Tags

Type your tag names separated by a space and hit enter

Dissection of loci conferring resistance to stripe rust in Chinese wheat landraces from the middle and lower reaches of the Yangtze River via genome-wide association study.
Plant Sci. 2019 Oct; 287:110204.PS

Abstract

Stripe rust (Yr), caused by the fungal pathogen Puccinia striiformis f. sp. tritici, is a devastating foliar disease of wheat in China. Chinese wheat landraces originating from the middle and lower reaches of the Yangtze River are potential stripe-rust resistance resources. To identify APR genes for stripe-rust resistance, a panel of 188 accessions derived from the middle and lower reaches of the Yangtze River were inoculated with a mixture of Chinese P. striiformis f. sp. tritici races and resistance evaluated under field conditions in five environments at adult-plant stages. Seventy-three accessions showed degrees of stable resistance. Combining phenotypic datasets from multiple field experiments with high-quality Diversity Arrays Technology and simple sequence repeat markers, we detected 21 marker-trait associations spanning 18 quantitative trait loci (QTLs) on chromosomes 1B, 2A, 2B, 3B, 4A, 5A, 5B, 6B, and 6D, respectively. Single QTLs explained 9.67% to 16.14% of the observed phenotypic variation. Nine QTLs co-localized with previously reported Yr genes or genomic regions. The remaining QTLs were potential novel loci associated with adult-stage stripe-rust resistance. Two novel QTLs, QYr.sicau-3B.2 and QYr.sicau-5B.3, located on chromosomes 3B and 5B significantly explained 16.14% and 11.16% of the phenotypic variation, respectively. Haplotype analysis revealed that accessions carrying APR variants or their combinations showed enhanced degrees of resistance. The potentially novel loci or genomic regions associated with adult-stage resistance may be useful to improve stripe-rust resistance in current wheat cultivars and for future isolation of stripe-rust resistance genes.

Authors+Show Affiliations

Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, 611130, PR China.Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, 611130, PR China.Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, 611130, PR China.Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, 611130, PR China.Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, 611130, PR China.Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, 611130, PR China.Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, 611130, PR China.Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, 611130, PR China.Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, 611130, PR China.Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, 611130, PR China.Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, 611130, PR China.College of Agronomy, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, 611130, PR China.Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, 611130, PR China.Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, 611130, PR China.Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, 611130, PR China.Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, 611130, PR China.Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, 611130, PR China.US Department of Agriculture, Agricultural Research Service, Wheat Health, Genetics and Quality Research Unit, USA; Department of Plant Pathology, Washington State University, Pullman, WA 99164-6430, USA.Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, 611130, PR China.Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, 611130, PR China. Electronic address: ymwei@sicau.edu.cn.Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, 611130, PR China. Electronic address: gychen@sicau.edu.cn.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31481207

Citation

Cheng, Yukun, et al. "Dissection of Loci Conferring Resistance to Stripe Rust in Chinese Wheat Landraces From the Middle and Lower Reaches of the Yangtze River Via Genome-wide Association Study." Plant Science : an International Journal of Experimental Plant Biology, vol. 287, 2019, p. 110204.
Cheng Y, Li J, Yao F, et al. Dissection of loci conferring resistance to stripe rust in Chinese wheat landraces from the middle and lower reaches of the Yangtze River via genome-wide association study. Plant Sci. 2019;287:110204.
Cheng, Y., Li, J., Yao, F., Long, L., Wang, Y., Wu, Y., Li, J., Ye, X., Wang, J., Jiang, Q., Kang, H., Li, W., Qi, P., Liu, Y., Deng, M., Ma, J., Jiang, Y., Chen, X., Zheng, Y., ... Chen, G. (2019). Dissection of loci conferring resistance to stripe rust in Chinese wheat landraces from the middle and lower reaches of the Yangtze River via genome-wide association study. Plant Science : an International Journal of Experimental Plant Biology, 287, 110204. https://doi.org/10.1016/j.plantsci.2019.110204
Cheng Y, et al. Dissection of Loci Conferring Resistance to Stripe Rust in Chinese Wheat Landraces From the Middle and Lower Reaches of the Yangtze River Via Genome-wide Association Study. Plant Sci. 2019;287:110204. PubMed PMID: 31481207.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Dissection of loci conferring resistance to stripe rust in Chinese wheat landraces from the middle and lower reaches of the Yangtze River via genome-wide association study. AU - Cheng,Yukun, AU - Li,Jian, AU - Yao,Fangjie, AU - Long,Li, AU - Wang,Yuqi, AU - Wu,Yu, AU - Li,Jing, AU - Ye,Xueling, AU - Wang,Jirui, AU - Jiang,Qiantao, AU - Kang,Houyang, AU - Li,Wei, AU - Qi,Pengfei, AU - Liu,Yaxi, AU - Deng,Mei, AU - Ma,Jian, AU - Jiang,Yunfeng, AU - Chen,Xianming, AU - Zheng,Youliang, AU - Wei,Yuming, AU - Chen,Guoyue, Y1 - 2019/07/27/ PY - 2019/05/31/received PY - 2019/07/24/revised PY - 2019/07/25/accepted PY - 2019/9/5/entrez PY - 2019/9/5/pubmed PY - 2019/12/24/medline KW - Adult-plant resistance (APR) KW - Diversity Arrays Technology (DArT) KW - Genome-wide association study (GWAS) KW - Simple sequence repeat (SSR) KW - Stripe rust KW - Wheat landrace SP - 110204 EP - 110204 JF - Plant science : an international journal of experimental plant biology JO - Plant Sci VL - 287 N2 - Stripe rust (Yr), caused by the fungal pathogen Puccinia striiformis f. sp. tritici, is a devastating foliar disease of wheat in China. Chinese wheat landraces originating from the middle and lower reaches of the Yangtze River are potential stripe-rust resistance resources. To identify APR genes for stripe-rust resistance, a panel of 188 accessions derived from the middle and lower reaches of the Yangtze River were inoculated with a mixture of Chinese P. striiformis f. sp. tritici races and resistance evaluated under field conditions in five environments at adult-plant stages. Seventy-three accessions showed degrees of stable resistance. Combining phenotypic datasets from multiple field experiments with high-quality Diversity Arrays Technology and simple sequence repeat markers, we detected 21 marker-trait associations spanning 18 quantitative trait loci (QTLs) on chromosomes 1B, 2A, 2B, 3B, 4A, 5A, 5B, 6B, and 6D, respectively. Single QTLs explained 9.67% to 16.14% of the observed phenotypic variation. Nine QTLs co-localized with previously reported Yr genes or genomic regions. The remaining QTLs were potential novel loci associated with adult-stage stripe-rust resistance. Two novel QTLs, QYr.sicau-3B.2 and QYr.sicau-5B.3, located on chromosomes 3B and 5B significantly explained 16.14% and 11.16% of the phenotypic variation, respectively. Haplotype analysis revealed that accessions carrying APR variants or their combinations showed enhanced degrees of resistance. The potentially novel loci or genomic regions associated with adult-stage resistance may be useful to improve stripe-rust resistance in current wheat cultivars and for future isolation of stripe-rust resistance genes. SN - 1873-2259 UR - https://www.unboundmedicine.com/medline/citation/31481207/Dissection_of_loci_conferring_resistance_to_stripe_rust_in_Chinese_wheat_landraces_from_the_middle_and_lower_reaches_of_the_Yangtze_River_via_genome_wide_association_study_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0168-9452(19)30719-8 DB - PRIME DP - Unbound Medicine ER -