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Genetic dissection of a major QTL for kernel weight spanning the Rht-B1 locus in bread wheat.
Theor Appl Genet. 2019 Nov; 132(11):3191-3200.TA

Abstract

KEY MESSAGE

Genetic dissection uncovered a major QTL QTKW.caas-4BS corresponding with a 483 kb deletion that included genes ZnF, EamA and Rht-B1. This deletion was associated with increased grain weight and semi-dwarf phenotype. Previous studies identified quantitative trait loci (QTL) for thousand kernel weight (TKW) in the region spanning the Rht-B1 locus in wheat (Triticum aestivum L.). We recently mapped a major QTL QTKW.caas-4BS for TKW spanning the Rht-B1 locus in a recombinant inbred line (RIL) population derived from Doumai/Shi 4185 using the wheat 90K array. The allele from Doumai at QTKW.caas-4BS significantly increased TKW and kernel number per spike, and conferred semi-dwarf trait, which was beneficial to improve grain yield without a penalty to lodging. To further dissect QTKW.caas-4BS, we firstly re-investigated the genotypes and phenotypes of the RILs and confirmed the QTL using cleaved amplified polymorphic sequence (CAPS) markers developed from flanking SNP markers IWA102 and IWB54814. The target sequences of the CAPS markers were used as queries to BLAST the wheat reference genome RefSeq v1.0 and hit an approximate 10.4 Mb genomic region. Based on genomic mining and SNP loci from the wheat 660K SNP array in the above genomic region, we developed eight new markers and narrowed QTKW.caas-4BS to a genetic interval of 1.5 cM. A 483 kb deletion in Doumai corresponded with QTKW.caas-4BS genetically, including three genes ZnF, EamA and Rht-B1. The other 15 genes with either differential expressions and/or sequence variations between parents were also potential candidate genes for QTKW.caas-4BS. The findings not only provide a toolkit for marker-assisted selection of QTKW.caas-4BS but also defined candidate genes for further functional analysis.

Authors+Show Affiliations

Institute of Crop Science, National Wheat Improvement Center, Chinese Academy of Agricultural Sciences (CAAS), 12 Zhongguancun South Street, Beijing, 100081, China. Department of Plant Genetics & Breeding/State Key Laboratory for Agrobiotechnology, China Agricultural University, 2 Yuanmingyuan West Road, Beijing, 100094, China.Institute of Crop Science, National Wheat Improvement Center, Chinese Academy of Agricultural Sciences (CAAS), 12 Zhongguancun South Street, Beijing, 100081, China.Institute of Crop Science, National Wheat Improvement Center, Chinese Academy of Agricultural Sciences (CAAS), 12 Zhongguancun South Street, Beijing, 100081, China.Institute of Crop Science, National Wheat Improvement Center, Chinese Academy of Agricultural Sciences (CAAS), 12 Zhongguancun South Street, Beijing, 100081, China.Institute of Crop Science, National Wheat Improvement Center, Chinese Academy of Agricultural Sciences (CAAS), 12 Zhongguancun South Street, Beijing, 100081, China.Institute of Crop Science, National Wheat Improvement Center, Chinese Academy of Agricultural Sciences (CAAS), 12 Zhongguancun South Street, Beijing, 100081, China.Institute of Crop Science, National Wheat Improvement Center, Chinese Academy of Agricultural Sciences (CAAS), 12 Zhongguancun South Street, Beijing, 100081, China.Department of Plant Genetics & Breeding/State Key Laboratory for Agrobiotechnology, China Agricultural University, 2 Yuanmingyuan West Road, Beijing, 100094, China.Institute of Crop Science, National Wheat Improvement Center, Chinese Academy of Agricultural Sciences (CAAS), 12 Zhongguancun South Street, Beijing, 100081, China. zhhecaas@163.com.Institute of Crop Science, National Wheat Improvement Center, Chinese Academy of Agricultural Sciences (CAAS), 12 Zhongguancun South Street, Beijing, 100081, China. shcao8@163.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31515582

