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Clonal diversity of Haemophilus influenzae carriage isolated from under the age of 6 years children.
BMC Res Notes 2019; 12(1):565BR

Abstract

OBJECTIVES

Pharyngeal carriers such as H. influenzae seem to constitute the only reservoir and probably the only transmission vehicle of the invasive disease. The aims of this study were to estimate the prevalence of H. influenzae carriage, to characterize antibiotic susceptibility, and to explore genetic diversity of H. influenzae isolates. Sampling was carried out as nasopharynx swabs among children less than 6 years old volunteers. After traditional biochemical tests, isolates were confirmed by targeting omp6 sequence. Following the susceptibility tests, genomic diversity of strains was analyzed by Pulsed-Field Gel Electrophoresis procedure.

RESULTS

Out of 328 nasopharynx swabs, 73 strains were identified as H. influenzae. Among H. influenzae isolates, resistance to chloramphenicol (42%) and ampicillin (43%) was observed. Levofloxacin is the most effective antibiotic and the least effect belonged to tetracycline. By genomic analysis of selected H. influenza, 28 PFGE patterns were achieved among which 11 patterns included at least 2 strains. All strains clustered into 25 different clones. The dendrogram analysis of the isolated H. influenzae strains showed that some of these strains had a clonal relationship and common genetic origin. According to our results, antibiotic resistance didn't show any significant correlation with the clonality of strains.

Authors+Show Affiliations

Department of Microbiology, Pasteur Institute of Iran, Tehran, Iran. Department of Microbiology, Faculty of Biology, Islamic Azad University, North Branch, Tehran, Iran.Department of Microbiology and Virology, School of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran.Department of Microbiology, Pasteur Institute of Iran, Tehran, Iran. mousavi@pasteur.ac.ir.Department of Microbiology, Faculty of Biology, Islamic Azad University, North Branch, Tehran, Iran.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31506105

Citation

Shooraj, Fahimeh, et al. "Clonal Diversity of Haemophilus Influenzae Carriage Isolated From Under the Age of 6 Years Children." BMC Research Notes, vol. 12, no. 1, 2019, p. 565.
Shooraj F, Mirzaei B, Mousavi SF, et al. Clonal diversity of Haemophilus influenzae carriage isolated from under the age of 6 years children. BMC Res Notes. 2019;12(1):565.
Shooraj, F., Mirzaei, B., Mousavi, S. F., & Hosseini, F. (2019). Clonal diversity of Haemophilus influenzae carriage isolated from under the age of 6 years children. BMC Research Notes, 12(1), p. 565. doi:10.1186/s13104-019-4603-7.
Shooraj F, et al. Clonal Diversity of Haemophilus Influenzae Carriage Isolated From Under the Age of 6 Years Children. BMC Res Notes. 2019 Sep 11;12(1):565. PubMed PMID: 31506105.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Clonal diversity of Haemophilus influenzae carriage isolated from under the age of 6 years children. AU - Shooraj,Fahimeh, AU - Mirzaei,Bahman, AU - Mousavi,Seyed Fazlollah, AU - Hosseini,Farzaneh, Y1 - 2019/09/11/ PY - 2019/08/10/received PY - 2019/09/03/accepted PY - 2019/9/12/entrez PY - 2019/9/12/pubmed PY - 2019/9/12/medline KW - Antimicrobial resistance KW - Genomic analysis KW - Hemophilus influenzae KW - PFGE SP - 565 EP - 565 JF - BMC research notes JO - BMC Res Notes VL - 12 IS - 1 N2 - OBJECTIVES: Pharyngeal carriers such as H. influenzae seem to constitute the only reservoir and probably the only transmission vehicle of the invasive disease. The aims of this study were to estimate the prevalence of H. influenzae carriage, to characterize antibiotic susceptibility, and to explore genetic diversity of H. influenzae isolates. Sampling was carried out as nasopharynx swabs among children less than 6 years old volunteers. After traditional biochemical tests, isolates were confirmed by targeting omp6 sequence. Following the susceptibility tests, genomic diversity of strains was analyzed by Pulsed-Field Gel Electrophoresis procedure. RESULTS: Out of 328 nasopharynx swabs, 73 strains were identified as H. influenzae. Among H. influenzae isolates, resistance to chloramphenicol (42%) and ampicillin (43%) was observed. Levofloxacin is the most effective antibiotic and the least effect belonged to tetracycline. By genomic analysis of selected H. influenza, 28 PFGE patterns were achieved among which 11 patterns included at least 2 strains. All strains clustered into 25 different clones. The dendrogram analysis of the isolated H. influenzae strains showed that some of these strains had a clonal relationship and common genetic origin. According to our results, antibiotic resistance didn't show any significant correlation with the clonality of strains. SN - 1756-0500 UR - https://www.unboundmedicine.com/medline/citation/31506105/Clonal_diversity_of_Haemophilus_influenzae_carriage_isolated_from_under_the_age_of_6_years_children L2 - https://bmcresnotes.biomedcentral.com/articles/10.1186/s13104-019-4603-7 DB - PRIME DP - Unbound Medicine ER -