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Microfluidic capillary zone electrophoresis mass spectrometry analysis of alkaloids in Lobelia cardinalis transgenic and mutant plant cell cultures.

Abstract

Application of a microfluidic CE* device for CZE-MS allows for fast, rapid, and in-depth analysis of large sample sets. This microfluidic CZE-MS device, the 908 Devices ZipChip, involves minimal sample preparation and is ideal for small cation analytes, such as alkaloids. Here, we evaluated the microfluidic device for the analysis of alkaloids from Lobelia cardinalis hairy root cultures. Extracts from wild-type, transgenic, and selected mutant plant cultures were analyzed and data batch processed using the mass spectral processing software MZmine2 and the statistical software Prism 8. In total 139 features were detected as baseline resolved peaks via the MZmine2 software optimized for the electrophoretic separations. Statistically significant differences in the relative abundance of the primary alkaloid lobinaline (C27 H34 N2), along with several putative "lobinaline-like" molecules were observed utilizing this approach. Additionally, a method for performing both targeted and untargeted MS/MS experiments using the microfluidic device was developed and evaluated. Coupling data-processing software with CZE-MS data acquisition has enabled comprehensive metabolomic profiles from plant cell cultures to be constructed within a single working day.

Authors+Show Affiliations

Department of Chemistry, University of Kentucky, Lexington, KY, USA.Naprogenix Inc., Lexington, KY, USA.Department of Psychology, University of Kentucky, Lexington, KY, USA. Naprogenix Inc., Lexington, KY, USA.Department of Chemistry, University of Kentucky, Lexington, KY, USA.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31475363

Citation

Kelley, Zachary D., et al. "Microfluidic Capillary Zone Electrophoresis Mass Spectrometry Analysis of Alkaloids in Lobelia Cardinalis Transgenic and Mutant Plant Cell Cultures." Electrophoresis, 2019.
Kelley ZD, Rogers DT, Littleton JM, et al. Microfluidic capillary zone electrophoresis mass spectrometry analysis of alkaloids in Lobelia cardinalis transgenic and mutant plant cell cultures. Electrophoresis. 2019.
Kelley, Z. D., Rogers, D. T., Littleton, J. M., & Lynn, B. C. (2019). Microfluidic capillary zone electrophoresis mass spectrometry analysis of alkaloids in Lobelia cardinalis transgenic and mutant plant cell cultures. Electrophoresis, doi:10.1002/elps.201900220.
Kelley ZD, et al. Microfluidic Capillary Zone Electrophoresis Mass Spectrometry Analysis of Alkaloids in Lobelia Cardinalis Transgenic and Mutant Plant Cell Cultures. Electrophoresis. 2019 Sep 1; PubMed PMID: 31475363.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Microfluidic capillary zone electrophoresis mass spectrometry analysis of alkaloids in Lobelia cardinalis transgenic and mutant plant cell cultures. AU - Kelley,Zachary D, AU - Rogers,D Trent, AU - Littleton,John M, AU - Lynn,Bert C, Y1 - 2019/09/01/ PY - 2019/06/19/received PY - 2019/08/09/revised PY - 2019/08/19/accepted PY - 2019/9/3/pubmed PY - 2019/9/3/medline PY - 2019/9/3/entrez KW - Alkaloid KW - Bioinformatics KW - Microfluidic KW - Tandem MS JF - Electrophoresis JO - Electrophoresis N2 - Application of a microfluidic CE* device for CZE-MS allows for fast, rapid, and in-depth analysis of large sample sets. This microfluidic CZE-MS device, the 908 Devices ZipChip, involves minimal sample preparation and is ideal for small cation analytes, such as alkaloids. Here, we evaluated the microfluidic device for the analysis of alkaloids from Lobelia cardinalis hairy root cultures. Extracts from wild-type, transgenic, and selected mutant plant cultures were analyzed and data batch processed using the mass spectral processing software MZmine2 and the statistical software Prism 8. In total 139 features were detected as baseline resolved peaks via the MZmine2 software optimized for the electrophoretic separations. Statistically significant differences in the relative abundance of the primary alkaloid lobinaline (C27 H34 N2), along with several putative "lobinaline-like" molecules were observed utilizing this approach. Additionally, a method for performing both targeted and untargeted MS/MS experiments using the microfluidic device was developed and evaluated. Coupling data-processing software with CZE-MS data acquisition has enabled comprehensive metabolomic profiles from plant cell cultures to be constructed within a single working day. SN - 1522-2683 UR - https://www.unboundmedicine.com/medline/citation/31475363/Microfluidic_capillary_zone_electrophoresis_mass_spectrometry_analysis_of_alkaloids_in_Lobelia_cardinalis_transgenic_and_mutant_plant_cell_cultures L2 - https://doi.org/10.1002/elps.201900220 DB - PRIME DP - Unbound Medicine ER -