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Mechanism of high folate accumulation in a sake yeast other than Kyokai yeasts.

Abstract

Folates are important vitamins in human nutrition. Pressed sake cake, a brewing by-product of sake, is a rich dietary source of folates derived from sake yeast (Saccharomyces cerevisiae). The National Research Institute of Brewing investigated 106 samples of pressed sake cake and revealed that three samples containing large amounts of folates were produced by Km67 yeast derived from the house sake yeast strain of Kiku-Masamune sake brewery. In this study, we performed sake brewing tests using Km67 and Kyokai no. 7 group strains and confirmed that Km67 yeast contributed to the production of pressed sake cake containing large amounts of folates. To elucidate the mechanisms of high folate accumulation in Km67, we performed whole-genome sequence analysis in Km67 and then screened 10 folate-metabolizing genes showing different sequences in Km67 and K7 strains. By folate analysis of each gene-disrupted strain derived from strain BY4743, we also selected four genes having significant effects on folate content in yeast from 10 candidate genes. Folate analysis of gene-disrupted yeast strains complemented with either Km67-type genes or K7-type genes revealed that the Km67-type HMT1 gene was related to high folate accumulation not only in laboratory yeast but also in sake yeast. In this gene, Leu63Phe was present in the methyltransferase motif I of Hmt1p, which was essential for the methyltransferase activity of Hmt1p. Our results and previous reports suggested that the methyltransferase activity of Km67-Hmt1p was higher than that of K7-Hmt1p, leading to enhanced production and high accumulation of folates in Km67 yeast.

Authors+Show Affiliations

General Research Laboratory, Kiku-Masamune Sake Brewing Co. Ltd., 1-8-6 Uozaki-nishimachi, Higashinada-ku, Kobe, Hyogo 658-0026, Japan. Electronic address: y-shibata@kikumasamune.co.jp.General Research Laboratory, Kiku-Masamune Sake Brewing Co. Ltd., 1-8-6 Uozaki-nishimachi, Higashinada-ku, Kobe, Hyogo 658-0026, Japan.National Research Institute of Brewing, 3-7-1 Kagamiyama, Higashihiroshima, Hiroshima 739-0046, Japan.National Research Institute of Brewing, 3-7-1 Kagamiyama, Higashihiroshima, Hiroshima 739-0046, Japan.National Research Institute of Brewing, 3-7-1 Kagamiyama, Higashihiroshima, Hiroshima 739-0046, Japan.National Research Institute of Brewing, 3-7-1 Kagamiyama, Higashihiroshima, Hiroshima 739-0046, Japan.General Research Laboratory, Kiku-Masamune Sake Brewing Co. Ltd., 1-8-6 Uozaki-nishimachi, Higashinada-ku, Kobe, Hyogo 658-0026, Japan.General Research Laboratory, Kiku-Masamune Sake Brewing Co. Ltd., 1-8-6 Uozaki-nishimachi, Higashinada-ku, Kobe, Hyogo 658-0026, Japan.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31515157

Citation

Shibata, Yusuke, et al. "Mechanism of High Folate Accumulation in a Sake Yeast Other Than Kyokai Yeasts." Journal of Bioscience and Bioengineering, 2019.
Shibata Y, Yamada T, Morimoto T, et al. Mechanism of high folate accumulation in a sake yeast other than Kyokai yeasts. J Biosci Bioeng. 2019.
Shibata, Y., Yamada, T., Morimoto, T., Fujii, T., Akao, T., Goshima, T., ... Tanaka, N. (2019). Mechanism of high folate accumulation in a sake yeast other than Kyokai yeasts. Journal of Bioscience and Bioengineering, doi:10.1016/j.jbiosc.2019.07.008.
Shibata Y, et al. Mechanism of High Folate Accumulation in a Sake Yeast Other Than Kyokai Yeasts. J Biosci Bioeng. 2019 Sep 9; PubMed PMID: 31515157.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Mechanism of high folate accumulation in a sake yeast other than Kyokai yeasts. AU - Shibata,Yusuke, AU - Yamada,Tasuku, AU - Morimoto,Tomoko, AU - Fujii,Tsutomu, AU - Akao,Takeshi, AU - Goshima,Tetsuya, AU - Takahashi,Toshinari, AU - Tanaka,Nobuchika, Y1 - 2019/09/09/ PY - 2019/05/24/received PY - 2019/07/18/revised PY - 2019/07/30/accepted PY - 2019/9/14/entrez KW - Folate KW - HMT1 KW - Pressed sake cake KW - Saccharomyces cerevisiae KW - Sake yeast JF - Journal of bioscience and bioengineering JO - J. Biosci. Bioeng. N2 - Folates are important vitamins in human nutrition. Pressed sake cake, a brewing by-product of sake, is a rich dietary source of folates derived from sake yeast (Saccharomyces cerevisiae). The National Research Institute of Brewing investigated 106 samples of pressed sake cake and revealed that three samples containing large amounts of folates were produced by Km67 yeast derived from the house sake yeast strain of Kiku-Masamune sake brewery. In this study, we performed sake brewing tests using Km67 and Kyokai no. 7 group strains and confirmed that Km67 yeast contributed to the production of pressed sake cake containing large amounts of folates. To elucidate the mechanisms of high folate accumulation in Km67, we performed whole-genome sequence analysis in Km67 and then screened 10 folate-metabolizing genes showing different sequences in Km67 and K7 strains. By folate analysis of each gene-disrupted strain derived from strain BY4743, we also selected four genes having significant effects on folate content in yeast from 10 candidate genes. Folate analysis of gene-disrupted yeast strains complemented with either Km67-type genes or K7-type genes revealed that the Km67-type HMT1 gene was related to high folate accumulation not only in laboratory yeast but also in sake yeast. In this gene, Leu63Phe was present in the methyltransferase motif I of Hmt1p, which was essential for the methyltransferase activity of Hmt1p. Our results and previous reports suggested that the methyltransferase activity of Km67-Hmt1p was higher than that of K7-Hmt1p, leading to enhanced production and high accumulation of folates in Km67 yeast. SN - 1347-4421 UR - https://www.unboundmedicine.com/medline/citation/31515157/Mechanism_of_high_folate_accumulation_in_a_sake_yeast_other_than_Kyokai_yeasts L2 - https://linkinghub.elsevier.com/retrieve/pii/S1389-1723(19)30477-3 DB - PRIME DP - Unbound Medicine ER -