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Improvement of vitamin B12 status with Spirulina supplementation in Wistar rats validated through functional and circulatory markers.
J Food Biochem. 2019 11; 43(11):e13038.JF

Abstract

Spirulina evaluated as a source of vitamin B12 through the modulation of vitamin B12 deficiency mediated physiological and biochemical changes in experimental animals. The B12 deficient male weanling Wistar rats were fed with Spirulina-supplemented diet for 10 weeks. An increase in urinary methylmalonic acid (22.70 ± 4.08 µmol/moles of creatinine) and plasma homocysteine (16.55 ± 0.48 µmol/L) levels in the B12 deficient group was observed, while these were equal to control in the Spirulina fed group (8.71 ± 0.48 µmol/mol of creatinine and 6.88 ± 1.18 µmol/L, respectively). The vitamin B12 levels in serum (874.27 ± 89.69), plasma (615.53 ± 26.5 pg/ml), kidney (10.19 ± 1.066 ng/g), and liver tissues (6.37 ± 0.62 ng/g) in the Spirulina fed group were similar to control. Severe atrophic changes in the testes and altered tissue architecture in lung and spleen as seen in the B12 deficient group were normalized in the Spirulina fed group. The study validates that Spirulina can improve the vitamin B12 status. PRACTICAL APPLICATIONS: The present study showed that the supplementation of Spirulina in the diet of vitamin B12 deficient rats leads to the normalization of vitamin B12 deficiency-induced circulatory and functional biomarkers along with biochemical and histological changes. Vegetarian sources for vitamin B12 are limited and the results presented here provide scientific validation for the use of Spirulina as a potential vegetarian source of bioavailable vitamin B12 .

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Publisher Full Text (DOI)

Authors+Show Affiliations

Madhubalaji CK
Plant Cell Biotechnology Department, CSIR - Central Food Technological Research Institute, Mysuru, India. Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, India.
Rashmi V
Plant Cell Biotechnology Department, CSIR - Central Food Technological Research Institute, Mysuru, India.
Chauhan VS
Plant Cell Biotechnology Department, CSIR - Central Food Technological Research Institute, Mysuru, India. Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, India.
Shylaja MD
Department of Biochemistry, CSIR - Central Food Technological Research Institute, Mysuru, India.
Sarada R
Plant Cell Biotechnology Department, CSIR - Central Food Technological Research Institute, Mysuru, India. Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, India.

MeSH

AnimalsBiomarkersDietary SupplementsHumansMaleRatsRats, WistarSpirulinaVitamin B 12Vitamin B 12 Deficiency

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

31502254

Citation

Madhubalaji, Chegu Krishnamurthi, et al. "Improvement of Vitamin B12 Status With Spirulina Supplementation in Wistar Rats Validated Through Functional and Circulatory Markers." Journal of Food Biochemistry, vol. 43, no. 11, 2019, pp. e13038.
Madhubalaji CK, Rashmi V, Chauhan VS, et al. Improvement of vitamin B12 status with Spirulina supplementation in Wistar rats validated through functional and circulatory markers. J Food Biochem. 2019;43(11):e13038.
Madhubalaji, C. K., Rashmi, V., Chauhan, V. S., Shylaja, M. D., & Sarada, R. (2019). Improvement of vitamin B12 status with Spirulina supplementation in Wistar rats validated through functional and circulatory markers. Journal of Food Biochemistry, 43(11), e13038. https://doi.org/10.1111/jfbc.13038
Madhubalaji CK, et al. Improvement of Vitamin B12 Status With Spirulina Supplementation in Wistar Rats Validated Through Functional and Circulatory Markers. J Food Biochem. 2019;43(11):e13038. PubMed PMID: 31502254.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Improvement of vitamin B12 status with Spirulina supplementation in Wistar rats validated through functional and circulatory markers. AU - Madhubalaji,Chegu Krishnamurthi, AU - Rashmi,Venkatasubbaiah, AU - Chauhan,Vikas Singh, AU - Shylaja,M Dharmesh, AU - Sarada,Ravi, Y1 - 2019/09/09/ PY - 2019/03/18/received PY - 2019/08/16/revised PY - 2019/08/19/accepted PY - 2019/9/11/pubmed PY - 2020/9/18/medline PY - 2019/9/11/entrez KW - Spirulina KW - B12 deficiency KW - histology KW - homocysteine KW - methylmalonic acid KW - vitamin B12 SP - e13038 EP - e13038 JF - Journal of food biochemistry JO - J Food Biochem VL - 43 IS - 11 N2 - Spirulina evaluated as a source of vitamin B12 through the modulation of vitamin B12 deficiency mediated physiological and biochemical changes in experimental animals. The B12 deficient male weanling Wistar rats were fed with Spirulina-supplemented diet for 10 weeks. An increase in urinary methylmalonic acid (22.70 ± 4.08 µmol/moles of creatinine) and plasma homocysteine (16.55 ± 0.48 µmol/L) levels in the B12 deficient group was observed, while these were equal to control in the Spirulina fed group (8.71 ± 0.48 µmol/mol of creatinine and 6.88 ± 1.18 µmol/L, respectively). The vitamin B12 levels in serum (874.27 ± 89.69), plasma (615.53 ± 26.5 pg/ml), kidney (10.19 ± 1.066 ng/g), and liver tissues (6.37 ± 0.62 ng/g) in the Spirulina fed group were similar to control. Severe atrophic changes in the testes and altered tissue architecture in lung and spleen as seen in the B12 deficient group were normalized in the Spirulina fed group. The study validates that Spirulina can improve the vitamin B12 status. PRACTICAL APPLICATIONS: The present study showed that the supplementation of Spirulina in the diet of vitamin B12 deficient rats leads to the normalization of vitamin B12 deficiency-induced circulatory and functional biomarkers along with biochemical and histological changes. Vegetarian sources for vitamin B12 are limited and the results presented here provide scientific validation for the use of Spirulina as a potential vegetarian source of bioavailable vitamin B12 . SN - 1745-4514 UR - https://www.unboundmedicine.com/medline/citation/31502254/Improvement_of_vitamin_B12_status_with_Spirulina_supplementation_in_Wistar_rats_validated_through_functional_and_circulatory_markers_ DB - PRIME DP - Unbound Medicine ER -