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Evaluation of the roflumilast effect supplemented with linezolid in pleural empyema in rats caused by intrapleural S.aureus inoculation.

Abstract

In addition to tube drains, pleural empyema is treated with antibiotics and anti-inflammatory drugs. We aimed to evaluate the anti-inflammatory activity of roflumilast combined with linezolid in a rat model of pleural empyema induced by Staphylococcus aureus.A total of 40 rats were separated into 7 groups: Sham (n=4), S. aureus inoculation (n=6), S. aureus + 10 mg/kg linezolid (n=6), S. aureus + 5 mg/kg roflumilast (n=6), S. aureus + 10 mg/kg linezolid+5 mg/kg roflumilast (n=6), S. aureus + 10 mg/kg roflumilast (n=6), and S. aureus + 10 mg/kg linezolid + 10 mg/kg roflumilast (n=6). Linezolid was administered 1 hour before and 12 hours after inoculation with S. aureus. Roflumilast was administered orally as a single dose 30 minutes before inoculation with S. aureus. Compared to linezolid alone, linezolid combined with 5 mg/kg roflumilast provided statistically significant improvement in TNF-alpha, IL-1 beta, vasodilation/congestion, and tissue/pleural PNM infiltration (p<0.05).Linezolid combined with 10 mg/kg roflumilast also provided statistically significant improvement TNF-alpha, IL-1 beta, IL-6, endothelin-1, vasodilation/congestion, mesothelial cell damage, lung tissue PNL, and pleural PNL compared to linezolid alone (p<0.05).Due to its anti-inflammatory effect and significant impact on recovery, roflumilast can be used in conjunction with antibiotherapy for the treatment of pleural empyema.

Authors+Show Affiliations

Health Sciences University Erzurum Regional Education and Research Hospital, Depertmant of Pulmonary Diseases.Ataturk University School of Medicine, Department of Pulmonary Diseases.Health Sciences University Erzurum Regional Education and Research Hospital, Depertmant of Infection Diseases and Clinical Microbiology.Kastamonu University Faculty of Veterinary, Department of Biochemistry.Ataturk University School of Medicine, Department of Pathology.Ataturk University School of Medicine, Department of Pharmacology.Ataturk University School of Medicine, Department of Pulmonary Diseases.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31474702

Citation

Kerget, Buğra, et al. "Evaluation of the Roflumilast Effect Supplemented With Linezolid in Pleural Empyema in Rats Caused By Intrapleural S.aureus Inoculation." Japanese Journal of Infectious Diseases, 2019.
Kerget B, Araz Ö, Kerget F, et al. Evaluation of the roflumilast effect supplemented with linezolid in pleural empyema in rats caused by intrapleural S.aureus inoculation. Jpn J Infect Dis. 2019.
Kerget, B., Araz, Ö., Kerget, F., Erol, H. S., Özmen, S., Halıcı, Z., & Akgün, M. (2019). Evaluation of the roflumilast effect supplemented with linezolid in pleural empyema in rats caused by intrapleural S.aureus inoculation. Japanese Journal of Infectious Diseases, doi:10.7883/yoken.JJID.2019.164.
Kerget B, et al. Evaluation of the Roflumilast Effect Supplemented With Linezolid in Pleural Empyema in Rats Caused By Intrapleural S.aureus Inoculation. Jpn J Infect Dis. 2019 Aug 30; PubMed PMID: 31474702.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Evaluation of the roflumilast effect supplemented with linezolid in pleural empyema in rats caused by intrapleural S.aureus inoculation. AU - Kerget,Buğra, AU - Araz,Ömer, AU - Kerget,Ferhan, AU - Erol,Hüseyin Serkan, AU - Özmen,Sevilay, AU - Halıcı,Zekai, AU - Akgün,Metin, Y1 - 2019/08/30/ PY - 2019/9/3/entrez KW - Linezolid KW - lung tissue KW - pleural empyema KW - treatment JF - Japanese journal of infectious diseases JO - Jpn. J. Infect. Dis. N2 - In addition to tube drains, pleural empyema is treated with antibiotics and anti-inflammatory drugs. We aimed to evaluate the anti-inflammatory activity of roflumilast combined with linezolid in a rat model of pleural empyema induced by Staphylococcus aureus.A total of 40 rats were separated into 7 groups: Sham (n=4), S. aureus inoculation (n=6), S. aureus + 10 mg/kg linezolid (n=6), S. aureus + 5 mg/kg roflumilast (n=6), S. aureus + 10 mg/kg linezolid+5 mg/kg roflumilast (n=6), S. aureus + 10 mg/kg roflumilast (n=6), and S. aureus + 10 mg/kg linezolid + 10 mg/kg roflumilast (n=6). Linezolid was administered 1 hour before and 12 hours after inoculation with S. aureus. Roflumilast was administered orally as a single dose 30 minutes before inoculation with S. aureus. Compared to linezolid alone, linezolid combined with 5 mg/kg roflumilast provided statistically significant improvement in TNF-alpha, IL-1 beta, vasodilation/congestion, and tissue/pleural PNM infiltration (p<0.05).Linezolid combined with 10 mg/kg roflumilast also provided statistically significant improvement TNF-alpha, IL-1 beta, IL-6, endothelin-1, vasodilation/congestion, mesothelial cell damage, lung tissue PNL, and pleural PNL compared to linezolid alone (p<0.05).Due to its anti-inflammatory effect and significant impact on recovery, roflumilast can be used in conjunction with antibiotherapy for the treatment of pleural empyema. SN - 1884-2836 UR - https://www.unboundmedicine.com/medline/citation/31474702/Evaluation_of_the_roflumilast_effect_supplemented_with_linezolid_in_pleural_empyema_in_rats_caused_by_intrapleural_S.aureus_inoculation L2 - https://dx.doi.org/10.7883/yoken.JJID.2019.164 DB - PRIME DP - Unbound Medicine ER -