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[Effect of icariin/attapulgite/collagen type Ⅰ/polycaprolactone composite scaffold in repair of rabbit tibia defect].
Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2019 Sep 15; 33(9):1181-1189.ZX

Abstract

Objective

To investigate the effect of icarin/attapulgite/collagen type Ⅰ/polycaprolactone (ICA/ATP/Col Ⅰ/PCL) composite scaffold in repair of rabbit tibia defect.

Methods

The ICA/20%ATP/Col Ⅰ/PCL (scaffold 1), ICA/30%ATP/Col Ⅰ/PCL (scaffold 2), 20%ATP/Col Ⅰ/PCL (scaffold 3), and 30%ATP/Col Ⅰ/PCL (scaffold 4) composite scaffolds were constructed by solution casting-particle filtration method. The structure characteristics of the scaffold 2 before and after cross-linking were observed by scanning electron microscopy, and the surface contact angles of the scaffold 2 and the scaffold 4 were used to evaluate the water absorption performance of the material. The in vitro degradation test was used to evaluate the sustained-release effect of the scaffold 2. Thirty male Japanese white rabbits, weighing (2.0±0.1) kg, were randomly divided into groups A, B, C, D, and E, 6 in each group. After making a 1 cm- diameter bilateral tibial defects model, group A was the defect control group without any material implanted. Groups B, C, D, and E were implanted with scaffolds 3, 4, 1, and 2 at the defect sites, respectively. At 4, 8, and 12 weeks after operation, the repairing effects of 4 scaffolds were observed by gross observation, histological observation of HE and Masson staining, and immunohistochemical staining of osteogenic specific transcription factor (runt-related transcription factor 2, RUNX2), osteogenic related transcription factor [Osterix (OSX), Col Ⅰ, osteopontin (OPN)].

Results

Scanning electron microscopy observation showed that the scaffolds were all porous. The structure of the material was loose before and after cross-linking. The surface contact angle showed that the scaffold was hydrophobic, and the scaffold 2 was more hydrophobic than scaffold 4. The sustained-release effect in vitro showed that the drug could be released in a micro and long-term manner. In the animal implantation experiment, the gross observation showed that the defects were significantly smaller in groups D and E than in groups A, B, and C at 4 and 12 weeks after operation. HE and Masson staining showed that the defect of group A was full of connective tissue at 4 weeks after operation, a large number of fibers were seen in groups B and C, and the new bone formation was observed in groups D and E. The increase of new bone was observed in each group at 8 weeks after operation. The defect of group A was still dominated by connective tissue at 12 weeks after operation, and a small amount of new bone tissue was observed in groups B and C, and a large number of new bone tissue was observed in groups D and E, especially in group E, and most of the materials degraded. Immunohistochemical staining showed that the expressions of RUNX2 and OSX in the new tissues of groups D and E were significantly higher than those of the other groups at 4 weeks after operation. The expression of RUNX2 decreased at 8 and 12 weeks after operation. After 8 weeks and 12 weeks, the expressions of Col Ⅰand OPN increased than in 4 weeks. And the expressions of Col Ⅰ and OPN in the new tissues of groups D and E were significantly more than those of the other groups.

Conclusion

ICA/ATP/Col I/PCL composite scaffolds have good porosity and biocompatibility, can promote bone formation, and have good bone regeneration and repair effect.

Authors+Show Affiliations

School of Basic Medical Sciences, Gansu University of Traditional Chinese Medicine, Lanzhou Gansu, 730000, P.R.China;Department of Orthopaedic Laboratory, Changzhou Second People's Hospital, Changzhou Jiangsu, 213000, P.R.China.Department of Orthopaedic Laboratory, Changzhou Second People's Hospital, Changzhou Jiangsu, 213000, P.R.China.Department of Orthopaedic Laboratory, Changzhou Second People's Hospital, Changzhou Jiangsu, 213000, P.R.China.Department of Orthopaedic Laboratory, Changzhou Second People's Hospital, Changzhou Jiangsu, 213000, P.R.China.Department of Orthopaedic Laboratory, Changzhou Second People's Hospital, Changzhou Jiangsu, 213000, P.R.China.School of Basic Medical Sciences, Gansu University of Traditional Chinese Medicine, Lanzhou Gansu, 730000, P.R.China;Department of Orthopaedic Laboratory, Changzhou Second People's Hospital, Changzhou Jiangsu, 213000, P.R.China.zhao761032@163.com.

