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Skin-on-a-Chip Device for Ex Vivo Monitoring of Transdermal Delivery of Drugs-Design, Fabrication, and Testing.
Pharmaceutics 2019; 11(9)P

Abstract

To develop proper drug formulations and to optimize the delivery of their active ingredients through the dermal barrier, the Franz diffusion cell system is the most widely used in vitro/ex vivo technique. However, different providers and manufacturers make various types of this equipment (horizontal, vertical, static, flow-through, smaller and larger chambers, etc.) with high variability and not fully comparable and consistent data. Furthermore, a high amount of test drug formulations and large size of diffusion skin surface and membranes are important requirements for the application of these methods. The aim of our study was to develop a novel Microfluidic Diffusion Chamber device and compare it with the traditional techniques. Here the design, fabrication, and a pilot testing of a microfluidic skin-on-a chip device are described. Based on this chip, further developments can also be implemented for industrial purposes to assist the characterization and optimization of drug formulations, dermal pharmacokinetics, and pharmacodynamic studies. The advantages of our device, beside the low costs, are the small drug and skin consumption, low sample volumes, dynamic arrangement with continuous flow mimicking the dermal circulation, as well as rapid and reproducible results.

Authors+Show Affiliations

Faculty of Information Technology and Bionics, Pázmány Péter Catholic University, Práter u. 50a, H-1083 Budapest, Hungary.Faculty of Information Technology and Bionics, Pázmány Péter Catholic University, Práter u. 50a, H-1083 Budapest, Hungary.Faculty of Information Technology and Bionics, Pázmány Péter Catholic University, Práter u. 50a, H-1083 Budapest, Hungary.Biological Research Center, Hungarian Academy of Sciences, Temesvári krt. 62, H-6726 Szeged, Hungary.Department of Pharmaceutics, Semmelweis University, Hőgyes Endre u. 7, H-1092 Budapest, Hungary.Faculty of Information Technology and Bionics, Pázmány Péter Catholic University, Práter u. 50a, H-1083 Budapest, Hungary.Faculty of Information Technology and Bionics, Pázmány Péter Catholic University, Práter u. 50a, H-1083 Budapest, Hungary. Department of Biophysics and Radiation Biology, Semmelweis University, Tűzoltó u. 37-47. H-1094 Budapest, Hungary.Faculty of Information Technology and Bionics, Pázmány Péter Catholic University, Práter u. 50a, H-1083 Budapest, Hungary. erdo.franciska@itk.ppke.hu.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31480652

Citation

Lukács, Bence, et al. "Skin-on-a-Chip Device for Ex Vivo Monitoring of Transdermal Delivery of Drugs-Design, Fabrication, and Testing." Pharmaceutics, vol. 11, no. 9, 2019.
Lukács B, Bajza Á, Kocsis D, et al. Skin-on-a-Chip Device for Ex Vivo Monitoring of Transdermal Delivery of Drugs-Design, Fabrication, and Testing. Pharmaceutics. 2019;11(9).
Lukács, B., Bajza, Á., Kocsis, D., Csorba, A., Antal, I., Iván, K., ... Erdő, F. (2019). Skin-on-a-Chip Device for Ex Vivo Monitoring of Transdermal Delivery of Drugs-Design, Fabrication, and Testing. Pharmaceutics, 11(9), doi:10.3390/pharmaceutics11090445.
Lukács B, et al. Skin-on-a-Chip Device for Ex Vivo Monitoring of Transdermal Delivery of Drugs-Design, Fabrication, and Testing. Pharmaceutics. 2019 Sep 2;11(9) PubMed PMID: 31480652.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Skin-on-a-Chip Device for Ex Vivo Monitoring of Transdermal Delivery of Drugs-Design, Fabrication, and Testing. AU - Lukács,Bence, AU - Bajza,Ágnes, AU - Kocsis,Dorottya, AU - Csorba,Attila, AU - Antal,István, AU - Iván,Kristóf, AU - Laki,András József, AU - Erdő,Franciska, Y1 - 2019/09/02/ PY - 2019/08/09/received PY - 2019/08/26/revised PY - 2019/08/27/accepted PY - 2019/9/5/entrez PY - 2019/9/5/pubmed PY - 2019/9/5/medline KW - Franz diffusion cell KW - drug delivery KW - microfluidics KW - skin-on-a-chip KW - transdermal microdialysis JF - Pharmaceutics JO - Pharmaceutics VL - 11 IS - 9 N2 - To develop proper drug formulations and to optimize the delivery of their active ingredients through the dermal barrier, the Franz diffusion cell system is the most widely used in vitro/ex vivo technique. However, different providers and manufacturers make various types of this equipment (horizontal, vertical, static, flow-through, smaller and larger chambers, etc.) with high variability and not fully comparable and consistent data. Furthermore, a high amount of test drug formulations and large size of diffusion skin surface and membranes are important requirements for the application of these methods. The aim of our study was to develop a novel Microfluidic Diffusion Chamber device and compare it with the traditional techniques. Here the design, fabrication, and a pilot testing of a microfluidic skin-on-a chip device are described. Based on this chip, further developments can also be implemented for industrial purposes to assist the characterization and optimization of drug formulations, dermal pharmacokinetics, and pharmacodynamic studies. The advantages of our device, beside the low costs, are the small drug and skin consumption, low sample volumes, dynamic arrangement with continuous flow mimicking the dermal circulation, as well as rapid and reproducible results. SN - 1999-4923 UR - https://www.unboundmedicine.com/medline/citation/31480652/Skin_on_a_Chip_Device_for_Ex_Vivo_Monitoring_of_Transdermal_Delivery_of_Drugs_Design_Fabrication_and_Testing_ L2 - http://www.mdpi.com/resolver?pii=pharmaceutics11090445 DB - PRIME DP - Unbound Medicine ER -