Citation

Xu, Dengan, et al. "Genetic Dissection of a Major QTL for Kernel Weight Spanning the Rht-B1 Locus in Bread Wheat." TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, vol. 132, no. 11, 2019, pp. 3191-3200.
Xu D, Wen W, Fu L, et al. Genetic dissection of a major QTL for kernel weight spanning the Rht-B1 locus in bread wheat. Theor Appl Genet. 2019;132(11):3191-3200.
Xu, D., Wen, W., Fu, L., Li, F., Li, J., Xie, L., Xia, X., Ni, Z., He, Z., & Cao, S. (2019). Genetic dissection of a major QTL for kernel weight spanning the Rht-B1 locus in bread wheat. TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, 132(11), 3191-3200. https://doi.org/10.1007/s00122-019-03418-w
Xu D, et al. Genetic Dissection of a Major QTL for Kernel Weight Spanning the Rht-B1 Locus in Bread Wheat. Theor Appl Genet. 2019;132(11):3191-3200. PubMed PMID: 31515582.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Genetic dissection of a major QTL for kernel weight spanning the Rht-B1 locus in bread wheat. AU - Xu,Dengan, AU - Wen,Weie, AU - Fu,Luping, AU - Li,Faji, AU - Li,Jihu, AU - Xie,Li, AU - Xia,Xianchun, AU - Ni,Zhongfu, AU - He,Zhonghu, AU - Cao,Shuanghe, Y1 - 2019/09/12/ PY - 2019/01/16/received PY - 2019/08/27/accepted PY - 2019/07/28/revised PY - 2019/9/14/pubmed PY - 2020/2/6/medline PY - 2019/9/14/entrez SP - 3191 EP - 3200 JF - TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik JO - Theor Appl Genet VL - 132 IS - 11 N2 - KEY MESSAGE: Genetic dissection uncovered a major QTL QTKW.caas-4BS corresponding with a 483 kb deletion that included genes ZnF, EamA and Rht-B1. This deletion was associated with increased grain weight and semi-dwarf phenotype. Previous studies identified quantitative trait loci (QTL) for thousand kernel weight (TKW) in the region spanning the Rht-B1 locus in wheat (Triticum aestivum L.). We recently mapped a major QTL QTKW.caas-4BS for TKW spanning the Rht-B1 locus in a recombinant inbred line (RIL) population derived from Doumai/Shi 4185 using the wheat 90K array. The allele from Doumai at QTKW.caas-4BS significantly increased TKW and kernel number per spike, and conferred semi-dwarf trait, which was beneficial to improve grain yield without a penalty to lodging. To further dissect QTKW.caas-4BS, we firstly re-investigated the genotypes and phenotypes of the RILs and confirmed the QTL using cleaved amplified polymorphic sequence (CAPS) markers developed from flanking SNP markers IWA102 and IWB54814. The target sequences of the CAPS markers were used as queries to BLAST the wheat reference genome RefSeq v1.0 and hit an approximate 10.4 Mb genomic region. Based on genomic mining and SNP loci from the wheat 660K SNP array in the above genomic region, we developed eight new markers and narrowed QTKW.caas-4BS to a genetic interval of 1.5 cM. A 483 kb deletion in Doumai corresponded with QTKW.caas-4BS genetically, including three genes ZnF, EamA and Rht-B1. The other 15 genes with either differential expressions and/or sequence variations between parents were also potential candidate genes for QTKW.caas-4BS. The findings not only provide a toolkit for marker-assisted selection of QTKW.caas-4BS but also defined candidate genes for further functional analysis. SN - 1432-2242 UR - https://www.unboundmedicine.com/medline/citation/31515582/Genetic_dissection_of_a_major_QTL_for_kernel_weight_spanning_the_Rht_B1_locus_in_bread_wheat_ L2 - https://dx.doi.org/10.1007/s00122-019-03418-w DB - PRIME DP - Unbound Medicine ER -