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Expression and localization of the small proteoglycans decorin and biglycan in articular cartilage of Kashin-Beck disease and rats induced by T-2 toxin and selenium deficiency.
Glycoconj J. 2019 12; 36(6):451-459.GJ

Abstract

Kashin-Beck disease (KBD) is an endemic degenerative osteoarthropathy of uncertain etiology. Our study sought to identify a correlation between small proteoglycans decorin and biglycan expression and Kashin-Beck Disease. Immunohistochemistry was used to assess the decorin and biglycan levels in cartilage specimens from both child KBD patients, and rats fed with T-2 toxin under a selenium-deficient condition. Real-time PCR and Western blot were used to assess mRNA and protein levels of decorin and biglycan in rat cartilages, as well as in C28/I2 chondrocytes stimulated by T-2 toxin and selenium in vitro. The result showed that decorin was reduced in all zones of KBD articular cartilage, while the expression of biglycan was prominently increased in KBD cartilage samples. Increased expression of biglycan and reduced expression of decorin were observed at mRNA and protein levels in the cartilage of rats fed with T-2 toxin and selenium- deficiency plus T-2 toxin diet, when compared with the normal diet group. Moreover, In vitro stimulation of C28/I2 cells with T-2 toxin resulted in an upregulation of biglycan and downregulation of decorin, T-2 toxin induction of biglycan and decorin levels were partly rescued by selenium supplement. This study highlights the focal nature of the degenerative changes that occur in KBD cartilage and may suggest that the altered expression pattern of decorin and biglycan have an important role in the onset and pathogenesis of KBD.

Authors+Show Affiliations

School of Public Health, Xi'an Jiaotong University Health Science Center, Key Laboratory of Trace Elements and Endemic Diseases, National Health and Family Planning Commission, No. 76 Yanta West Road, Xi'an, 710061, Shaanxi, People's Republic of China.Xijing Hospital, Medical University of the Air Force, Xi'an, 710061, Shaanxi, People's Republic of China.School of Public Health, Xi'an Jiaotong University Health Science Center, Key Laboratory of Trace Elements and Endemic Diseases, National Health and Family Planning Commission, No. 76 Yanta West Road, Xi'an, 710061, Shaanxi, People's Republic of China.School of Public Health, Xi'an Jiaotong University Health Science Center, Key Laboratory of Trace Elements and Endemic Diseases, National Health and Family Planning Commission, No. 76 Yanta West Road, Xi'an, 710061, Shaanxi, People's Republic of China.School of Public Health, Xi'an Jiaotong University Health Science Center, Key Laboratory of Trace Elements and Endemic Diseases, National Health and Family Planning Commission, No. 76 Yanta West Road, Xi'an, 710061, Shaanxi, People's Republic of China.School of Public Health, Xi'an Jiaotong University Health Science Center, Key Laboratory of Trace Elements and Endemic Diseases, National Health and Family Planning Commission, No. 76 Yanta West Road, Xi'an, 710061, Shaanxi, People's Republic of China.School of Public Health, Xi'an Jiaotong University Health Science Center, Key Laboratory of Trace Elements and Endemic Diseases, National Health and Family Planning Commission, No. 76 Yanta West Road, Xi'an, 710061, Shaanxi, People's Republic of China. Department of Integrative Medical Biology, University of Umeå, Umeå, Sweden.School of Public Health, Xi'an Jiaotong University Health Science Center, Key Laboratory of Trace Elements and Endemic Diseases, National Health and Family Planning Commission, No. 76 Yanta West Road, Xi'an, 710061, Shaanxi, People's Republic of China.School of Public Health, Xi'an Jiaotong University Health Science Center, Key Laboratory of Trace Elements and Endemic Diseases, National Health and Family Planning Commission, No. 76 Yanta West Road, Xi'an, 710061, Shaanxi, People's Republic of China. chenjh.123@mail.xjtu.edu.cn.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

