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An ultrasensitive electrochemical sensing method for detection of microcystin-LR based on infinity-shaped DNA structure using double aptamer and terminal deoxynucleotidyl transferase.
Biosens Bioelectron 2019; 144:111674BB

Abstract

This study develops a novel electrochemical sensing platform for microcystin-LR (MC-LR) detection. This aptasensor comprises the hybridization of double aptamer to its complementary strand (CS) on the surface of electrode and generation of an Infinity-shaped DNA structure in the absence of target by terminal deoxynucleotidyl transferase (TdT). The formation of Infinity-shaped construction leads to the development of an ultrasensitive aptasensor for MC-LR detection. In the presence of MC-LR, double aptamer is dissociated from its CS because of its high affinity for MC-LR and leaves the surface of electrode. Subsequently, no Infinity-shaped structure is formed following the introduction of TdT and a strong current signal is observed. The proposed method was employed for specific detection of MC-LR in the range from 60 pM to 1000 nM with a detection limit of 15 pM. The credibility of the approach was confirmed by detection of MC-LR in real samples like serum and tap water samples. This study provides a new aptasensor for detection of MC-LR as well as other toxin analysis.

Authors+Show Affiliations

Pharmaceutical Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran; Department of Medicinal Chemistry, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran.Research Institute of Sciences and New Technology, Mashhad, Iran.Pharmaceutical Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran.Pharmaceutical Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran.Pharmaceutical Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran.Academic Center for Education, Culture and Research, Research Institute for Industrial Biotechnology, Industrial Biotechnology on Microorganisms, Mashhad, Iran.Targeted Drug Delivery Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran; Department of Pharmaceutical Biotechnology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran. Electronic address: taghdisihm@mums.ac.ir.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31518788

Citation

Abnous, Khalil, et al. "An Ultrasensitive Electrochemical Sensing Method for Detection of microcystin-LR Based On Infinity-shaped DNA Structure Using Double Aptamer and Terminal Deoxynucleotidyl Transferase." Biosensors & Bioelectronics, vol. 144, 2019, p. 111674.
Abnous K, Danesh NM, Nameghi MA, et al. An ultrasensitive electrochemical sensing method for detection of microcystin-LR based on infinity-shaped DNA structure using double aptamer and terminal deoxynucleotidyl transferase. Biosens Bioelectron. 2019;144:111674.
Abnous, K., Danesh, N. M., Nameghi, M. A., Ramezani, M., Alibolandi, M., Lavaee, P., & Taghdisi, S. M. (2019). An ultrasensitive electrochemical sensing method for detection of microcystin-LR based on infinity-shaped DNA structure using double aptamer and terminal deoxynucleotidyl transferase. Biosensors & Bioelectronics, 144, p. 111674. doi:10.1016/j.bios.2019.111674.
Abnous K, et al. An Ultrasensitive Electrochemical Sensing Method for Detection of microcystin-LR Based On Infinity-shaped DNA Structure Using Double Aptamer and Terminal Deoxynucleotidyl Transferase. Biosens Bioelectron. 2019 Sep 5;144:111674. PubMed PMID: 31518788.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - An ultrasensitive electrochemical sensing method for detection of microcystin-LR based on infinity-shaped DNA structure using double aptamer and terminal deoxynucleotidyl transferase. AU - Abnous,Khalil, AU - Danesh,Noor Mohammad, AU - Nameghi,Morteza Alinezhad, AU - Ramezani,Mohammad, AU - Alibolandi,Mona, AU - Lavaee,Parirokh, AU - Taghdisi,Seyed Mohammad, Y1 - 2019/09/05/ PY - 2019/08/21/received PY - 2019/09/03/accepted PY - 2019/9/14/pubmed PY - 2019/9/14/medline PY - 2019/9/14/entrez KW - Electrochemical sensing platform KW - Infinity-shape KW - MC-LR KW - Real sample KW - TdT SP - 111674 EP - 111674 JF - Biosensors & bioelectronics JO - Biosens Bioelectron VL - 144 N2 - This study develops a novel electrochemical sensing platform for microcystin-LR (MC-LR) detection. This aptasensor comprises the hybridization of double aptamer to its complementary strand (CS) on the surface of electrode and generation of an Infinity-shaped DNA structure in the absence of target by terminal deoxynucleotidyl transferase (TdT). The formation of Infinity-shaped construction leads to the development of an ultrasensitive aptasensor for MC-LR detection. In the presence of MC-LR, double aptamer is dissociated from its CS because of its high affinity for MC-LR and leaves the surface of electrode. Subsequently, no Infinity-shaped structure is formed following the introduction of TdT and a strong current signal is observed. The proposed method was employed for specific detection of MC-LR in the range from 60 pM to 1000 nM with a detection limit of 15 pM. The credibility of the approach was confirmed by detection of MC-LR in real samples like serum and tap water samples. This study provides a new aptasensor for detection of MC-LR as well as other toxin analysis. SN - 1873-4235 UR - https://www.unboundmedicine.com/medline/citation/31518788/An_ultrasensitive_electrochemical_sensing_method_for_detection_of_microcystin-LR_based_on_infinity-shaped_DNA_structure_using_double_aptamer_and_terminal_deoxynucleotidyl_transferase L2 - https://linkinghub.elsevier.com/retrieve/pii/S0956-5663(19)30753-5 DB - PRIME DP - Unbound Medicine ER -