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Simultaneous Quantitative Determination of Amphetamines, Opiates, Ketamine, Cocaine and Metabolites in Human Hair: Application to Forensic Cases of Drug Abuse.
J Forensic Sci. 2020 Mar; 65(2):563-569.JF

Abstract

A method using liquid chromatography-tandem mass spectrometry (LC-MS/MS) to simultaneously quantify amphetamines, opiates, ketamine, cocaine, and metabolites in human hair is described. Hair samples (50 mg) were extracted with methanol utilizing cryogenic grinding. Calibration curves for all the analytes were established in the concentration range 0.05-10 ng/mg. The recoveries were above 72%, except for AMP at the limit of quantification (LOQ), which was 48%. The accuracies were within ±20% at the LOQ (0.05 ng/mg) and between -11% and 13.3% at 0.3 and 9.5 ng/mg, respectively. The intraday and interday precisions were within 19.6% and 19.8%, respectively. A proficiency test was applied to the validated method with z-scores within ±2, demonstrating the accuracy of the method for the determination of drugs of abuse in the hair of individuals suspected of abusing drugs. The hair concentration ranges, means, and medians are summarized for abused drugs in 158 authentic cases.

Authors+Show Affiliations

Institute of Forensic Science, Guizhou Medical University, Guiyang, China. Department of Forensic Toxicology, Shanghai Key Laboratory of Forensic Medicine, Shanghai Forensic Science Platform, Academy of Forensic Science, Shanghai, China.Department of Forensic Toxicology, Shanghai Key Laboratory of Forensic Medicine, Shanghai Forensic Science Platform, Academy of Forensic Science, Shanghai, China.Institute of Forensic Science, Guizhou Medical University, Guiyang, China.Department of Forensic Toxicology, Shanghai Key Laboratory of Forensic Medicine, Shanghai Forensic Science Platform, Academy of Forensic Science, Shanghai, China.Department of Forensic Toxicology, Shanghai Key Laboratory of Forensic Medicine, Shanghai Forensic Science Platform, Academy of Forensic Science, Shanghai, China.Department of Forensic Toxicology, Shanghai Key Laboratory of Forensic Medicine, Shanghai Forensic Science Platform, Academy of Forensic Science, Shanghai, China.Department of Forensic Toxicology, Shanghai Key Laboratory of Forensic Medicine, Shanghai Forensic Science Platform, Academy of Forensic Science, Shanghai, China.Department of Forensic Toxicology, Shanghai Key Laboratory of Forensic Medicine, Shanghai Forensic Science Platform, Academy of Forensic Science, Shanghai, China.Department of Forensic Toxicology, Shanghai Key Laboratory of Forensic Medicine, Shanghai Forensic Science Platform, Academy of Forensic Science, Shanghai, China.

Pub Type(s)

Journal Article
Validation Study

Language

eng

PubMed ID

31498435

Citation

Zhuo, Yue, et al. "Simultaneous Quantitative Determination of Amphetamines, Opiates, Ketamine, Cocaine and Metabolites in Human Hair: Application to Forensic Cases of Drug Abuse." Journal of Forensic Sciences, vol. 65, no. 2, 2020, pp. 563-569.
Zhuo Y, Wang X, Wu J, et al. Simultaneous Quantitative Determination of Amphetamines, Opiates, Ketamine, Cocaine and Metabolites in Human Hair: Application to Forensic Cases of Drug Abuse. J Forensic Sci. 2020;65(2):563-569.
Zhuo, Y., Wang, X., Wu, J., Zhang, S., Deng, H., Liu, W., Wu, H., Xiang, P., & Shen, M. (2020). Simultaneous Quantitative Determination of Amphetamines, Opiates, Ketamine, Cocaine and Metabolites in Human Hair: Application to Forensic Cases of Drug Abuse. Journal of Forensic Sciences, 65(2), 563-569. https://doi.org/10.1111/1556-4029.14179
Zhuo Y, et al. Simultaneous Quantitative Determination of Amphetamines, Opiates, Ketamine, Cocaine and Metabolites in Human Hair: Application to Forensic Cases of Drug Abuse. J Forensic Sci. 2020;65(2):563-569. PubMed PMID: 31498435.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Simultaneous Quantitative Determination of Amphetamines, Opiates, Ketamine, Cocaine and Metabolites in Human Hair: Application to Forensic Cases of Drug Abuse. AU - Zhuo,Yue, AU - Wang,Xin, AU - Wu,Jingjie, AU - Zhang,Sujing, AU - Deng,Hongxiao, AU - Liu,Wei, AU - Wu,Hejian, AU - Xiang,Ping, AU - Shen,Min, Y1 - 2019/09/09/ PY - 2019/06/18/received PY - 2019/07/07/revised PY - 2019/08/09/revised PY - 2019/08/14/accepted PY - 2019/9/10/pubmed PY - 2020/3/12/medline PY - 2019/9/10/entrez KW - amphetamines KW - cocaine KW - forensic science KW - forensic toxicology KW - hair analysis KW - ketamine KW - liquid chromatography-tandem mass spectrometry KW - metabolite-to-drug concentration ratio KW - opiates SP - 563 EP - 569 JF - Journal of forensic sciences JO - J Forensic Sci VL - 65 IS - 2 N2 - A method using liquid chromatography-tandem mass spectrometry (LC-MS/MS) to simultaneously quantify amphetamines, opiates, ketamine, cocaine, and metabolites in human hair is described. Hair samples (50 mg) were extracted with methanol utilizing cryogenic grinding. Calibration curves for all the analytes were established in the concentration range 0.05-10 ng/mg. The recoveries were above 72%, except for AMP at the limit of quantification (LOQ), which was 48%. The accuracies were within ±20% at the LOQ (0.05 ng/mg) and between -11% and 13.3% at 0.3 and 9.5 ng/mg, respectively. The intraday and interday precisions were within 19.6% and 19.8%, respectively. A proficiency test was applied to the validated method with z-scores within ±2, demonstrating the accuracy of the method for the determination of drugs of abuse in the hair of individuals suspected of abusing drugs. The hair concentration ranges, means, and medians are summarized for abused drugs in 158 authentic cases. SN - 1556-4029 UR - https://www.unboundmedicine.com/medline/citation/31498435/Simultaneous_Quantitative_Determination_of_Amphetamines_Opiates_Ketamine_Cocaine_and_Metabolites_in_Human_Hair:_Application_to_Forensic_Cases_of_Drug_Abuse_ DB - PRIME DP - Unbound Medicine ER -