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Characterization and Quantification of Major Flavonol Glycosides in Ramps (Allium tricoccum).
Molecules. 2019 Sep 09; 24(18)M

Abstract

The ramp (Allium tricoccum) is a traditional plant in the eastern Appalachian Mountains. Ramps have been used in traditional medicine for their health-promoting roles in lowering blood pressure and cholesterol. Information on the chemical composition of the potentially bioactive components in ramps is limited. Therefore, the aim of this work was to characterize and quantify major flavonols in ramps. Flavonoids were extracted in 50% methanol and 3% acetic acid. Characterization was conducted using UHPLC-PDA-MS and MS/MS, and quantification was performed using UHPLC-PDA detection. The major flavonol glycosides were kaempferol sophoroside glucuronide, quercetin sophoroside glucuronide, kaempferol rutinoside glucuronide, quercetin hexoside glucuronide, quercetin sophoroside, and kaempferol sophoroside. All conjugates were detected in leaves. Quercetin and kaempferol sophoroside glucuronide conjugates were detected in the stem, but no flavonol glycosides were detected in the bulb. The total amounts of the identified quercetin and kaempferol conjugates in whole ramps were 0.5972 ± 0.235 and 0.3792 ± 0.130 mg/g dry weight, respectively. Flavonol conjugates were concentrated in the leaves. To our knowledge, this work is the first to identify and quantify the major flavonol glycosides in ramps. Our findings suggest that specifically the leaves may harbor the potentially bioactive flavonols components of the plant.

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Authors+Show Affiliations

Dabeek WM
Division of Animal and Nutritional Sciences, West Virginia University, Morgantown, WV 26506, USA. wmdabeek@mix.wvu.edu.
Kovinich N
Division of Plant and Soil Sciences, West Virginia University, Morgantown, WV 26506, USA. nikola.kovinich@mail.wvu.edu.
Walsh C
Shared Research Facilities, West Virginia University, Morgantown, WV 26506, USA. callee.walsh@mail.wvu.edu.
Ventura Marra M
Division of Animal and Nutritional Sciences, West Virginia University, Morgantown, WV 26506, USA. melissa.marra@mail.wvu.edu.

MeSH

AlliumBlood PressureCholesterolChromatography, High Pressure LiquidFlavonoidsGlucuronidesGlycosidesHumansKaempferolsMedicine, TraditionalPlant LeavesQuercetinTandem Mass Spectrometry

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31505821

Citation

Dabeek, Wijdan M., et al. "Characterization and Quantification of Major Flavonol Glycosides in Ramps (Allium Tricoccum)." Molecules (Basel, Switzerland), vol. 24, no. 18, 2019.
Dabeek WM, Kovinich N, Walsh C, et al. Characterization and Quantification of Major Flavonol Glycosides in Ramps (Allium tricoccum). Molecules. 2019;24(18).
Dabeek, W. M., Kovinich, N., Walsh, C., & Ventura Marra, M. (2019). Characterization and Quantification of Major Flavonol Glycosides in Ramps (Allium tricoccum). Molecules (Basel, Switzerland), 24(18). https://doi.org/10.3390/molecules24183281
Dabeek WM, et al. Characterization and Quantification of Major Flavonol Glycosides in Ramps (Allium Tricoccum). Molecules. 2019 Sep 9;24(18) PubMed PMID: 31505821.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Characterization and Quantification of Major Flavonol Glycosides in Ramps (Allium tricoccum). AU - Dabeek,Wijdan M, AU - Kovinich,Nik, AU - Walsh,Callee, AU - Ventura Marra,Melissa, Y1 - 2019/09/09/ PY - 2019/08/24/received PY - 2019/09/04/revised PY - 2019/09/05/accepted PY - 2019/9/12/entrez PY - 2019/9/12/pubmed PY - 2020/2/1/medline KW - Allium tricoccum KW - flavonols KW - kaempferol KW - quercetin KW - ramps JF - Molecules (Basel, Switzerland) JO - Molecules VL - 24 IS - 18 N2 - The ramp (Allium tricoccum) is a traditional plant in the eastern Appalachian Mountains. Ramps have been used in traditional medicine for their health-promoting roles in lowering blood pressure and cholesterol. Information on the chemical composition of the potentially bioactive components in ramps is limited. Therefore, the aim of this work was to characterize and quantify major flavonols in ramps. Flavonoids were extracted in 50% methanol and 3% acetic acid. Characterization was conducted using UHPLC-PDA-MS and MS/MS, and quantification was performed using UHPLC-PDA detection. The major flavonol glycosides were kaempferol sophoroside glucuronide, quercetin sophoroside glucuronide, kaempferol rutinoside glucuronide, quercetin hexoside glucuronide, quercetin sophoroside, and kaempferol sophoroside. All conjugates were detected in leaves. Quercetin and kaempferol sophoroside glucuronide conjugates were detected in the stem, but no flavonol glycosides were detected in the bulb. The total amounts of the identified quercetin and kaempferol conjugates in whole ramps were 0.5972 ± 0.235 and 0.3792 ± 0.130 mg/g dry weight, respectively. Flavonol conjugates were concentrated in the leaves. To our knowledge, this work is the first to identify and quantify the major flavonol glycosides in ramps. Our findings suggest that specifically the leaves may harbor the potentially bioactive flavonols components of the plant. SN - 1420-3049 UR - https://www.unboundmedicine.com/medline/citation/31505821/Characterization_and_Quantification_of_Major_Flavonol_Glycosides_in_Ramps__Allium_tricoccum__ DB - PRIME DP - Unbound Medicine ER -