31478096

Citation

Wang, Mengying, et al. "Expression and Localization of the Small Proteoglycans Decorin and Biglycan in Articular Cartilage of Kashin-Beck Disease and Rats Induced By T-2 Toxin and Selenium Deficiency." Glycoconjugate Journal, vol. 36, no. 6, 2019, pp. 451-459.
Wang M, Xue S, Fang Q, et al. Expression and localization of the small proteoglycans decorin and biglycan in articular cartilage of Kashin-Beck disease and rats induced by T-2 toxin and selenium deficiency. Glycoconj J. 2019;36(6):451-459.
Wang, M., Xue, S., Fang, Q., Zhang, M., He, Y., Zhang, Y., Lammi, M. J., Cao, J., & Chen, J. (2019). Expression and localization of the small proteoglycans decorin and biglycan in articular cartilage of Kashin-Beck disease and rats induced by T-2 toxin and selenium deficiency. Glycoconjugate Journal, 36(6), 451-459. https://doi.org/10.1007/s10719-019-09889-9
Wang M, et al. Expression and Localization of the Small Proteoglycans Decorin and Biglycan in Articular Cartilage of Kashin-Beck Disease and Rats Induced By T-2 Toxin and Selenium Deficiency. Glycoconj J. 2019;36(6):451-459. PubMed PMID: 31478096.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Expression and localization of the small proteoglycans decorin and biglycan in articular cartilage of Kashin-Beck disease and rats induced by T-2 toxin and selenium deficiency. AU - Wang,Mengying, AU - Xue,Senhai, AU - Fang,Qian, AU - Zhang,Meng, AU - He,Ying, AU - Zhang,Ying, AU - Lammi,Mikko J, AU - Cao,Junling, AU - Chen,Jinghong, Y1 - 2019/09/02/ PY - 2019/06/01/received PY - 2019/08/13/accepted PY - 2019/07/31/revised PY - 2019/9/4/pubmed PY - 2020/5/16/medline PY - 2019/9/4/entrez KW - Biglycan KW - Decorin KW - Extracellular matrix KW - Kashin-Beck disease KW - Selenium KW - T-2 toxin SP - 451 EP - 459 JF - Glycoconjugate journal JO - Glycoconj. J. VL - 36 IS - 6 N2 - Kashin-Beck disease (KBD) is an endemic degenerative osteoarthropathy of uncertain etiology. Our study sought to identify a correlation between small proteoglycans decorin and biglycan expression and Kashin-Beck Disease. Immunohistochemistry was used to assess the decorin and biglycan levels in cartilage specimens from both child KBD patients, and rats fed with T-2 toxin under a selenium-deficient condition. Real-time PCR and Western blot were used to assess mRNA and protein levels of decorin and biglycan in rat cartilages, as well as in C28/I2 chondrocytes stimulated by T-2 toxin and selenium in vitro. The result showed that decorin was reduced in all zones of KBD articular cartilage, while the expression of biglycan was prominently increased in KBD cartilage samples. Increased expression of biglycan and reduced expression of decorin were observed at mRNA and protein levels in the cartilage of rats fed with T-2 toxin and selenium- deficiency plus T-2 toxin diet, when compared with the normal diet group. Moreover, In vitro stimulation of C28/I2 cells with T-2 toxin resulted in an upregulation of biglycan and downregulation of decorin, T-2 toxin induction of biglycan and decorin levels were partly rescued by selenium supplement. This study highlights the focal nature of the degenerative changes that occur in KBD cartilage and may suggest that the altered expression pattern of decorin and biglycan have an important role in the onset and pathogenesis of KBD. SN - 1573-4986 UR - https://www.unboundmedicine.com/medline/citation/31478096/Expression_and_localization_of_the_small_proteoglycans_decorin_and_biglycan_in_articular_cartilage_of_Kashin-Beck_disease_and_rats_induced_by_T-2_toxin_and_selenium_deficiency L2 - https://doi.org/10.1007/s10719-019-09889-9 DB - PRIME DP - Unbound Medicine ER -