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Antioxidant Activity of an Aqueous Leaf Extract from Uncaria tomentosa and Its Major Alkaloids Mitraphylline and Isomitraphylline in Caenorhabditis elegans.
Molecules 2019; 24(18)M

Abstract

Uncaria tomentosa (Rubiaceae) has a recognized therapeutic potential against various diseases associated with oxidative stress. The aim of this research was to evaluate the antioxidant potential of an aqueous leaf extract (ALE) from U. tomentosa, and its major alkaloids mitraphylline and isomitraphylline. The antioxidant activity of ALE was investigated in vitro using standard assays (DPPH, ABTS and FRAP), while the in vivo activity and mode of action were studied using Caenorhabditis elegans as a model organism. The purified alkaloids did not exhibit antioxidant effects in vivo. ALE reduced the accumulation of reactive oxygen species (ROS) in wild-type worms, and was able to rescue the worms from a lethal dose of the pro-oxidant juglone. The ALE treatment led to a decreased expression of the oxidative stress response related genes sod-3, gst-4, and hsp-16.2. The treatment of mutant worms lacking the DAF-16 transcription factor with ALE resulted in a significant reduction of ROS levels. Contrarily, the extract had a pro-oxidant effect in the worms lacking the SKN-1 transcription factor. Our results suggest that the antioxidant activity of ALE in C. elegans is independent of its alkaloid content, and that SKN-1 is required for ALE-mediated stress resistance.

Authors+Show Affiliations

Departamento de Biotecnologia em Plantas Medicinais, Universidade de Ribeirão Preto, Ribeirão Preto 14096-900, São Paulo, Brazil. bruna@cestari.eu. Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto 14049-900, São Paulo, Brazil. bruna@cestari.eu. Institute of Pharmacy and Molecular Biotechnology, Heidelberg University, D-69120 Heidelberg, Germany. bruna@cestari.eu.Institute of Pharmacy and Molecular Biotechnology, Heidelberg University, D-69120 Heidelberg, Germany. marianaroxocorreia@gmail.com.Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto 14049-900, São Paulo, Brazil. marcosborges@fmrp.usp.br.Institute of Pharmacy and Molecular Biotechnology, Heidelberg University, D-69120 Heidelberg, Germany. hspeixoto1@gmail.com.Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto, Departamento de Química, Universidade de São Paulo, Ribeirão Preto 14049-900, São Paulo, Brazil.Departamento de Biotecnologia em Plantas Medicinais, Universidade de Ribeirão Preto, Ribeirão Preto 14096-900, São Paulo, Brazil. bbertoni@unaerp.br.Departamento de Biotecnologia em Plantas Medicinais, Universidade de Ribeirão Preto, Ribeirão Preto 14096-900, São Paulo, Brazil. siltaleb@gmail.com.Departamento de Biotecnologia em Plantas Medicinais, Universidade de Ribeirão Preto, Ribeirão Preto 14096-900, São Paulo, Brazil. adrianalps@yahoo.com.br.Departamento de Biotecnologia em Plantas Medicinais, Universidade de Ribeirão Preto, Ribeirão Preto 14096-900, São Paulo, Brazil. sfranca@unaerp.br.Departamento de Biotecnologia em Plantas Medicinais, Universidade de Ribeirão Preto, Ribeirão Preto 14096-900, São Paulo, Brazil. apereira@unaerp.br.Institute of Pharmacy and Molecular Biotechnology, Heidelberg University, D-69120 Heidelberg, Germany. wink@uni-heidelberg.de.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31510078

Citation

C Azevedo, Bruna, et al. "Antioxidant Activity of an Aqueous Leaf Extract From Uncaria Tomentosa and Its Major Alkaloids Mitraphylline and Isomitraphylline in Caenorhabditis Elegans." Molecules (Basel, Switzerland), vol. 24, no. 18, 2019.
C Azevedo B, Roxo M, C Borges M, et al. Antioxidant Activity of an Aqueous Leaf Extract from Uncaria tomentosa and Its Major Alkaloids Mitraphylline and Isomitraphylline in Caenorhabditis elegans. Molecules. 2019;24(18).
C Azevedo, B., Roxo, M., C Borges, M., Peixoto, H., Crevelin, E. J., W Bertoni, B., ... Wink, M. (2019). Antioxidant Activity of an Aqueous Leaf Extract from Uncaria tomentosa and Its Major Alkaloids Mitraphylline and Isomitraphylline in Caenorhabditis elegans. Molecules (Basel, Switzerland), 24(18), doi:10.3390/molecules24183299.
C Azevedo B, et al. Antioxidant Activity of an Aqueous Leaf Extract From Uncaria Tomentosa and Its Major Alkaloids Mitraphylline and Isomitraphylline in Caenorhabditis Elegans. Molecules. 2019 Sep 10;24(18) PubMed PMID: 31510078.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antioxidant Activity of an Aqueous Leaf Extract from Uncaria tomentosa and Its Major Alkaloids Mitraphylline and Isomitraphylline in Caenorhabditis elegans. AU - C Azevedo,Bruna, AU - Roxo,Mariana, AU - C Borges,Marcos, AU - Peixoto,Herbenya, AU - Crevelin,Eduardo J, AU - W Bertoni,Bianca, AU - H T Contini,Silvia, AU - Lopes,Adriana A, AU - C França,Suzelei, AU - S Pereira,Ana M, AU - Wink,Michael, Y1 - 2019/09/10/ PY - 2019/08/07/received PY - 2019/08/31/revised PY - 2019/09/04/accepted PY - 2019/9/13/entrez KW - Caenorhabditis elegans KW - Uncaria tomentosa KW - antioxidants KW - isomitraphylline KW - mitraphylline KW - oxidative stress JF - Molecules (Basel, Switzerland) JO - Molecules VL - 24 IS - 18 N2 - Uncaria tomentosa (Rubiaceae) has a recognized therapeutic potential against various diseases associated with oxidative stress. The aim of this research was to evaluate the antioxidant potential of an aqueous leaf extract (ALE) from U. tomentosa, and its major alkaloids mitraphylline and isomitraphylline. The antioxidant activity of ALE was investigated in vitro using standard assays (DPPH, ABTS and FRAP), while the in vivo activity and mode of action were studied using Caenorhabditis elegans as a model organism. The purified alkaloids did not exhibit antioxidant effects in vivo. ALE reduced the accumulation of reactive oxygen species (ROS) in wild-type worms, and was able to rescue the worms from a lethal dose of the pro-oxidant juglone. The ALE treatment led to a decreased expression of the oxidative stress response related genes sod-3, gst-4, and hsp-16.2. The treatment of mutant worms lacking the DAF-16 transcription factor with ALE resulted in a significant reduction of ROS levels. Contrarily, the extract had a pro-oxidant effect in the worms lacking the SKN-1 transcription factor. Our results suggest that the antioxidant activity of ALE in C. elegans is independent of its alkaloid content, and that SKN-1 is required for ALE-mediated stress resistance. SN - 1420-3049 UR - https://www.unboundmedicine.com/medline/citation/31510078/Antioxidant_Activity_of_an_Aqueous_Leaf_Extract_from_Uncaria_tomentosa_and_Its_Major_Alkaloids_Mitraphylline_and_Isomitraphylline_in_Caenorhabditis_elegans_ L2 - http://www.mdpi.com/resolver?pii=molecules24183299 DB - PRIME DP - Unbound Medicine ER -