gene sequences were obtained and classified into 3 genogroups: Karp, Kato and Gilliam. The Karp genogroup was the most frequently detected phylogenetic cluster in the study with 30 samples (46%), followed by Kato and Gilliam with 20 (31%) and 15 (23%), respectively. All sequences showed 94-100% nucleotide similarity to reference sequences collected in the central part of Vietnam in 2017. Patients infected with Karp genogroup were more likely to have significant thrombocytopenia than the other genogroups. These results suggest that any scrub typhus vaccine considered for use in Vietnam should provide protection against each of these 3 genogroups.

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Authors+Show Affiliations

Trung NV
National Hospital for Tropical Diseases, Hanoi, Vietnam. Hanoi Medical University, Hanoi, Vietnam.
Hoi LT
National Hospital for Tropical Diseases, Hanoi, Vietnam.
Cuong DD
Bach Mai Hospital, Hanoi, Vietnam.
Ha DT
Hanoi Medical University, Hanoi, Vietnam.
Hoa TM
National Hospital for Tropical Diseases, Hanoi, Vietnam.
Lien VN
National Hospital for Tropical Diseases, Hanoi, Vietnam.
Hoa NT
National Hospital for Tropical Diseases, Hanoi, Vietnam.
Hoa LNM
National Hospital for Tropical Diseases, Hanoi, Vietnam.
Huong DT
National Hospital for Tropical Diseases, Hanoi, Vietnam.
Bich VTN
Oxford University Clinical Research Unit, Wellcome Trust Asia Program, Hanoi, Vietnam.
van Doorn HR
Oxford University Clinical Research Unit, Wellcome Trust Asia Program, Hanoi, Vietnam.
Nadjm B
Oxford University Clinical Research Unit, Wellcome Trust Asia Program, Hanoi, Vietnam.
Richards AL
Naval Medical Research Center, Silver Spring, Maryland, United States of America. Uniformed Services University of the Health Sciences, Bethesda, Maryland, United States of America.

MeSH

Antigens, BacterialBacterial ProteinsGenotypeHigh-Throughput Nucleotide SequencingHumansMolecular TypingMolecular WeightOrientia tsutsugamushiPhylogenyPhylogeographyPrevalenceScrub TyphusSequence Analysis, DNASeverity of Illness IndexVietnam

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

31469878

Citation

Trung, Nguyen Vu, et al. "Analysis of the 56-kDa Type Specific Antigen Gene of Orientia Tsutsugamushi From Northern Vietnam." PloS One, vol. 14, no. 8, 2019, pp. e0221588.
Trung NV, Hoi LT, Cuong DD, et al. Analysis of the 56-kDa type specific antigen gene of Orientia tsutsugamushi from northern Vietnam. PLoS One. 2019;14(8):e0221588.
Trung, N. V., Hoi, L. T., Cuong, D. D., Ha, D. T., Hoa, T. M., Lien, V. N., Hoa, N. T., Hoa, L. N. M., Huong, D. T., Bich, V. T. N., van Doorn, H. R., Nadjm, B., & Richards, A. L. (2019). Analysis of the 56-kDa type specific antigen gene of Orientia tsutsugamushi from northern Vietnam. PloS One, 14(8), e0221588. https://doi.org/10.1371/journal.pone.0221588
Trung NV, et al. Analysis of the 56-kDa Type Specific Antigen Gene of Orientia Tsutsugamushi From Northern Vietnam. PLoS One. 2019;14(8):e0221588. PubMed PMID: 31469878.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Analysis of the 56-kDa type specific antigen gene of Orientia tsutsugamushi from northern Vietnam. AU - Trung,Nguyen Vu, AU - Hoi,Le Thi, AU - Cuong,Do Duy, AU - Ha,Doan The, AU - Hoa,Tran Mai, AU - Lien,Vu Ngoc, AU - Hoa,Nguyen Thi, AU - Hoa,Le Nguyen Minh, AU - Huong,Dang Thi, AU - Bich,Vu Thi Ngoc, AU - van Doorn,H Rogier, AU - Nadjm,Behzad, AU - Richards,Allen L, Y1 - 2019/08/30/ PY - 2019/04/03/received PY - 2019/08/09/accepted PY - 2019/8/31/entrez PY - 2019/8/31/pubmed PY - 2020/3/12/medline SP - e0221588 EP - e0221588 JF - PloS one JO - PLoS One VL - 14 IS - 8 N2 - Scrub typhus has been documented since 1932 in Vietnam, however, the disease burden of scrub typhus remains poorly understood in the country. We conducted this study to describe the phylogenetic analysis of the 56-kDa type-specific antigen (TSA) gene of Orientia tsutsugamushi associated with PCR positive cases of scrub typhus. Of 116 positive samples, 65 type-specific antigen gene sequences were obtained and classified into 3 genogroups: Karp, Kato and Gilliam. The Karp genogroup was the most frequently detected phylogenetic cluster in the study with 30 samples (46%), followed by Kato and Gilliam with 20 (31%) and 15 (23%), respectively. All sequences showed 94-100% nucleotide similarity to reference sequences collected in the central part of Vietnam in 2017. Patients infected with Karp genogroup were more likely to have significant thrombocytopenia than the other genogroups. These results suggest that any scrub typhus vaccine considered for use in Vietnam should provide protection against each of these 3 genogroups. SN - 1932-6203 UR - https://www.unboundmedicine.com/medline/citation/31469878/Analysis_of_the_56_kDa_type_specific_antigen_gene_of_Orientia_tsutsugamushi_from_northern_Vietnam_ DB - PRIME DP - Unbound Medicine ER -