Tags

Type your tag names separated by a space and hit enter

Interleukin-5 levels are decreased in the plasma of coronary artery disease patients and inhibit Th1 and Th17 differentiation in vitro.

Abstract

INTRODUCTION AND OBJECTIVES

Interleukin (IL)-5 is an anti-inflammatory cytokine that has been demonstrated to be involved in cardiovascular diseases, including aortic aneurysm and heart failure. This study aimed to investigate the involvement of IL-5 in coronary artery disease (CAD) and the possible mechanisms.

METHODS

We analyzed IL-5 expression in human coronary artery specimens collected from CAD patients and deceased donors. Plasma IL-5, IL-17, and interferon-γ levels in CAD patients were detected using ELISA kits, with samples from chest pain patients (non-CAD) as controls. Mouse CD4+T helper (Th) cells were separated, and the effect of IL-5 on Th1, regulatory T cell and Th17 differentiation and mRNA levels of their characteristic cytokines were detected using flow cytometry and reverse transcription-quantitative polymerase chain reaction, respectively.

RESULTS

IL-5 was significantly decreased in the coronary plaque of CAD patients compared with the deceased donors group, and IL-5 was mainly derived from macrophages in the coronary artery plaque. Compared with the non-CAD group, plasma IL-5 levels in the CAD groups were significantly lower, and the sequence from high to low was stable angina pectoris, unstable angina pectoris, and acute myocardial infarction. Binary linear regression analysis showed that IL-5 was independently correlated with the occurrence of CAD. Recombinant mouse IL-5 treatment decreased Th1 and Th17 levels and mRNA expression of their characteristic cytokines in oxidized low-density lipoprotein-treated CD4+Th cells.

CONCLUSION

IL-5 levels were decreased in CAD patients and inhibited oxidized low-density lipoprotein Th1 and Th17 differentiation in vitro.

Authors+Show Affiliations

Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, China; Cardiovascular Research Institute, Wuhan University, Wuhan, China; Hubei Key Laboratory of Cardiology, Wuhan, China.Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, China; Cardiovascular Research Institute, Wuhan University, Wuhan, China; Hubei Key Laboratory of Cardiology, Wuhan, China.Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, China; Cardiovascular Research Institute, Wuhan University, Wuhan, China; Hubei Key Laboratory of Cardiology, Wuhan, China.Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, China; Cardiovascular Research Institute, Wuhan University, Wuhan, China; Hubei Key Laboratory of Cardiology, Wuhan, China.Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, China; Cardiovascular Research Institute, Wuhan University, Wuhan, China; Hubei Key Laboratory of Cardiology, Wuhan, China.Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, China; Cardiovascular Research Institute, Wuhan University, Wuhan, China; Hubei Key Laboratory of Cardiology, Wuhan, China.Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, China; Cardiovascular Research Institute, Wuhan University, Wuhan, China; Hubei Key Laboratory of Cardiology, Wuhan, China.Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, China; Cardiovascular Research Institute, Wuhan University, Wuhan, China; Hubei Key Laboratory of Cardiology, Wuhan, China.Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, China; Cardiovascular Research Institute, Wuhan University, Wuhan, China; Hubei Key Laboratory of Cardiology, Wuhan, China. Electronic address: wanjun@whu.edu.cn.

Pub Type(s)

Journal Article

Language

eng spa

PubMed ID

31495774

Citation

Ye, Di, et al. "Interleukin-5 Levels Are Decreased in the Plasma of Coronary Artery Disease Patients and Inhibit Th1 and Th17 Differentiation in Vitro." Revista Espanola De Cardiologia (English Ed.), 2019.
Ye D, Wang Z, Ye J, et al. Interleukin-5 levels are decreased in the plasma of coronary artery disease patients and inhibit Th1 and Th17 differentiation in vitro. Rev Esp Cardiol (Engl Ed). 2019.
Ye, D., Wang, Z., Ye, J., Wang, M., Liu, J., Xu, Y., ... Wan, J. (2019). Interleukin-5 levels are decreased in the plasma of coronary artery disease patients and inhibit Th1 and Th17 differentiation in vitro. Revista Espanola De Cardiologia (English Ed.), doi:10.1016/j.rec.2019.07.005.
Ye D, et al. Interleukin-5 Levels Are Decreased in the Plasma of Coronary Artery Disease Patients and Inhibit Th1 and Th17 Differentiation in Vitro. Rev Esp Cardiol (Engl Ed). 2019 Sep 5; PubMed PMID: 31495774.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Interleukin-5 levels are decreased in the plasma of coronary artery disease patients and inhibit Th1 and Th17 differentiation in vitro. AU - Ye,Di, AU - Wang,Zhen, AU - Ye,Jing, AU - Wang,Menglong, AU - Liu,Jianfang, AU - Xu,Yao, AU - Jiang,Huimin, AU - Chen,Jiangbin, AU - Wan,Jun, Y1 - 2019/09/05/ PY - 2019/01/05/received PY - 2019/07/12/accepted PY - 2019/9/10/entrez KW - Coronary artery disease KW - Célula T reguladora KW - Diferenciación de Th1 KW - Diferenciación de Th17 KW - Enfermedad coronaria KW - Inflamación KW - Inflammation KW - Interleucina 5 KW - Interleukin-5 KW - Regulatory T cell KW - Th1 differentiation KW - Th17 differentiation JF - Revista espanola de cardiologia (English ed.) JO - Rev Esp Cardiol (Engl Ed) N2 - INTRODUCTION AND OBJECTIVES: Interleukin (IL)-5 is an anti-inflammatory cytokine that has been demonstrated to be involved in cardiovascular diseases, including aortic aneurysm and heart failure. This study aimed to investigate the involvement of IL-5 in coronary artery disease (CAD) and the possible mechanisms. METHODS: We analyzed IL-5 expression in human coronary artery specimens collected from CAD patients and deceased donors. Plasma IL-5, IL-17, and interferon-γ levels in CAD patients were detected using ELISA kits, with samples from chest pain patients (non-CAD) as controls. Mouse CD4+T helper (Th) cells were separated, and the effect of IL-5 on Th1, regulatory T cell and Th17 differentiation and mRNA levels of their characteristic cytokines were detected using flow cytometry and reverse transcription-quantitative polymerase chain reaction, respectively. RESULTS: IL-5 was significantly decreased in the coronary plaque of CAD patients compared with the deceased donors group, and IL-5 was mainly derived from macrophages in the coronary artery plaque. Compared with the non-CAD group, plasma IL-5 levels in the CAD groups were significantly lower, and the sequence from high to low was stable angina pectoris, unstable angina pectoris, and acute myocardial infarction. Binary linear regression analysis showed that IL-5 was independently correlated with the occurrence of CAD. Recombinant mouse IL-5 treatment decreased Th1 and Th17 levels and mRNA expression of their characteristic cytokines in oxidized low-density lipoprotein-treated CD4+Th cells. CONCLUSION: IL-5 levels were decreased in CAD patients and inhibited oxidized low-density lipoprotein Th1 and Th17 differentiation in vitro. SN - 1885-5857 UR - https://www.unboundmedicine.com/medline/citation/31495774/Interleukin-5_levels_are_decreased_in_the_plasma_of_coronary_artery_disease_patients_and_inhibit_Th1_and_Th17_differentiation_in_vitro L2 - http://www.revespcardiol.org/en/linksolver/ft/pii/S1885-5857(19)30218-X DB - PRIME DP - Unbound Medicine ER -