Pub Type(s)

Journal Article

Language

chi

PubMed ID

31512463

Citation

Ning, Yu, et al. "[Effect of Icariin/attapulgite/collagen Type Ⅰ/polycaprolactone Composite Scaffold in Repair of Rabbit Tibia Defect]." Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi = Zhongguo Xiufu Chongjian Waike Zazhi = Chinese Journal of Reparative and Reconstructive Surgery, vol. 33, no. 9, 2019, pp. 1181-1189.
Ning Y, Qin W, Ren Y, et al. [Effect of icariin/attapulgite/collagen type Ⅰ/polycaprolactone composite scaffold in repair of rabbit tibia defect]. Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2019;33(9):1181-1189.
Ning, Y., Qin, W., Ren, Y., Li, C., Chen, W., & Zhao, H. (2019). [Effect of icariin/attapulgite/collagen type Ⅰ/polycaprolactone composite scaffold in repair of rabbit tibia defect]. Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi = Zhongguo Xiufu Chongjian Waike Zazhi = Chinese Journal of Reparative and Reconstructive Surgery, 33(9), 1181-1189. https://doi.org/10.7507/1002-1892.201902044
Ning Y, et al. [Effect of Icariin/attapulgite/collagen Type Ⅰ/polycaprolactone Composite Scaffold in Repair of Rabbit Tibia Defect]. Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2019 Sep 15;33(9):1181-1189. PubMed PMID: 31512463.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Effect of icariin/attapulgite/collagen type Ⅰ/polycaprolactone composite scaffold in repair of rabbit tibia defect]. AU - Ning,Yu, AU - Qin,Wen, AU - Ren,Yahui, AU - Li,Chenkai, AU - Chen,Wenyang, AU - Zhao,Hongbin, PY - 2019/9/13/entrez PY - 2019/9/13/pubmed PY - 2019/11/7/medline KW - Icariin KW - attapulgite KW - collagen typeⅠ KW - composite scaffold KW - polycaprolactone KW - tissue engineered bone SP - 1181 EP - 1189 JF - Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = Chinese journal of reparative and reconstructive surgery JO - Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi VL - 33 IS - 9 N2 - Objective: To investigate the effect of icarin/attapulgite/collagen type Ⅰ/polycaprolactone (ICA/ATP/Col Ⅰ/PCL) composite scaffold in repair of rabbit tibia defect. Methods: The ICA/20%ATP/Col Ⅰ/PCL (scaffold 1), ICA/30%ATP/Col Ⅰ/PCL (scaffold 2), 20%ATP/Col Ⅰ/PCL (scaffold 3), and 30%ATP/Col Ⅰ/PCL (scaffold 4) composite scaffolds were constructed by solution casting-particle filtration method. The structure characteristics of the scaffold 2 before and after cross-linking were observed by scanning electron microscopy, and the surface contact angles of the scaffold 2 and the scaffold 4 were used to evaluate the water absorption performance of the material. The in vitro degradation test was used to evaluate the sustained-release effect of the scaffold 2. Thirty male Japanese white rabbits, weighing (2.0±0.1) kg, were randomly divided into groups A, B, C, D, and E, 6 in each group. After making a 1 cm- diameter bilateral tibial defects model, group A was the defect control group without any material implanted. Groups B, C, D, and E were implanted with scaffolds 3, 4, 1, and 2 at the defect sites, respectively. At 4, 8, and 12 weeks after operation, the repairing effects of 4 scaffolds were observed by gross observation, histological observation of HE and Masson staining, and immunohistochemical staining of osteogenic specific transcription factor (runt-related transcription factor 2, RUNX2), osteogenic related transcription factor [Osterix (OSX), Col Ⅰ, osteopontin (OPN)]. Results: Scanning electron microscopy observation showed that the scaffolds were all porous. The structure of the material was loose before and after cross-linking. The surface contact angle showed that the scaffold was hydrophobic, and the scaffold 2 was more hydrophobic than scaffold 4. The sustained-release effect in vitro showed that the drug could be released in a micro and long-term manner. In the animal implantation experiment, the gross observation showed that the defects were significantly smaller in groups D and E than in groups A, B, and C at 4 and 12 weeks after operation. HE and Masson staining showed that the defect of group A was full of connective tissue at 4 weeks after operation, a large number of fibers were seen in groups B and C, and the new bone formation was observed in groups D and E. The increase of new bone was observed in each group at 8 weeks after operation. The defect of group A was still dominated by connective tissue at 12 weeks after operation, and a small amount of new bone tissue was observed in groups B and C, and a large number of new bone tissue was observed in groups D and E, especially in group E, and most of the materials degraded. Immunohistochemical staining showed that the expressions of RUNX2 and OSX in the new tissues of groups D and E were significantly higher than those of the other groups at 4 weeks after operation. The expression of RUNX2 decreased at 8 and 12 weeks after operation. After 8 weeks and 12 weeks, the expressions of Col Ⅰand OPN increased than in 4 weeks. And the expressions of Col Ⅰ and OPN in the new tissues of groups D and E were significantly more than those of the other groups. Conclusion: ICA/ATP/Col I/PCL composite scaffolds have good porosity and biocompatibility, can promote bone formation, and have good bone regeneration and repair effect. SN - 1002-1892 UR - https://www.unboundmedicine.com/medline/citation/31512463/[Effect_of_icariin/attapulgite/collagen_type_Ⅰ/polycaprolactone_composite_scaffold_in_repair_of_rabbit_tibia_defect] DB - PRIME DP - Unbound Medicine ER -