Tags

Type your tag names separated by a space and hit enter

Diversification of giant and large eukaryotic dsDNA viruses predated the origin of modern eukaryotes.
Proc Natl Acad Sci U S A. 2019 09 24; 116(39):19585-19592.PN

Abstract

Giant and large eukaryotic double-stranded DNA viruses from the Nucleo-Cytoplasmic Large DNA Virus (NCLDV) assemblage represent a remarkably diverse and potentially ancient component of the eukaryotic virome. However, their origin(s), evolution, and potential roles in the emergence of modern eukaryotes remain subjects of intense debate. Here we present robust phylogenetic trees of NCLDVs, based on the 8 most conserved proteins responsible for virion morphogenesis and informational processes. Our results uncover the evolutionary relationships between different NCLDV families and support the existence of 2 superclades of NCLDVs, each encompassing several families. We present evidence strongly suggesting that the NCLDV core genes, which are involved in both informational processes and virion formation, were acquired vertically from a common ancestor. Among them, the largest subunits of the DNA-dependent RNA polymerase were transferred between 2 clades of NCLDVs and proto-eukaryotes, giving rise to 2 of the 3 eukaryotic DNA-dependent RNA polymerases. Our results strongly suggest that these transfers and the diversification of NCLDVs predated the emergence of modern eukaryotes, emphasizing the major role of viruses in the evolution of cellular domains.

Authors+Show Affiliations

HUB Bioinformatique et Biostatistique, C3BI, USR 3756 IP CNRS, Institut Pasteur, 75015 Paris, France.Unité de Biologie Moléculaire du Gène ches les Extrêmophiles, Département de Microbiologie, Institut Pasteur, 75015 Paris, France.Unité de Biologie Moléculaire du Gène ches les Extrêmophiles, Département de Microbiologie, Institut Pasteur, 75015 Paris, France.Unité de Biologie Moléculaire du Gène ches les Extrêmophiles, Département de Microbiologie, Institut Pasteur, 75015 Paris, France; patrick.forterre@pasteur.fr morgan.gaia@pasteur.fr. Institute for Integrative Biology of the Cell (I2BC), CEA, CNRS, Université Paris-Sud, Université Paris-Saclay, 91198 Gif-sur-Yvette cedex, France.Unité de Biologie Moléculaire du Gène ches les Extrêmophiles, Département de Microbiologie, Institut Pasteur, 75015 Paris, France; patrick.forterre@pasteur.fr morgan.gaia@pasteur.fr. Institute for Integrative Biology of the Cell (I2BC), CEA, CNRS, Université Paris-Sud, Université Paris-Saclay, 91198 Gif-sur-Yvette cedex, France.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

31506349

Citation

Guglielmini, Julien, et al. "Diversification of Giant and Large Eukaryotic dsDNA Viruses Predated the Origin of Modern Eukaryotes." Proceedings of the National Academy of Sciences of the United States of America, vol. 116, no. 39, 2019, pp. 19585-19592.
Guglielmini J, Woo AC, Krupovic M, et al. Diversification of giant and large eukaryotic dsDNA viruses predated the origin of modern eukaryotes. Proc Natl Acad Sci U S A. 2019;116(39):19585-19592.
Guglielmini, J., Woo, A. C., Krupovic, M., Forterre, P., & Gaia, M. (2019). Diversification of giant and large eukaryotic dsDNA viruses predated the origin of modern eukaryotes. Proceedings of the National Academy of Sciences of the United States of America, 116(39), 19585-19592. https://doi.org/10.1073/pnas.1912006116
Guglielmini J, et al. Diversification of Giant and Large Eukaryotic dsDNA Viruses Predated the Origin of Modern Eukaryotes. Proc Natl Acad Sci U S A. 2019 09 24;116(39):19585-19592. PubMed PMID: 31506349.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Diversification of giant and large eukaryotic dsDNA viruses predated the origin of modern eukaryotes. AU - Guglielmini,Julien, AU - Woo,Anthony C, AU - Krupovic,Mart, AU - Forterre,Patrick, AU - Gaia,Morgan, Y1 - 2019/09/10/ PY - 2019/9/12/pubmed PY - 2020/4/22/medline PY - 2019/9/12/entrez KW - NCLDV KW - RNA polymerase KW - evolution KW - giant viruses KW - proto-eukaryotes SP - 19585 EP - 19592 JF - Proceedings of the National Academy of Sciences of the United States of America JO - Proc Natl Acad Sci U S A VL - 116 IS - 39 N2 - Giant and large eukaryotic double-stranded DNA viruses from the Nucleo-Cytoplasmic Large DNA Virus (NCLDV) assemblage represent a remarkably diverse and potentially ancient component of the eukaryotic virome. However, their origin(s), evolution, and potential roles in the emergence of modern eukaryotes remain subjects of intense debate. Here we present robust phylogenetic trees of NCLDVs, based on the 8 most conserved proteins responsible for virion morphogenesis and informational processes. Our results uncover the evolutionary relationships between different NCLDV families and support the existence of 2 superclades of NCLDVs, each encompassing several families. We present evidence strongly suggesting that the NCLDV core genes, which are involved in both informational processes and virion formation, were acquired vertically from a common ancestor. Among them, the largest subunits of the DNA-dependent RNA polymerase were transferred between 2 clades of NCLDVs and proto-eukaryotes, giving rise to 2 of the 3 eukaryotic DNA-dependent RNA polymerases. Our results strongly suggest that these transfers and the diversification of NCLDVs predated the emergence of modern eukaryotes, emphasizing the major role of viruses in the evolution of cellular domains. SN - 1091-6490 UR - https://www.unboundmedicine.com/medline/citation/31506349/Diversification_of_giant_and_large_eukaryotic_dsDNA_viruses_predated_the_origin_of_modern_eukaryotes_ L2 - http://www.pnas.org/cgi/pmidlookup?view=long&pmid=31506349 DB - PRIME DP - Unbound Medicine ER -