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HilD induces expression of a novel Salmonella Typhimurium invasion factor, YobH, through a regulatory cascade involving SprB.
Sci Rep. 2019 09 04; 9(1):12725.SR

Abstract

HilD is an AraC-like transcriptional regulator encoded in the Salmonella pathogenicity island 1 (SPI-1), which actives transcription of many genes within and outside SPI-1 that are mainly required for invasion of Salmonella into host cells. HilD controls expression of target genes directly or by acting through distinct regulators; three different regulatory cascades headed by HilD have been described to date. Here, by analyzing the effect of HilD on the yobH gene in Salmonella enterica serovar Typhimurium (S. Typhimurium), we further define an additional regulatory cascade mediated by HilD, which was revealed by previous genome-wide analyses. In this regulatory cascade, HilD acts through SprB, a LuxR-like regulator encoded in SPI-1, to induce expression of virulence genes. Our data show that HilD induces expression of sprB by directly counteracting H-NS-mediated repression on the promoter region upstream of this gene. Then, SprB directly activates expression of several genes including yobH, slrP and ugtL. Interestingly, we found that YobH, a protein of only 79 amino acids, is required for invasion of S. Typhimurium into HeLa cells and mouse macrophages. Thus, our results reveal a novel S. Typhimurium invasion factor and provide more evidence supporting the HilD-SprB regulatory cascade.

Authors+Show Affiliations

Departamento de Microbiología Molecular, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Morelos, 62210, Mexico.Departamento de Microbiología Molecular, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Morelos, 62210, Mexico.Departamento de Microbiología Molecular, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Morelos, 62210, Mexico. victor@ibt.unam.mx.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

31484980

Citation

Banda, María M., et al. "HilD Induces Expression of a Novel Salmonella Typhimurium Invasion Factor, YobH, Through a Regulatory Cascade Involving SprB." Scientific Reports, vol. 9, no. 1, 2019, p. 12725.
Banda MM, Manzo R, Bustamante VH. HilD induces expression of a novel Salmonella Typhimurium invasion factor, YobH, through a regulatory cascade involving SprB. Sci Rep. 2019;9(1):12725.
Banda, M. M., Manzo, R., & Bustamante, V. H. (2019). HilD induces expression of a novel Salmonella Typhimurium invasion factor, YobH, through a regulatory cascade involving SprB. Scientific Reports, 9(1), 12725. https://doi.org/10.1038/s41598-019-49192-z
Banda MM, Manzo R, Bustamante VH. HilD Induces Expression of a Novel Salmonella Typhimurium Invasion Factor, YobH, Through a Regulatory Cascade Involving SprB. Sci Rep. 2019 09 4;9(1):12725. PubMed PMID: 31484980.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - HilD induces expression of a novel Salmonella Typhimurium invasion factor, YobH, through a regulatory cascade involving SprB. AU - Banda,María M, AU - Manzo,Rubiceli, AU - Bustamante,Víctor H, Y1 - 2019/09/04/ PY - 2019/05/07/received PY - 2019/08/20/accepted PY - 2019/9/6/entrez PY - 2019/9/6/pubmed PY - 2020/11/5/medline SP - 12725 EP - 12725 JF - Scientific reports JO - Sci Rep VL - 9 IS - 1 N2 - HilD is an AraC-like transcriptional regulator encoded in the Salmonella pathogenicity island 1 (SPI-1), which actives transcription of many genes within and outside SPI-1 that are mainly required for invasion of Salmonella into host cells. HilD controls expression of target genes directly or by acting through distinct regulators; three different regulatory cascades headed by HilD have been described to date. Here, by analyzing the effect of HilD on the yobH gene in Salmonella enterica serovar Typhimurium (S. Typhimurium), we further define an additional regulatory cascade mediated by HilD, which was revealed by previous genome-wide analyses. In this regulatory cascade, HilD acts through SprB, a LuxR-like regulator encoded in SPI-1, to induce expression of virulence genes. Our data show that HilD induces expression of sprB by directly counteracting H-NS-mediated repression on the promoter region upstream of this gene. Then, SprB directly activates expression of several genes including yobH, slrP and ugtL. Interestingly, we found that YobH, a protein of only 79 amino acids, is required for invasion of S. Typhimurium into HeLa cells and mouse macrophages. Thus, our results reveal a novel S. Typhimurium invasion factor and provide more evidence supporting the HilD-SprB regulatory cascade. SN - 2045-2322 UR - https://www.unboundmedicine.com/medline/citation/31484980/HilD_induces_expression_of_a_novel_Salmonella_Typhimurium_invasion_factor_YobH_through_a_regulatory_cascade_involving_SprB_ L2 - https://doi.org/10.1038/s41598-019-49192-z DB - PRIME DP - Unbound Medicine ER -