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tRNA-derived fragment tRF-03357 promotes cell proliferation, migration and invasion in high-grade serous ovarian cancer.
Onco Targets Ther 2019; 12:6371-6383OT

Abstract

Background

High-grade serous ovarian cancer (HGSOC) is one of the most common ovarian epithelial malignancies. tRNA-derived fragments (tRFs) have been identified as novel potential biomarkers and targets for cancer therapy. Nevertheless, the influence of tRFs on HGSOC remains unknown. This study aimed to identify HGSOC-associated tRFs and to investigate the function and mechanism of key tRFs in SK-OV-3 ovarian cancer cells.

Methods

The tRF profiles in HGSOC patients and controls were investigated using small RNA sequencing. Differentially expressed tRFs were verified by real-time PCR, and a key tRF was evaluated in a function study.

Results

A total of 27 tRFs were differentially expressed between HGSOC patients and controls. Differentially expressed tRFs were mainly involved in the functions of protein phosphorylation, transcription and cell migration and the pathway of cancer, and the MAPK and Wnt signaling pathways. Real-time PCR verified that tRF-03357 and tRF-03358 were significantly increased in the HGSOC serum samples and SK-OV-3 cells compared to their expression levels in the controls. Importantly, tRF-03357 promoted SK-OV-3 cell proliferation, migration and invasion. Moreover, tRF-03357 was predictively targeted, and significantly downregulated HMBOX1.

Conclusion

This study suggests that tRF-03357 might promote cell proliferation, migration and invasion, partly by modulating HMBOX1 in HGSOC.

Authors+Show Affiliations

Department of Obstetrics and Gynecology, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui 230022, People's Republic of China. Anhui Province Key Laboratory of Reproductive Health and Genetics, Biopreservation and Artificial Organs, Anhui Provincial Engineering Research Center, Anhui Medical University, Hefei, People's Republic of China.School of Basic Medicine, Anhui Medical University, Hefei, People's Republic of China.Department of Obstetrics and Gynecology, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui 230022, People's Republic of China.Department of Obstetrics and Gynecology, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui 230022, People's Republic of China.Department of Obstetrics and Gynecology, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui 230022, People's Republic of China.Department of Obstetrics and Gynecology, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui 230022, People's Republic of China.Department of Obstetrics and Gynecology, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui 230022, People's Republic of China. Anhui Province Key Laboratory of Reproductive Health and Genetics, Biopreservation and Artificial Organs, Anhui Provincial Engineering Research Center, Anhui Medical University, Hefei, People's Republic of China. Reproductive Medicine Center, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui 230022, People's Republic of China.Department of Obstetrics and Gynecology, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui 230022, People's Republic of China. Anhui Province Key Laboratory of Reproductive Health and Genetics, Biopreservation and Artificial Organs, Anhui Provincial Engineering Research Center, Anhui Medical University, Hefei, People's Republic of China. Reproductive Medicine Center, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui 230022, People's Republic of China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31496739

Citation

Zhang, Minmin, et al. "TRNA-derived Fragment tRF-03357 Promotes Cell Proliferation, Migration and Invasion in High-grade Serous Ovarian Cancer." OncoTargets and Therapy, vol. 12, 2019, pp. 6371-6383.
Zhang M, Li F, Wang J, et al. TRNA-derived fragment tRF-03357 promotes cell proliferation, migration and invasion in high-grade serous ovarian cancer. Onco Targets Ther. 2019;12:6371-6383.
Zhang, M., Li, F., Wang, J., He, W., Li, Y., Li, H., ... Cao, Y. (2019). TRNA-derived fragment tRF-03357 promotes cell proliferation, migration and invasion in high-grade serous ovarian cancer. OncoTargets and Therapy, 12, pp. 6371-6383. doi:10.2147/OTT.S206861.
Zhang M, et al. TRNA-derived Fragment tRF-03357 Promotes Cell Proliferation, Migration and Invasion in High-grade Serous Ovarian Cancer. Onco Targets Ther. 2019;12:6371-6383. PubMed PMID: 31496739.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - tRNA-derived fragment tRF-03357 promotes cell proliferation, migration and invasion in high-grade serous ovarian cancer. AU - Zhang,Minmin, AU - Li,Feifei, AU - Wang,Jing, AU - He,Wenzhu, AU - Li,Yun, AU - Li,Hongyan, AU - Wei,Zhaolian, AU - Cao,Yunxia, Y1 - 2019/08/16/ PY - 2019/02/26/received PY - 2019/07/16/accepted PY - 2019/9/10/entrez PY - 2019/9/10/pubmed PY - 2019/9/10/medline KW - cell growth KW - high-grade serous ovarian cancer KW - invasion KW - migration KW - tRNA-derived fragments SP - 6371 EP - 6383 JF - OncoTargets and therapy JO - Onco Targets Ther VL - 12 N2 - Background: High-grade serous ovarian cancer (HGSOC) is one of the most common ovarian epithelial malignancies. tRNA-derived fragments (tRFs) have been identified as novel potential biomarkers and targets for cancer therapy. Nevertheless, the influence of tRFs on HGSOC remains unknown. This study aimed to identify HGSOC-associated tRFs and to investigate the function and mechanism of key tRFs in SK-OV-3 ovarian cancer cells. Methods: The tRF profiles in HGSOC patients and controls were investigated using small RNA sequencing. Differentially expressed tRFs were verified by real-time PCR, and a key tRF was evaluated in a function study. Results: A total of 27 tRFs were differentially expressed between HGSOC patients and controls. Differentially expressed tRFs were mainly involved in the functions of protein phosphorylation, transcription and cell migration and the pathway of cancer, and the MAPK and Wnt signaling pathways. Real-time PCR verified that tRF-03357 and tRF-03358 were significantly increased in the HGSOC serum samples and SK-OV-3 cells compared to their expression levels in the controls. Importantly, tRF-03357 promoted SK-OV-3 cell proliferation, migration and invasion. Moreover, tRF-03357 was predictively targeted, and significantly downregulated HMBOX1. Conclusion: This study suggests that tRF-03357 might promote cell proliferation, migration and invasion, partly by modulating HMBOX1 in HGSOC. SN - 1178-6930 UR - https://www.unboundmedicine.com/medline/citation/31496739/tRNA_derived_fragment_tRF_03357_promotes_cell_proliferation_migration_and_invasion_in_high_grade_serous_ovarian_cancer_ L2 - https://dx.doi.org/10.2147/OTT.S206861 DB - PRIME DP - Unbound Medicine ER -