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Analysis of Phenolic Composition of Byproducts (Seeds and Peels) of Avocado (Persea americana Mill.) Cultivated in Colombia.
Molecules 2019; 24(17)M

Abstract

The byproducts (seeds and peels) of an avocado cultivated in the south of Colombia were extracted with aqueous acetone and their antioxidant properties were measured with ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) and DPPH (2,2-diphenyl-1-picrylhydrazyl) assays, and total polyphenol content was determined by Folin-Ciocalteu method. A bioguided fractionation was performed, first by SPE (solid phase extraction) on Amberlite XAD-7, and then by size exclusion chromatography on Sephadex LH-20. The polyphenolic-rich extracts and their fractions were analyzed by ultra-performance liquid chromatography-electrospray ionization-mass spectrometry (UPLC-ESI-MS/MS), finding the presence of organic acids, hydroxycinnamic acids, catechins, free and glycosylated flavonoids, and dimeric and trimeric procyanidins. Catechin, epicatechin, six quercetin derivatives, four dimeric procyanidins (three type B and one type A), and three trimeric procyanidins (two type B and one type A) were detected in the most active fractions of avocado peel and seeds. The most antioxidant fractions contain the higher molecular weight phenolic compounds (condensed tannins).

Authors+Show Affiliations

Departamento de Química, Universidad de Nariño, Pasto AA 1175, Nariño, Colombia.Departamento de Química, Universidad de Nariño, Pasto AA 1175, Nariño, Colombia.Departamento de Química, Universidad Nacional de Colombia, Bogotá DC AA 14490, Colombia.Departamento de Química, Universidad de Nariño, Pasto AA 1175, Nariño, Colombia. nhurtado@udenar.edu.co.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31484471

Citation

Rosero, Johanna C., et al. "Analysis of Phenolic Composition of Byproducts (Seeds and Peels) of Avocado (Persea Americana Mill.) Cultivated in Colombia." Molecules (Basel, Switzerland), vol. 24, no. 17, 2019.
Rosero JC, Cruz S, Osorio C, et al. Analysis of Phenolic Composition of Byproducts (Seeds and Peels) of Avocado (Persea americana Mill.) Cultivated in Colombia. Molecules. 2019;24(17).
Rosero, J. C., Cruz, S., Osorio, C., & Hurtado, N. (2019). Analysis of Phenolic Composition of Byproducts (Seeds and Peels) of Avocado (Persea americana Mill.) Cultivated in Colombia. Molecules (Basel, Switzerland), 24(17), doi:10.3390/molecules24173209.
Rosero JC, et al. Analysis of Phenolic Composition of Byproducts (Seeds and Peels) of Avocado (Persea Americana Mill.) Cultivated in Colombia. Molecules. 2019 Sep 3;24(17) PubMed PMID: 31484471.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Analysis of Phenolic Composition of Byproducts (Seeds and Peels) of Avocado (Persea americana Mill.) Cultivated in Colombia. AU - Rosero,Johanna C, AU - Cruz,Silvia, AU - Osorio,Coralia, AU - Hurtado,Nelson, Y1 - 2019/09/03/ PY - 2019/07/03/received PY - 2019/07/26/revised PY - 2019/07/28/accepted PY - 2019/9/6/entrez PY - 2019/9/6/pubmed PY - 2020/1/28/medline KW - DPPH KW - Trolox equivalent antioxidant capacity (TEAC) KW - UPLC-ESI-MS/MS KW - bio-guided fractionation KW - polyphenols JF - Molecules (Basel, Switzerland) JO - Molecules VL - 24 IS - 17 N2 - The byproducts (seeds and peels) of an avocado cultivated in the south of Colombia were extracted with aqueous acetone and their antioxidant properties were measured with ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) and DPPH (2,2-diphenyl-1-picrylhydrazyl) assays, and total polyphenol content was determined by Folin-Ciocalteu method. A bioguided fractionation was performed, first by SPE (solid phase extraction) on Amberlite XAD-7, and then by size exclusion chromatography on Sephadex LH-20. The polyphenolic-rich extracts and their fractions were analyzed by ultra-performance liquid chromatography-electrospray ionization-mass spectrometry (UPLC-ESI-MS/MS), finding the presence of organic acids, hydroxycinnamic acids, catechins, free and glycosylated flavonoids, and dimeric and trimeric procyanidins. Catechin, epicatechin, six quercetin derivatives, four dimeric procyanidins (three type B and one type A), and three trimeric procyanidins (two type B and one type A) were detected in the most active fractions of avocado peel and seeds. The most antioxidant fractions contain the higher molecular weight phenolic compounds (condensed tannins). SN - 1420-3049 UR - https://www.unboundmedicine.com/medline/citation/31484471/Analysis_of_Phenolic_Composition_of_Byproducts__Seeds_and_Peels__of_Avocado__Persea_americana_Mill___Cultivated_in_Colombia_ L2 - http://www.mdpi.com/resolver?pii=molecules24173209 DB - PRIME DP - Unbound Medicine ER -