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Molecular detection of Treponema species organisms in foremilk and udder cleft skin of dairy cows with digital dermatitis.
Trop Anim Health Prod. 2020 Mar; 52(2):815-821.TA

Abstract

Identification of reservoirs and transmission routes of digital dermatitis (DD)-associated Treponema spp. is considered an effective means for controlling DD infection in dairy cows. The objective of this study is to identify and characterize the potential reservoir niches for DD-associated Treponema spp. from healthy udder cleft skin and foremilk in lactating dairy cows. A large dairy farm was visited weekly from March to July 2015. Clinical investigation revealed that a total of 25 lame cows had DD lesions located at the plantar aspect of the interdigital cleft. A total of 75 samples, three per cow, were collected including deep swabs from DD lesions (n = 25), non-aseptically collected foremilk samples (n = 25) and skin swabs from udder cleft (n = 25). Treponema spp. were identified using nested PCR assays and confirmed by DNA sequencing. Results revealed that Treponema phagedenis (T. phagedenis)-like was the most identified species in the foremilk 40% (10/25), in comparison with DD lesions and udder cleft skin samples with 32% (8/25) and 20% (5/25), respectively. On the other hand, Treponema pedis (T. pedis) was the most identified species in the udder cleft skin 80% (20/25), in comparison with DD lesions and foremilk samples with 68% (17/25) and 60% (15/25), respectively. None of the examined samples were identified by PCR as containing DNA from Treponema medium (T. medium) or Treponema vincentii (T. vincentii)-like. To the best of our knowledge, this is the first report for detection of T. phagedenis-like and T. pedis from healthy skin of udder cleft and foremilk samples. Detection of DD Treponema spp. from udder cleft skin and foremilk samples indicates that these sites could be potential reservoirs for spirochetes involved in DD. Udder cleft skin and foremilk may have a role in transmission routes of DD Treponema in dairy farms.

Authors+Show Affiliations

Department of Animal Medicine, Faculty of Veterinary Medicine, Zagazig University, Zagazig, Sharkia Province, 44511, Egypt. Department of Veterinary Population Medicine, University of Minnesota, St. Paul, MN, 55108, USA.Department of Animal Medicine, Faculty of Veterinary Medicine, Zagazig University, Zagazig, Sharkia Province, 44511, Egypt. yasser.mahmmod@irta.cat. Centre de Recerca en Sanitat Animal (CReSA, IRTA), Campus de la Universitat Autònoma de Barcelona, Bellaterra, Cerdanyola del Vallès, 08193, Barcelona, Spain. yasser.mahmmod@irta.cat. Universitat Autònoma de Barcelona, Bellaterra, Cerdanyola del Vallès, 08193, Barcelona, Spain. yasser.mahmmod@irta.cat.Department of Surgery, Anesthesiology, and Radiology, Faculty of Veterinary Medicine, Zagazig University, Zagazig, Sharkia Province, 44511, Egypt.Department of Animal Wealth Development, Faculty of Veterinary Medicine, Zagazig University, Zagazig, Sharkia Province, 44511, Egypt.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31520339

Citation

Sobhy, Nader Maher, et al. "Molecular Detection of Treponema Species Organisms in Foremilk and Udder Cleft Skin of Dairy Cows With Digital Dermatitis." Tropical Animal Health and Production, vol. 52, no. 2, 2020, pp. 815-821.
Sobhy NM, Mahmmod YS, Refaai W, et al. Molecular detection of Treponema species organisms in foremilk and udder cleft skin of dairy cows with digital dermatitis. Trop Anim Health Prod. 2020;52(2):815-821.
Sobhy, N. M., Mahmmod, Y. S., Refaai, W., & Awad, A. (2020). Molecular detection of Treponema species organisms in foremilk and udder cleft skin of dairy cows with digital dermatitis. Tropical Animal Health and Production, 52(2), 815-821. https://doi.org/10.1007/s11250-019-02072-0
Sobhy NM, et al. Molecular Detection of Treponema Species Organisms in Foremilk and Udder Cleft Skin of Dairy Cows With Digital Dermatitis. Trop Anim Health Prod. 2020;52(2):815-821. PubMed PMID: 31520339.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Molecular detection of Treponema species organisms in foremilk and udder cleft skin of dairy cows with digital dermatitis. AU - Sobhy,Nader Maher, AU - Mahmmod,Yasser S, AU - Refaai,Walid, AU - Awad,Ashraf, Y1 - 2019/09/13/ PY - 2019/04/07/received PY - 2019/09/06/accepted PY - 2019/9/15/pubmed PY - 2020/8/1/medline PY - 2019/9/15/entrez KW - Dairy cows KW - Digital dermatitis KW - Reservoirs KW - Treponema KW - Udder cleft SP - 815 EP - 821 JF - Tropical animal health and production JO - Trop Anim Health Prod VL - 52 IS - 2 N2 - Identification of reservoirs and transmission routes of digital dermatitis (DD)-associated Treponema spp. is considered an effective means for controlling DD infection in dairy cows. The objective of this study is to identify and characterize the potential reservoir niches for DD-associated Treponema spp. from healthy udder cleft skin and foremilk in lactating dairy cows. A large dairy farm was visited weekly from March to July 2015. Clinical investigation revealed that a total of 25 lame cows had DD lesions located at the plantar aspect of the interdigital cleft. A total of 75 samples, three per cow, were collected including deep swabs from DD lesions (n = 25), non-aseptically collected foremilk samples (n = 25) and skin swabs from udder cleft (n = 25). Treponema spp. were identified using nested PCR assays and confirmed by DNA sequencing. Results revealed that Treponema phagedenis (T. phagedenis)-like was the most identified species in the foremilk 40% (10/25), in comparison with DD lesions and udder cleft skin samples with 32% (8/25) and 20% (5/25), respectively. On the other hand, Treponema pedis (T. pedis) was the most identified species in the udder cleft skin 80% (20/25), in comparison with DD lesions and foremilk samples with 68% (17/25) and 60% (15/25), respectively. None of the examined samples were identified by PCR as containing DNA from Treponema medium (T. medium) or Treponema vincentii (T. vincentii)-like. To the best of our knowledge, this is the first report for detection of T. phagedenis-like and T. pedis from healthy skin of udder cleft and foremilk samples. Detection of DD Treponema spp. from udder cleft skin and foremilk samples indicates that these sites could be potential reservoirs for spirochetes involved in DD. Udder cleft skin and foremilk may have a role in transmission routes of DD Treponema in dairy farms. SN - 1573-7438 UR - https://www.unboundmedicine.com/medline/citation/31520339/Molecular_detection_of_Treponema_species_organisms_in_foremilk_and_udder_cleft_skin_of_dairy_cows_with_digital_dermatitis_ L2 - https://dx.doi.org/10.1007/s11250-019-02072-0 DB - PRIME DP - Unbound Medicine ER -