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Standardized Emblica officinalis fruit extract inhibited the activities of α-amylase, α-glucosidase, and dipeptidyl peptidase-4 and displayed antioxidant potential.
J Sci Food Agric. 2020 Jan 30; 100(2):509-516.JS

Abstract

BACKGROUND

Emblica officinalis, known as amla in Ayurveda, has been used as a folk medicine to treat numerous pathological conditions, including diabetes. However, the novel extract of E. officinalis fruit extract (amla fruit extract, AFE, Saberry®) containing 100 g kg-1 β-glucogallin along with hydrolyzable tannins has not yet been extensively studied for its antidiabetic potential.

OBJECTIVE

The aim of this study was to investigate the antidiabetic and antioxidant activities of AFE and its stability during gastric stress as well as its thermostability.

METHODS

The effect of AFE on the inhibition of pancreatic α-amylase and salivary α-amylase enzymes was studied using starch and yeast α-glucosidase enzyme using 4-nitrophenyl α-d-glucopyranoside as substrate. Further, 2,2-diphenyl-1-picrylhydrazyl radical scavenging and reactive oxygen species inhibition assay was performed against AFE.

RESULTS

AFE potently inhibited the activities of α-amylase and α-glucosidase in a concentration-dependent manner with half maximal inhibitory concentration (IC50) values of 135.70 μg mL-1 and 106.70 μg mL-1 respectively. Furthermore, it also showed inhibition of α-glucosidase (IC50 562.9 μg mL-1) and dipeptidyl peptidase-4 (DPP-4; IC50 3770 μg mL-1) enzyme activities. AFE is a potent antioxidant showing a free radical scavenging activity (IC50 2.37 μg mL-1) and protecting against cellular reactive oxygen species (IC50 1.77 μg mL-1), and the effects elicited could be attributed to its phytoconstituents.

CONCLUSION

AFE showed significant gastric acid resistance and was also found to be thermostable against wet heat. Excellent α-amylase, α-glucosidase, and DPP-4 inhibitory activities of AFE, as well as antioxidant activities, strongly recommend its use for the management of type 2 diabetes mellitus. © 2019 The Authors. Journal of The Science of Food and Agriculture published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.

Authors+Show Affiliations

Sami Labs Limited, Bangalore, India. Sabinsa Corporation, East Windsor, NJ, USA. Sabinsa Corporation, Payson, UT, USA.Sami Labs Limited, Bangalore, India. Sabinsa Corporation, East Windsor, NJ, USA. Sabinsa Corporation, Payson, UT, USA.Sami Labs Limited, Bangalore, India.Sabinsa Corporation, East Windsor, NJ, USA.Sami Labs Limited, Bangalore, India.Sami Labs Limited, Bangalore, India.Sami Labs Limited, Bangalore, India.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31487036

Citation

Majeed, Muhammed, et al. "Standardized Emblica Officinalis Fruit Extract Inhibited the Activities of Α-amylase, Α-glucosidase, and Dipeptidyl Peptidase-4 and Displayed Antioxidant Potential." Journal of the Science of Food and Agriculture, vol. 100, no. 2, 2020, pp. 509-516.
Majeed M, Majeed S, Mundkur L, et al. Standardized Emblica officinalis fruit extract inhibited the activities of α-amylase, α-glucosidase, and dipeptidyl peptidase-4 and displayed antioxidant potential. J Sci Food Agric. 2020;100(2):509-516.
Majeed, M., Majeed, S., Mundkur, L., Nagabhushanam, K., Arumugam, S., Beede, K., & Ali, F. (2020). Standardized Emblica officinalis fruit extract inhibited the activities of α-amylase, α-glucosidase, and dipeptidyl peptidase-4 and displayed antioxidant potential. Journal of the Science of Food and Agriculture, 100(2), 509-516. https://doi.org/10.1002/jsfa.10020
Majeed M, et al. Standardized Emblica Officinalis Fruit Extract Inhibited the Activities of Α-amylase, Α-glucosidase, and Dipeptidyl Peptidase-4 and Displayed Antioxidant Potential. J Sci Food Agric. 2020 Jan 30;100(2):509-516. PubMed PMID: 31487036.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Standardized Emblica officinalis fruit extract inhibited the activities of α-amylase, α-glucosidase, and dipeptidyl peptidase-4 and displayed antioxidant potential. AU - Majeed,Muhammed, AU - Majeed,Shaheen, AU - Mundkur,Lakshmi, AU - Nagabhushanam,Kalyanam, AU - Arumugam,Sivakumar, AU - Beede,Kirankumar, AU - Ali,Furqan, Y1 - 2019/11/13/ PY - 2019/05/06/received PY - 2019/08/28/revised PY - 2019/08/30/accepted PY - 2019/9/6/pubmed PY - 2019/12/20/medline PY - 2019/9/6/entrez KW - DPP-4 KW - Saberry® KW - amla fruit extract KW - type 2 diabetes mellitus KW - α-amylase KW - α-glucosidase KW - β-glucogallin SP - 509 EP - 516 JF - Journal of the science of food and agriculture JO - J Sci Food Agric VL - 100 IS - 2 N2 - BACKGROUND: Emblica officinalis, known as amla in Ayurveda, has been used as a folk medicine to treat numerous pathological conditions, including diabetes. However, the novel extract of E. officinalis fruit extract (amla fruit extract, AFE, Saberry®) containing 100 g kg-1 β-glucogallin along with hydrolyzable tannins has not yet been extensively studied for its antidiabetic potential. OBJECTIVE: The aim of this study was to investigate the antidiabetic and antioxidant activities of AFE and its stability during gastric stress as well as its thermostability. METHODS: The effect of AFE on the inhibition of pancreatic α-amylase and salivary α-amylase enzymes was studied using starch and yeast α-glucosidase enzyme using 4-nitrophenyl α-d-glucopyranoside as substrate. Further, 2,2-diphenyl-1-picrylhydrazyl radical scavenging and reactive oxygen species inhibition assay was performed against AFE. RESULTS: AFE potently inhibited the activities of α-amylase and α-glucosidase in a concentration-dependent manner with half maximal inhibitory concentration (IC50) values of 135.70 μg mL-1 and 106.70 μg mL-1 respectively. Furthermore, it also showed inhibition of α-glucosidase (IC50 562.9 μg mL-1) and dipeptidyl peptidase-4 (DPP-4; IC50 3770 μg mL-1) enzyme activities. AFE is a potent antioxidant showing a free radical scavenging activity (IC50 2.37 μg mL-1) and protecting against cellular reactive oxygen species (IC50 1.77 μg mL-1), and the effects elicited could be attributed to its phytoconstituents. CONCLUSION: AFE showed significant gastric acid resistance and was also found to be thermostable against wet heat. Excellent α-amylase, α-glucosidase, and DPP-4 inhibitory activities of AFE, as well as antioxidant activities, strongly recommend its use for the management of type 2 diabetes mellitus. © 2019 The Authors. Journal of The Science of Food and Agriculture published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry. SN - 1097-0010 UR - https://www.unboundmedicine.com/medline/citation/31487036/Standardized_Emblica_officinalis_fruit_extract_inhibited_the_activities_of_α_amylase_α_glucosidase_and_dipeptidyl_peptidase_4_and_displayed_antioxidant_potential_ L2 - https://doi.org/10.1002/jsfa.10020 DB - PRIME DP - Unbound Medicine ER -