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[Effect of herbal cake-partitioned moxibustion on renal function and expression of renal connective tissue growth factor, integrin-linked kinase and bone morphogenetic protein-7 in chronic renal failure rabbits].
Zhen Ci Yan Jiu. 2019 Aug 25; 44(8):577-82.ZC

Abstract

OBJECTIVE

To observe the effect of herbal cake-partitioned moxibustion on renal function and expression of connective tissue growth factor (CTGF), integrin-linked kinase (ILK) and bone morphogenetic protein-7 (BMP-7) in chronic renal failure (CRF) rabbits, so as to reveal its mechanisms underlying improvement of CRF.

METHODS

Twenty-four male New Zealand rabbits were randomly divided into control, model, medication and herbal cake-partitioned moxibustion (moxibustion) groups (n=6 rabbits in each group). The CRF model was established by gavage of suspension of Adenine (150 mg·kg-1·d-1) for 21 days. Herbal cake-partitioned moxibustion was applied to "Mingmen"(GV4) and bilateral "Shenshu"(BL23), "Pishu"(BL20) and for 5 moxa-cones every time. Rabbits of the medication group was treated by gavage of Losartan Potassium (2.33 mg·kg-1·d-1). All the treatments were conducted once daily,12 times a course for consecutive 3 courses with a two-day rest after each course of treatment. Serum creatinine (Scr), blood urea nitrogen (BUN) and 24 h urine protein contents were detected by using an automatic biochemical analyzer. The expression levels of CTGF, ILK and BMP-7 proteins and mRNA in the kidney tissue were determined by Western blot and quantitative real time-PCR, separately.

RESULTS

Following modeling, Scr and BUN and 24 h urine protein contents were significantly increased in the model group in comparison with the control group (P<0.01). After the intervention, Scr and BUN contents were all significantly decreased in both the moxibustion and medication groups relevant to the model group (P<0.01), suggesting an improvement of the renal function. Compared with the control group, the expression levels of ILK and CTGF mRNAs and proteins were obviously up-regulated (P<0.01), and those of BMP-7 mRNA and protein significantly decreased in the model group (P<0.01). In comparison with the model group, the expression levels of ILK and CTGF mRNAs and proteins were significantly down-regulated in the two treatment groups (P<0.01, P<0.05), and those of BMP-7 mRNA and protein markedly increased in the two treatment groups (P<0.01). In comparison with the medication group, the expression level of ILK protein was significantly up-regulated (P<0.01) and BMP-7 protein was significantly down-regulated (P<0.01) in the moxibustion group. No significant differences were found between the medication and moxibustion groups in down-regulating the levels of Scr, BUN and 24 h urine protein and expression of ILK mRNA, CTGF mRNA and protein and BMP-7 mRNA(P>0.05).

CONCLUSION

Herbal cake-partitioned moxibustion can improve renal function in CRF rabbits, which may be related to its effects in suppressing the expression of ILK and CTGF, and in up-regulating the expression of BMP-7 in the kidney tissue.

Authors+Show Affiliations

School of Acupuncture-moxibustion, Tuina and Rehabilitation, Yunnan University of Traditional Chinese Medicine, Kunming 650500, China.School of Acupuncture-moxibustion, Tuina and Rehabilitation, Yunnan University of Traditional Chinese Medicine, Kunming 650500, China.Clinical Medical College of Acupuncture-moxibustion and Rehabilitation, Guangzhou University of Chinese Medicine, Guangzhou 510405.School of Acupuncture-moxibustion, Tuina and Rehabilitation, Yunnan University of Traditional Chinese Medicine, Kunming 650500, China.School of Acupuncture-moxibustion, Tuina and Rehabilitation, Yunnan University of Traditional Chinese Medicine, Kunming 650500, China.Infirmary of Yunnan University, Kunming 650091.School of Acupuncture-moxibustion, Tuina and Rehabilitation, Yunnan University of Traditional Chinese Medicine, Kunming 650500, China; School of Acupuncture-moxibustion and Tuina, Beijing University of Chinese Medicine, Beijing 100029.

Pub Type(s)

