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[Electroacupuncture improves insomnia by down-regulating peripheral benzodiazepine receptor expression in hippocampus, and up-regulating 5-HT, 5-HIAA, TNF-α and IL-1β contents in hypothalamus in insomnia rats].
Zhen Ci Yan Jiu 2019; 44(8):560-5ZC

Abstract

OBJECTIVE

To observe the therapeutic effect of electroacupuncture (EA) at five Back-Shu points on sleep, hippocampal peripheral benzodiazepine receptor (PBR) expression and hypothalamic 5-hydroxytryptamine (5-HT), 5-hydroxyindoleacetic acid (5-HIAA), tumor necrosis factor alpha (TNF-α) and interleukin (IL)-1β contents in insomnia rats, so as to explore its mechanisms underlying improvement of insomnia.

METHODS

Forty male SD rats were randomly divided into control, model, EA and medication (Diazepam) groups (n=10 rats in each group). The insomnia model was established by intraperitoneal injection (i.p.) of para-Chlorophenylalanine (PCPA, 300 mg/kg) once daily for 2 days. EA (60 Hz, 1 mA) was applied to bilateral five Back-Shu points, i.e., Feishu (BL13), Xinshu (BL15), Ganshu (BL18), Pishu (BL20) and Shenshu (BL23) for 10 min, once daily for 6 days. Rats of the medication group were treated by gavage of Diazepam (0.92 mg/kg) once daily for 6 days. The sleep duration was recorded after i.p. of Pentobarbital Sodium (45 mg/kg). Histopathological changes of the hippocampus were displayed by H.E. staining. The contents of 5-HT, 5-HIAA, TNF-α and IL-1β in the hypothalamus were assessed by using ELISA. The expression levels of PBR mRNA and protein in the hippocampus were detected by quantitative real-time PCR, immunohistochemistry and Western blot, separately.

RESULTS

Following modeling, the sleep duration was considerably shortened in rats of the model group relevant to the control group (P<0.05). After the treatment, the sleep duration was significantly increased in both EA and medication groups relevant to the model group (P<0.05). After modeling, the hippocampal neurons were obviously decreased in number, and disordered in the arrangement, the levels of hypothalamic 5-HT, 5-HIAA, TNF-α, and IL-1β were significantly down-regulated (P<0.05), and the expression of PBR mRNA and protein in hippocampus was obviously increased relevant to the control group (P<0.05). Following the treatment, the hippocampal neurons were increased in number and arranged regularly, and the contents of hypothalamic 5-HT, 5-HIAA, TNF-α, and IL-1β were considerably increased (P<0.05), and the expression of PBR mRNA and protein in hippocampus was significantly decreased in both EA and medication groups relevant to the model group (P<0.05). The therapeutic effect of EA was comparable to that of medication in increasing sleep duration, body weight, 5-HT and 5-HIAA contents (P>0.05), and significantly superior to that of the medication in increasing TNF-α and IL-1β levels (P<0.05), and considerably superior to that of medication in down-regulating PBR mRNA and protein expression (P<0.05).

CONCLUSION

EA at five Back-Shu-points of the five Zang-organs can significantly improve the sleep in insomnia rats, which is closely associated with its effects in reducing the expression of PBR in hippocampus and up-regulating the levels of 5-HT, 5-HIAA, TNF-α and IL-1β in hypothalamus.

Authors+Show Affiliations

Department of Rehabilitation, The Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430014, China.Department of Rehabilitation, The Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430014, China.Department of Rehabilitation, The Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430014, China.Department of Rehabilitation, The Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430014, China.

Pub Type(s)

