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Oligodendrocytes express synaptic proteins that modulate myelin sheath formation.
Nat Commun 2019; 10(1):4125NC

Abstract

Vesicular release from neurons promotes myelin sheath growth on axons. Oligodendrocytes express proteins that allow dendrites to respond to vesicular release at synapses, suggesting that axon-myelin contacts use similar communication mechanisms as synapses to form myelin sheaths. To test this, we used fusion proteins to track synaptic vesicle localization and membrane fusion in zebrafish during developmental myelination and investigated expression and localization of PSD95, a dendritic post-synaptic protein, within oligodendrocytes. Synaptic vesicles accumulate and exocytose at ensheathment sites with variable patterning and most sheaths localize PSD95 with patterning similar to exocytosis site location. Disruption of candidate PDZ-binding transsynaptic adhesion proteins in oligodendrocytes cause variable effects on sheath length and number. One candidate, Cadm1b, localizes to myelin sheaths where both PDZ binding and extracellular adhesion to axons mediate sheath growth. Our work raises the possibility that axon-glial communication contributes to myelin plasticity, providing new targets for mechanistic unraveling of developmental myelination.

Authors+Show Affiliations

University of Colorado School of Medicine, Anschutz Medical Campus, Aurora, CO, 80045, USA.University of Colorado School of Medicine, Anschutz Medical Campus, Aurora, CO, 80045, USA. bruce.appel@cuanschutz.edu.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31511515

Citation

Hughes, Alexandria N., and Bruce Appel. "Oligodendrocytes Express Synaptic Proteins That Modulate Myelin Sheath Formation." Nature Communications, vol. 10, no. 1, 2019, p. 4125.
Hughes AN, Appel B. Oligodendrocytes express synaptic proteins that modulate myelin sheath formation. Nat Commun. 2019;10(1):4125.
Hughes, A. N., & Appel, B. (2019). Oligodendrocytes express synaptic proteins that modulate myelin sheath formation. Nature Communications, 10(1), p. 4125. doi:10.1038/s41467-019-12059-y.
Hughes AN, Appel B. Oligodendrocytes Express Synaptic Proteins That Modulate Myelin Sheath Formation. Nat Commun. 2019 Sep 11;10(1):4125. PubMed PMID: 31511515.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Oligodendrocytes express synaptic proteins that modulate myelin sheath formation. AU - Hughes,Alexandria N, AU - Appel,Bruce, Y1 - 2019/09/11/ PY - 2019/04/26/received PY - 2019/08/14/accepted PY - 2019/9/13/entrez PY - 2019/9/13/pubmed PY - 2019/9/13/medline SP - 4125 EP - 4125 JF - Nature communications JO - Nat Commun VL - 10 IS - 1 N2 - Vesicular release from neurons promotes myelin sheath growth on axons. Oligodendrocytes express proteins that allow dendrites to respond to vesicular release at synapses, suggesting that axon-myelin contacts use similar communication mechanisms as synapses to form myelin sheaths. To test this, we used fusion proteins to track synaptic vesicle localization and membrane fusion in zebrafish during developmental myelination and investigated expression and localization of PSD95, a dendritic post-synaptic protein, within oligodendrocytes. Synaptic vesicles accumulate and exocytose at ensheathment sites with variable patterning and most sheaths localize PSD95 with patterning similar to exocytosis site location. Disruption of candidate PDZ-binding transsynaptic adhesion proteins in oligodendrocytes cause variable effects on sheath length and number. One candidate, Cadm1b, localizes to myelin sheaths where both PDZ binding and extracellular adhesion to axons mediate sheath growth. Our work raises the possibility that axon-glial communication contributes to myelin plasticity, providing new targets for mechanistic unraveling of developmental myelination. SN - 2041-1723 UR - https://www.unboundmedicine.com/medline/citation/31511515/Oligodendrocytes_express_synaptic_proteins_that_modulate_myelin_sheath_formation L2 - http://dx.doi.org/10.1038/s41467-019-12059-y DB - PRIME DP - Unbound Medicine ER -
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