Journal Article

Language

chi

PubMed ID

31475491

Citation

Huang, Pei-Dong, et al. "[Effect of Herbal Cake-partitioned Moxibustion On Renal Function and Expression of Renal Connective Tissue Growth Factor, Integrin-linked Kinase and Bone Morphogenetic Protein-7 in Chronic Renal Failure Rabbits]." Zhen Ci Yan Jiu = Acupuncture Research, vol. 44, no. 8, 2019, pp. 577-82.
Huang PD, Wang XY, Yi W, et al. [Effect of herbal cake-partitioned moxibustion on renal function and expression of renal connective tissue growth factor, integrin-linked kinase and bone morphogenetic protein-7 in chronic renal failure rabbits]. Zhen Ci Yan Jiu. 2019;44(8):577-82.
Huang, P. D., Wang, X. Y., Yi, W., Yuan, K., Ying, L., Dai, S., & Zuo, Z. (2019). [Effect of herbal cake-partitioned moxibustion on renal function and expression of renal connective tissue growth factor, integrin-linked kinase and bone morphogenetic protein-7 in chronic renal failure rabbits]. Zhen Ci Yan Jiu = Acupuncture Research, 44(8), 577-82. https://doi.org/10.13702/j.1000-0607.180719
Huang PD, et al. [Effect of Herbal Cake-partitioned Moxibustion On Renal Function and Expression of Renal Connective Tissue Growth Factor, Integrin-linked Kinase and Bone Morphogenetic Protein-7 in Chronic Renal Failure Rabbits]. Zhen Ci Yan Jiu. 2019 Aug 25;44(8):577-82. PubMed PMID: 31475491.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Effect of herbal cake-partitioned moxibustion on renal function and expression of renal connective tissue growth factor, integrin-linked kinase and bone morphogenetic protein-7 in chronic renal failure rabbits]. AU - Huang,Pei-Dong, AU - Wang,Xin-Yue, AU - Yi,Wei, AU - Yuan,Kai, AU - Ying,Li, AU - Dai,Shu, AU - Zuo,Zheng, PY - 2019/9/3/entrez PY - 2019/9/3/pubmed PY - 2019/9/11/medline KW - Blood urea nitrogen KW - Bone morphogenetic protein-7 KW - Chronic renal failure KW - Connective tissue growth factor KW - Herbal cake-partitioned moxibustion KW - Integrin-linked kinase KW - Serum creatinine SP - 577 EP - 82 JF - Zhen ci yan jiu = Acupuncture research JO - Zhen Ci Yan Jiu VL - 44 IS - 8 N2 - OBJECTIVE: To observe the effect of herbal cake-partitioned moxibustion on renal function and expression of connective tissue growth factor (CTGF), integrin-linked kinase (ILK) and bone morphogenetic protein-7 (BMP-7) in chronic renal failure (CRF) rabbits, so as to reveal its mechanisms underlying improvement of CRF. METHODS: Twenty-four male New Zealand rabbits were randomly divided into control, model, medication and herbal cake-partitioned moxibustion (moxibustion) groups (n=6 rabbits in each group). The CRF model was established by gavage of suspension of Adenine (150 mg·kg-1·d-1) for 21 days. Herbal cake-partitioned moxibustion was applied to "Mingmen"(GV4) and bilateral "Shenshu"(BL23), "Pishu"(BL20) and for 5 moxa-cones every time. Rabbits of the medication group was treated by gavage of Losartan Potassium (2.33 mg·kg-1·d-1). All the treatments were conducted once daily,12 times a course for consecutive 3 courses with a two-day rest after each course of treatment. Serum creatinine (Scr), blood urea nitrogen (BUN) and 24 h urine protein contents were detected by using an automatic biochemical analyzer. The expression levels of CTGF, ILK and BMP-7 proteins and mRNA in the kidney tissue were determined by Western blot and quantitative real time-PCR, separately. RESULTS: Following modeling, Scr and BUN and 24 h urine protein contents were significantly increased in the model group in comparison with the control group (P<0.01). After the intervention, Scr and BUN contents were all significantly decreased in both the moxibustion and medication groups relevant to the model group (P<0.01), suggesting an improvement of the renal function. Compared with the control group, the expression levels of ILK and CTGF mRNAs and proteins were obviously up-regulated (P<0.01), and those of BMP-7 mRNA and protein significantly decreased in the model group (P<0.01). In comparison with the model group, the expression levels of ILK and CTGF mRNAs and proteins were significantly down-regulated in the two treatment groups (P<0.01, P<0.05), and those of BMP-7 mRNA and protein markedly increased in the two treatment groups (P<0.01). In comparison with the medication group, the expression level of ILK protein was significantly up-regulated (P<0.01) and BMP-7 protein was significantly down-regulated (P<0.01) in the moxibustion group. No significant differences were found between the medication and moxibustion groups in down-regulating the levels of Scr, BUN and 24 h urine protein and expression of ILK mRNA, CTGF mRNA and protein and BMP-7 mRNA(P>0.05). CONCLUSION: Herbal cake-partitioned moxibustion can improve renal function in CRF rabbits, which may be related to its effects in suppressing the expression of ILK and CTGF, and in up-regulating the expression of BMP-7 in the kidney tissue. SN - 1000-0607 UR - https://www.unboundmedicine.com/medline/citation/31475491/[Effect_of_herbal_cake_partitioned_moxibustion_on_renal_function_and_expression_of_renal_connective_tissue_growth_factor_integrin_linked_kinase_and_bone_morphogenetic_protein_7_in_chronic_renal_failure_rabbits]_ DB - PRIME DP - Unbound Medicine ER -