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Anti-Haemophilus Activity of Selected Essential Oils Detected by TLC-Direct Bioautography and Biofilm Inhibition.
Molecules 2019; 24(18)M

Abstract

Essential oils (EOs) are becoming increasingly popular in medical applications because of their antimicrobial effect. Direct bioautography (DB) combined with thin layer chromatography (TLC) is a screening method for the detection of antimicrobial compounds in plant extracts, for example, in EOs. Due to their lipophilic character, the common microbiological assays (etc. disk diffusion) could not provide reliable results. The aim of this study was the evaluation of antibacterial and anti-biofilm properties of the EO of cinnamon bark, clove, peppermint, thyme, and their main components against Haemophilus influenzae and H. parainfluenzae. Oil in water (O/W) type Pickering nano-emulsions stabilized with silica nanoparticles from each oil were prepared to increase their water-solubility. Samples with Tween80 surfactant and absolute ethanol were also used. Results showed that H. influenzae was more sensitive to the EOs than H. parainfluenzae (except for cinnamon bark oil). In thin layer chromatography-direct bioautography (TLC-DB) the ethanolic solutions of thyme oil presented the best activity against H. influenzae, while cinnamon oil was the most active against H. parainfluenzae. Pickering nano-emulsion of cinnamon oil inhibited the biofilm formation of H. parainfluenzae (76.35%) more efficiently than samples with Tween80 surfactant or absolute ethanol. In conclusion, Pickering nano-emulsion of EOs could inhibit the biofilm production effectively.

Authors+Show Affiliations

Department of Pharmacognosy, Faculty of Pharmacy, University of Pécs, H-7624 Pécs, Hungary. balazsviktorialilla@gmail.com.Institute of Pharmaceutical Technology and Biopharmacy, Faculty of Pharmacy, University of Pécs, H-7624 Pécs, Hungary. bai0311@gmail.com.Department of Microbiology, Faculty of Science and Informatics, University of Szeged, H-6726 Szeged, Hungary. kerekeserika88@gmail.com.Department of Pharmacognosy, Faculty of Pharmacy, University of Pécs, H-7624 Pécs, Hungary.Department of Medical Microbiology and Immunology, Medical School, University of Pécs, H-7624 Pécs, Hungary. kocsis.bela@pte.hu.Department of Medical Microbiology and Immunology, Medical School, University of Pécs, H-7624 Pécs, Hungary. adorjanvarga@ymail.com.Institute of Pharmacognosy, Faculty of Pharmacy, Semmelweis University, H-1085 Budapest, Hungary. aboszormenyi@gmail.com.Department of Genetics and Molecular Biology, Institute of Biology, Faculty of Sciences, University of Pécs, H-7624 Pécs, Hungary. davenagy9@gmail.com.Department of Food Engineering, Faculty of Engineering, University of Szeged, H-6724 Szeged, Hungary. krisch@mk.u-szeged.hu.Institute of Pharmaceutical Technology and Biopharmacy, Faculty of Pharmacy, University of Pécs, H-7624 Pécs, Hungary. szealex@gamma.ttk.pte.hu.Department of Pharmacognosy, Faculty of Pharmacy, University of Pécs, H-7624 Pécs, Hungary. horvath.gyorgyi@gytk.pte.hu.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31514307

Citation

Balázs, Viktória Lilla, et al. "Anti-Haemophilus Activity of Selected Essential Oils Detected By TLC-Direct Bioautography and Biofilm Inhibition." Molecules (Basel, Switzerland), vol. 24, no. 18, 2019.
Balázs VL, Horváth B, Kerekes E, et al. Anti-Haemophilus Activity of Selected Essential Oils Detected by TLC-Direct Bioautography and Biofilm Inhibition. Molecules. 2019;24(18).
Balázs, V. L., Horváth, B., Kerekes, E., Ács, K., Kocsis, B., Varga, A., ... Horváth, G. (2019). Anti-Haemophilus Activity of Selected Essential Oils Detected by TLC-Direct Bioautography and Biofilm Inhibition. Molecules (Basel, Switzerland), 24(18), doi:10.3390/molecules24183301.
Balázs VL, et al. Anti-Haemophilus Activity of Selected Essential Oils Detected By TLC-Direct Bioautography and Biofilm Inhibition. Molecules. 2019 Sep 11;24(18) PubMed PMID: 31514307.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Anti-Haemophilus Activity of Selected Essential Oils Detected by TLC-Direct Bioautography and Biofilm Inhibition. AU - Balázs,Viktória Lilla, AU - Horváth,Barbara, AU - Kerekes,Erika, AU - Ács,Kamilla, AU - Kocsis,Béla, AU - Varga,Adorján, AU - Böszörményi,Andrea, AU - Nagy,Dávid U, AU - Krisch,Judit, AU - Széchenyi,Aleksandar, AU - Horváth,Györgyi, Y1 - 2019/09/11/ PY - 2019/08/07/received PY - 2019/09/05/revised PY - 2019/09/10/accepted PY - 2019/9/14/entrez KW - Haemophilus influenzae KW - Haemophilus parainfluenzae KW - Pickering nano-emulsion KW - anti-biofilm activity KW - cinnamon bark KW - clove KW - essential oil KW - peppermint KW - thyme JF - Molecules (Basel, Switzerland) JO - Molecules VL - 24 IS - 18 N2 - Essential oils (EOs) are becoming increasingly popular in medical applications because of their antimicrobial effect. Direct bioautography (DB) combined with thin layer chromatography (TLC) is a screening method for the detection of antimicrobial compounds in plant extracts, for example, in EOs. Due to their lipophilic character, the common microbiological assays (etc. disk diffusion) could not provide reliable results. The aim of this study was the evaluation of antibacterial and anti-biofilm properties of the EO of cinnamon bark, clove, peppermint, thyme, and their main components against Haemophilus influenzae and H. parainfluenzae. Oil in water (O/W) type Pickering nano-emulsions stabilized with silica nanoparticles from each oil were prepared to increase their water-solubility. Samples with Tween80 surfactant and absolute ethanol were also used. Results showed that H. influenzae was more sensitive to the EOs than H. parainfluenzae (except for cinnamon bark oil). In thin layer chromatography-direct bioautography (TLC-DB) the ethanolic solutions of thyme oil presented the best activity against H. influenzae, while cinnamon oil was the most active against H. parainfluenzae. Pickering nano-emulsion of cinnamon oil inhibited the biofilm formation of H. parainfluenzae (76.35%) more efficiently than samples with Tween80 surfactant or absolute ethanol. In conclusion, Pickering nano-emulsion of EOs could inhibit the biofilm production effectively. SN - 1420-3049 UR - https://www.unboundmedicine.com/medline/citation/31514307/Anti-Haemophilus_Activity_of_Selected_Essential_Oils_Detected_by_TLC-Direct_Bioautography_and_Biofilm_Inhibition L2 - http://www.mdpi.com/resolver?pii=molecules24183301 DB - PRIME DP - Unbound Medicine ER -