Tags

Type your tag names separated by a space and hit enter

At least two molecules of the RNA helicase Has1 are simultaneously present in pre-ribosomes during ribosome biogenesis.

Abstract

The RNA helicase Has1 is involved in the biogenesis of both small and large ribosomal subunits. How it performs these separate roles is not fully understood. Here we provide evidence that at least two molecules of Has1 are temporarily present at the same time in 90S pre-ribosomes. We identified multiple Has1 binding sites in the 18S, 5.8S and 25S rRNAs. We show that while the Has1 catalytic activity is not required for binding to 5.8S/25S region in pre-rRNA, it is essential for binding to 18S sites. After the cleavage of pre-rRNA at the A2 site, Has1 remains associated not only with pre-60S but, unexpectedly, also with pre-40S ribosomes. The recruitment to 90S/pre-40S and pre-60S ribosomes is mutually independent. Our data provides insight into how Has1 performs its separate functions in the synthesis of both ribosomal subunits.

Authors+Show Affiliations

Biochemistry Center, University of Heidelberg, Im Neuenheimer Feld 328, 69120 Heidelberg, Germany. Inserm UMR1131, Institute Universitaire d'Hématologie, Hôpital St. Louis, F-75010 Paris, France.Biochemistry Center, University of Heidelberg, Im Neuenheimer Feld 328, 69120 Heidelberg, Germany.Biochemistry Center, University of Heidelberg, Im Neuenheimer Feld 328, 69120 Heidelberg, Germany.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31511893

Citation

Gnanasundram, Sivakumar Vadivel, et al. "At Least Two Molecules of the RNA Helicase Has1 Are Simultaneously Present in Pre-ribosomes During Ribosome Biogenesis." Nucleic Acids Research, 2019.
Gnanasundram SV, Kos-Braun IC, Koš M. At least two molecules of the RNA helicase Has1 are simultaneously present in pre-ribosomes during ribosome biogenesis. Nucleic Acids Res. 2019.
Gnanasundram, S. V., Kos-Braun, I. C., & Koš, M. (2019). At least two molecules of the RNA helicase Has1 are simultaneously present in pre-ribosomes during ribosome biogenesis. Nucleic Acids Research, doi:10.1093/nar/gkz767.
Gnanasundram SV, Kos-Braun IC, Koš M. At Least Two Molecules of the RNA Helicase Has1 Are Simultaneously Present in Pre-ribosomes During Ribosome Biogenesis. Nucleic Acids Res. 2019 Sep 12; PubMed PMID: 31511893.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - At least two molecules of the RNA helicase Has1 are simultaneously present in pre-ribosomes during ribosome biogenesis. AU - Gnanasundram,Sivakumar Vadivel, AU - Kos-Braun,Isabelle C, AU - Koš,Martin, Y1 - 2019/09/12/ PY - 2019/08/22/accepted PY - 2019/08/20/revised PY - 2019/04/05/received PY - 2019/9/13/entrez JF - Nucleic acids research JO - Nucleic Acids Res. N2 - The RNA helicase Has1 is involved in the biogenesis of both small and large ribosomal subunits. How it performs these separate roles is not fully understood. Here we provide evidence that at least two molecules of Has1 are temporarily present at the same time in 90S pre-ribosomes. We identified multiple Has1 binding sites in the 18S, 5.8S and 25S rRNAs. We show that while the Has1 catalytic activity is not required for binding to 5.8S/25S region in pre-rRNA, it is essential for binding to 18S sites. After the cleavage of pre-rRNA at the A2 site, Has1 remains associated not only with pre-60S but, unexpectedly, also with pre-40S ribosomes. The recruitment to 90S/pre-40S and pre-60S ribosomes is mutually independent. Our data provides insight into how Has1 performs its separate functions in the synthesis of both ribosomal subunits. SN - 1362-4962 UR - https://www.unboundmedicine.com/medline/citation/31511893/At_least_two_molecules_of_the_RNA_helicase_Has1_are_simultaneously_present_in_pre-ribosomes_during_ribosome_biogenesis L2 - https://academic.oup.com/nar/article-lookup/doi/10.1093/nar/gkz767 DB - PRIME DP - Unbound Medicine ER -