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Exploratory analysis of covariation of microbiota-derived vitamin K and cognition in older adults.

Abstract

BACKGROUND

Vitamin K has multiple important physiological roles, including blood coagulation and beneficial effects on myelin integrity in the brain. Some intestinal microbes possess the genes to produce vitamin K in the form of menaquinone (MK). MK appears in higher concentration in tissues, such as the brain, particularly MK4, than the dietary form of phylloquinone (PK). Lower PK concentrations have been reported in patients with Alzheimer disease while higher serum PK concentrations have been positively associated with verbal episodic memory. Despite knowledge of the importance of vitamin K for various health parameters, few studies have measured MK concentration and biosynthesis by gut commensals.

OBJECTIVE

The aim of the current study was to investigate the relation between genes involved in gut-microbiota derived MK, concentrations of MK isoforms, and cognitive function.

METHODS

Shotgun metagenomic sequencing of the gut microbiome of 74 elderly individuals with different cognitive ability levels was performed. From this, gene counts for microbial MK biosynthesis were determined. Associations between clusters of individuals, grouped based on a similar presence and prevalence of MK biosynthesis genes, and cognitive ability were investigated. Fecal MK concentrations were quantified by HPLC to investigate correlations with subject clusters.

RESULTS

Separation of subject groups defined by banded quantification of the genetic potential of their microbiome to biosynthesize MK was associated with significant differences in cognitive ability [assessed using the Mini-Mental State Examination (MMSE)]. Three MK isoforms were found to be positively associated with MMSE, along with the identification of key components of the MK pathway that drive this association. Although the causality and direction of these associations remain unknown, these findings justify further studies.

CONCLUSIONS

This study provides evidence that although total concentrations of MK did not covary with cognition, certain MK isoforms synthesized by the gut microbiome, particularly the longer chains, are positively associated with cognition.

Authors+Show Affiliations

School of Microbiology, University College Cork, Cork, Ireland. APC Microbiome Ireland, University College Cork, Cork, Ireland.School of Microbiology, University College Cork, Cork, Ireland. APC Microbiome Ireland, University College Cork, Cork, Ireland.Département de Nutrition, Université de Montréal, Montreal, Canada.Département de Nutrition, Université de Montréal, Montreal, Canada.Jean Mayer US Department of Agriculture Human Nutrition Research Center on Aging at Tufts University, Boston, MA, USA.Jean Mayer US Department of Agriculture Human Nutrition Research Center on Aging at Tufts University, Boston, MA, USA.School of Microbiology, University College Cork, Cork, Ireland. APC Microbiome Ireland, University College Cork, Cork, Ireland.School of Microbiology, University College Cork, Cork, Ireland. APC Microbiome Ireland, University College Cork, Cork, Ireland.APC Microbiome Ireland, University College Cork, Cork, Ireland. Department of Biological Sciences, School of Natural Sciences, University of Limerick, Limerick, Ireland. Health Research Institute, University of Limerick, Limerick, Ireland.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31518386

Citation

McCann, Angela, et al. "Exploratory Analysis of Covariation of Microbiota-derived Vitamin K and Cognition in Older Adults." The American Journal of Clinical Nutrition, 2019.
McCann A, Jeffery IB, Ouliass B, et al. Exploratory analysis of covariation of microbiota-derived vitamin K and cognition in older adults. Am J Clin Nutr. 2019.
McCann, A., Jeffery, I. B., Ouliass, B., Ferland, G., Fu, X., Booth, S. L., ... O'Connor, E. M. (2019). Exploratory analysis of covariation of microbiota-derived vitamin K and cognition in older adults. The American Journal of Clinical Nutrition, doi:10.1093/ajcn/nqz220.
McCann A, et al. Exploratory Analysis of Covariation of Microbiota-derived Vitamin K and Cognition in Older Adults. Am J Clin Nutr. 2019 Sep 13; PubMed PMID: 31518386.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Exploratory analysis of covariation of microbiota-derived vitamin K and cognition in older adults. AU - McCann,Angela, AU - Jeffery,Ian B, AU - Ouliass,Bouchra, AU - Ferland,Guylaine, AU - Fu,Xueyen, AU - Booth,Sarah L, AU - Tran,Tam Tt, AU - O'Toole,Paul W, AU - O'Connor,Eibhlís M, Y1 - 2019/09/13/ PY - 2018/10/10/received PY - 2019/08/07/accepted PY - 2019/9/14/entrez PY - 2019/9/14/pubmed PY - 2019/9/14/medline KW - cognition KW - elderly KW - microbial menaquinone biosynthesis KW - shotgun metagenomic sequencing KW - vitamin K JF - The American journal of clinical nutrition JO - Am. J. Clin. Nutr. N2 - BACKGROUND: Vitamin K has multiple important physiological roles, including blood coagulation and beneficial effects on myelin integrity in the brain. Some intestinal microbes possess the genes to produce vitamin K in the form of menaquinone (MK). MK appears in higher concentration in tissues, such as the brain, particularly MK4, than the dietary form of phylloquinone (PK). Lower PK concentrations have been reported in patients with Alzheimer disease while higher serum PK concentrations have been positively associated with verbal episodic memory. Despite knowledge of the importance of vitamin K for various health parameters, few studies have measured MK concentration and biosynthesis by gut commensals. OBJECTIVE: The aim of the current study was to investigate the relation between genes involved in gut-microbiota derived MK, concentrations of MK isoforms, and cognitive function. METHODS: Shotgun metagenomic sequencing of the gut microbiome of 74 elderly individuals with different cognitive ability levels was performed. From this, gene counts for microbial MK biosynthesis were determined. Associations between clusters of individuals, grouped based on a similar presence and prevalence of MK biosynthesis genes, and cognitive ability were investigated. Fecal MK concentrations were quantified by HPLC to investigate correlations with subject clusters. RESULTS: Separation of subject groups defined by banded quantification of the genetic potential of their microbiome to biosynthesize MK was associated with significant differences in cognitive ability [assessed using the Mini-Mental State Examination (MMSE)]. Three MK isoforms were found to be positively associated with MMSE, along with the identification of key components of the MK pathway that drive this association. Although the causality and direction of these associations remain unknown, these findings justify further studies. CONCLUSIONS: This study provides evidence that although total concentrations of MK did not covary with cognition, certain MK isoforms synthesized by the gut microbiome, particularly the longer chains, are positively associated with cognition. SN - 1938-3207 UR - https://www.unboundmedicine.com/medline/citation/31518386/Exploratory_analysis_of_covariation_of_microbiota-derived_vitamin_K_and_cognition_in_older_adults L2 - https://academic.oup.com/ajcn/article-lookup/doi/10.1093/ajcn/nqz220 DB - PRIME DP - Unbound Medicine ER -