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Effect of Mobile Phase pH on the Electrospray Ionization Efficiency and Qualitative Analysis of Pharmaceuticals in ESI + LC-MS/MS.
J Chromatogr Sci. 2019 Oct 17; 57(9):847-854.JC

Abstract

The effect of mobile phase pH on positive ionization process and retention time of nine pharmaceuticals on ultra-performance liquid chromatography-electrospray-tandem mass spectrometry (LC-MS/MS) was discussed. The effective use of high and low mobile phase pH in LC-MS/MS qualitative analyses method was also evaluated by comparing the instrument detection limit, quantification limit, precision, linearity and signal to noise (S/N) under low and high mobile phase pH. In this work, six mobile phase pH that ranged between pH 2 and pH 10 were used to evaluate the effect of the mobile phase pH changes on the ionization process in electrospray ionization. Results revealed that high mobile phase pH ionized more pharmaceuticals molecules and gave a higher signal than low mobile phase pH in positive ionization mode. The results proved that ammonium ion was better as a proton donor in high pH mobile phase than the hydronium ion in acidic mobile phase. The results revealed that the qualitative LC-MS/MS analyses method by using high mobile phase pH has better performance for most analytes in terms of sensitivity, precision, linearity and S/N than the low mobile phase pH.

Authors+Show Affiliations

Department of Chemistry, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia.Faculty of Applied Sciences, Universiti Teknologi MARA, 40450 Shah Alam, Selangor, Malaysia.Department of Chemistry, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31512731

Citation

Kafeenah, Husam I S., et al. "Effect of Mobile Phase pH On the Electrospray Ionization Efficiency and Qualitative Analysis of Pharmaceuticals in ESI + LC-MS/MS." Journal of Chromatographic Science, vol. 57, no. 9, 2019, pp. 847-854.
Kafeenah HIS, Osman R, Bakar NKA. Effect of Mobile Phase pH on the Electrospray Ionization Efficiency and Qualitative Analysis of Pharmaceuticals in ESI + LC-MS/MS. J Chromatogr Sci. 2019;57(9):847-854.
Kafeenah, H. I. S., Osman, R., & Bakar, N. K. A. (2019). Effect of Mobile Phase pH on the Electrospray Ionization Efficiency and Qualitative Analysis of Pharmaceuticals in ESI + LC-MS/MS. Journal of Chromatographic Science, 57(9), 847-854. https://doi.org/10.1093/chromsci/bmz061
Kafeenah HIS, Osman R, Bakar NKA. Effect of Mobile Phase pH On the Electrospray Ionization Efficiency and Qualitative Analysis of Pharmaceuticals in ESI + LC-MS/MS. J Chromatogr Sci. 2019 Oct 17;57(9):847-854. PubMed PMID: 31512731.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effect of Mobile Phase pH on the Electrospray Ionization Efficiency and Qualitative Analysis of Pharmaceuticals in ESI + LC-MS/MS. AU - Kafeenah,Husam I S, AU - Osman,Rozita, AU - Bakar,N K A, PY - 2018/12/18/received PY - 2019/05/31/revised PY - 2019/07/01/accepted PY - 2019/9/13/pubmed PY - 2020/1/25/medline PY - 2019/9/13/entrez SP - 847 EP - 854 JF - Journal of chromatographic science JO - J Chromatogr Sci VL - 57 IS - 9 N2 - The effect of mobile phase pH on positive ionization process and retention time of nine pharmaceuticals on ultra-performance liquid chromatography-electrospray-tandem mass spectrometry (LC-MS/MS) was discussed. The effective use of high and low mobile phase pH in LC-MS/MS qualitative analyses method was also evaluated by comparing the instrument detection limit, quantification limit, precision, linearity and signal to noise (S/N) under low and high mobile phase pH. In this work, six mobile phase pH that ranged between pH 2 and pH 10 were used to evaluate the effect of the mobile phase pH changes on the ionization process in electrospray ionization. Results revealed that high mobile phase pH ionized more pharmaceuticals molecules and gave a higher signal than low mobile phase pH in positive ionization mode. The results proved that ammonium ion was better as a proton donor in high pH mobile phase than the hydronium ion in acidic mobile phase. The results revealed that the qualitative LC-MS/MS analyses method by using high mobile phase pH has better performance for most analytes in terms of sensitivity, precision, linearity and S/N than the low mobile phase pH. SN - 1945-239X UR - https://www.unboundmedicine.com/medline/citation/31512731/Effect_of_Mobile_Phase_pH_on_the_Electrospray_Ionization_Efficiency_and_Qualitative_Analysis_of_Pharmaceuticals_in_ESI_+_LC-MS/MS L2 - https://academic.oup.com/chromsci/article-lookup/doi/10.1093/chromsci/bmz061 DB - PRIME DP - Unbound Medicine ER -