Journal Article

Language

chi

PubMed ID

31475488

Citation

Tang, Lei, et al. "[Electroacupuncture Improves Insomnia By Down-regulating Peripheral Benzodiazepine Receptor Expression in Hippocampus, and Up-regulating 5-HT, 5-HIAA, TNF-α and IL-1β Contents in Hypothalamus in Insomnia Rats]." Zhen Ci Yan Jiu = Acupuncture Research, vol. 44, no. 8, 2019, pp. 560-5.
Tang L, You F, Hu X, et al. [Electroacupuncture improves insomnia by down-regulating peripheral benzodiazepine receptor expression in hippocampus, and up-regulating 5-HT, 5-HIAA, TNF-α and IL-1β contents in hypothalamus in insomnia rats]. Zhen Ci Yan Jiu. 2019;44(8):560-5.
Tang, L., You, F., Hu, X., & Li, Y. F. (2019). [Electroacupuncture improves insomnia by down-regulating peripheral benzodiazepine receptor expression in hippocampus, and up-regulating 5-HT, 5-HIAA, TNF-α and IL-1β contents in hypothalamus in insomnia rats]. Zhen Ci Yan Jiu = Acupuncture Research, 44(8), pp. 560-5. doi:10.13702/j.1000-0607.180610.
Tang L, et al. [Electroacupuncture Improves Insomnia By Down-regulating Peripheral Benzodiazepine Receptor Expression in Hippocampus, and Up-regulating 5-HT, 5-HIAA, TNF-α and IL-1β Contents in Hypothalamus in Insomnia Rats]. Zhen Ci Yan Jiu. 2019 Aug 25;44(8):560-5. PubMed PMID: 31475488.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Electroacupuncture improves insomnia by down-regulating peripheral benzodiazepine receptor expression in hippocampus, and up-regulating 5-HT, 5-HIAA, TNF-α and IL-1β contents in hypothalamus in insomnia rats]. AU - Tang,Lei, AU - You,Fei, AU - Hu,Xing, AU - Li,Yun-Fei, PY - 2019/9/3/entrez PY - 2019/9/3/pubmed PY - 2019/9/11/medline KW - Electroacupuncture KW - Five Back-Shu points KW - Insomnia KW - Peripheral benzodiazepine receptor SP - 560 EP - 5 JF - Zhen ci yan jiu = Acupuncture research JO - Zhen Ci Yan Jiu VL - 44 IS - 8 N2 - OBJECTIVE: To observe the therapeutic effect of electroacupuncture (EA) at five Back-Shu points on sleep, hippocampal peripheral benzodiazepine receptor (PBR) expression and hypothalamic 5-hydroxytryptamine (5-HT), 5-hydroxyindoleacetic acid (5-HIAA), tumor necrosis factor alpha (TNF-α) and interleukin (IL)-1β contents in insomnia rats, so as to explore its mechanisms underlying improvement of insomnia. METHODS: Forty male SD rats were randomly divided into control, model, EA and medication (Diazepam) groups (n=10 rats in each group). The insomnia model was established by intraperitoneal injection (i.p.) of para-Chlorophenylalanine (PCPA, 300 mg/kg) once daily for 2 days. EA (60 Hz, 1 mA) was applied to bilateral five Back-Shu points, i.e., Feishu (BL13), Xinshu (BL15), Ganshu (BL18), Pishu (BL20) and Shenshu (BL23) for 10 min, once daily for 6 days. Rats of the medication group were treated by gavage of Diazepam (0.92 mg/kg) once daily for 6 days. The sleep duration was recorded after i.p. of Pentobarbital Sodium (45 mg/kg). Histopathological changes of the hippocampus were displayed by H.E. staining. The contents of 5-HT, 5-HIAA, TNF-α and IL-1β in the hypothalamus were assessed by using ELISA. The expression levels of PBR mRNA and protein in the hippocampus were detected by quantitative real-time PCR, immunohistochemistry and Western blot, separately. RESULTS: Following modeling, the sleep duration was considerably shortened in rats of the model group relevant to the control group (P<0.05). After the treatment, the sleep duration was significantly increased in both EA and medication groups relevant to the model group (P<0.05). After modeling, the hippocampal neurons were obviously decreased in number, and disordered in the arrangement, the levels of hypothalamic 5-HT, 5-HIAA, TNF-α, and IL-1β were significantly down-regulated (P<0.05), and the expression of PBR mRNA and protein in hippocampus was obviously increased relevant to the control group (P<0.05). Following the treatment, the hippocampal neurons were increased in number and arranged regularly, and the contents of hypothalamic 5-HT, 5-HIAA, TNF-α, and IL-1β were considerably increased (P<0.05), and the expression of PBR mRNA and protein in hippocampus was significantly decreased in both EA and medication groups relevant to the model group (P<0.05). The therapeutic effect of EA was comparable to that of medication in increasing sleep duration, body weight, 5-HT and 5-HIAA contents (P>0.05), and significantly superior to that of the medication in increasing TNF-α and IL-1β levels (P<0.05), and considerably superior to that of medication in down-regulating PBR mRNA and protein expression (P<0.05). CONCLUSION: EA at five Back-Shu-points of the five Zang-organs can significantly improve the sleep in insomnia rats, which is closely associated with its effects in reducing the expression of PBR in hippocampus and up-regulating the levels of 5-HT, 5-HIAA, TNF-α and IL-1β in hypothalamus. SN - 1000-0607 UR - https://www.unboundmedicine.com/medline/citation/31475488/[Electroacupuncture_improves_insomnia_by_down_regulating_peripheral_benzodiazepine_receptor_expression_in_hippocampus_and_up_regulating_5_HT_5_HIAA_TNF_α_and_IL_1β_contents_in_hypothalamus_in_insomnia_rats]_ DB - PRIME DP - Unbound Medicine ER -