Tags

Type your tag names separated by a space and hit enter

Phytochemical, in-vitro biological and chemo-preventive profiling of Arisaema jacquemontii Blume tuber extracts.
BMC Complement Altern Med. 2019 Sep 14; 19(1):256.BC

Abstract

BACKGROUND

Arisaema jacquemontii is traditionally used in treatment of different diseases. In this study, phytochemical, in vitro biological and chemo-preventive screening of A. jacquemontii was carried out to explore its pharmacological potential.

METHODS

The dried tuber of A. jacquemontii was extracted in 11 organic solvent mixture of different polarity. The extracts were screened for phytochemical assays (phenolics and flavonoids), antioxidants potential (free radical scavenging activity, total antioxidant activity, reducing power), biological activities (antibacterial, antifungal, cytotoxic, antileishmanial, protein kinase inhibition), and chemopreventive activities using different cell lines through standard protocols.

RESULTS

Significant amount phenolic contents were determined in EtOH and MeOH extracts (210.3 ± 3.05 and 193.2 ± 3.15 μg GAE/mg, respectively). Maximum flavonoid content was determined in MeOH extract (22.4 ± 4.04 μg QE/mg). Noteworthy, DPPH scavenging activity was also recorded for MeOH extract (87.66%) followed by MeOH+EtOAc extract (85.11%). Considerable antioxidant capacity (7.8 ± 0.12 μg AAE/mg) and reducing power (3.1 ± 0.15 μg AAE/mg) was observed in extract of MeOH. The LC50 against brine shrimp and leishmanial parasite was found 9.01 and 12.87 μg/mL for n-Hex and CHCl3 extracts, respectively. The highest zone of inhibition against Streptomyces hyphae formation (12.5 ± 1.77 mm) by n-Hex extract. Growth zone of inhibition 13.8 ± 1.08 mm was recorded for EtOAc and MeOH extracts, respectively against Micrococcus luteus while 10.0 ± 0.11 mm for MeOH extract against Aspergillus flavus. In-vitro cytotoxic assay showed that n-Hex extract had higher cytotoxicity against DU-145 prostate cancer and HL-60 cancer cell lines. NF-kB and MTP potential showed 34.01 and 44.87 μg/mL for n-Hex and CHCl3 extracts, respectively in chemo-preventive potential.

CONCLUSION

The study concludes that Arisaema jacquemontii bears significant phytochemical activity and pharmacological activities, this plant can be further explored for isolation of active component against a number of aliments.

Authors+Show Affiliations

NUTECH School of Applied Sciences and Humanities, National University of Technology (NUTECH), Islamabad, Pakistan. sairatabassum@nutech.edu.pk.Department of Biotechnology, Quaid-I-Azam University, Islamabad, 45320, Pakistan.Division of Pharmacy Practice Science and Division of Medicinal Chemistry and Pharmacognosy, College of Pharmacy, The Ohio State University, Columbus, OH, USA.Department of Biotechnology, Quaid-I-Azam University, Islamabad, 45320, Pakistan.NUTECH School of Applied Sciences and Humanities, National University of Technology (NUTECH), Islamabad, Pakistan.NUTECH School of Applied Sciences and Humanities, National University of Technology (NUTECH), Islamabad, Pakistan.NUTECH School of Applied Sciences and Humanities, National University of Technology (NUTECH), Islamabad, Pakistan.NUTECH School of Applied Sciences and Humanities, National University of Technology (NUTECH), Islamabad, Pakistan.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31521162

Citation

Tabassum, Saira, et al. "Phytochemical, In-vitro Biological and Chemo-preventive Profiling of Arisaema Jacquemontii Blume Tuber Extracts." BMC Complementary and Alternative Medicine, vol. 19, no. 1, 2019, p. 256.
Tabassum S, Zia M, Carcahe de Blanco EJ, et al. Phytochemical, in-vitro biological and chemo-preventive profiling of Arisaema jacquemontii Blume tuber extracts. BMC Complement Altern Med. 2019;19(1):256.
Tabassum, S., Zia, M., Carcahe de Blanco, E. J., Batool, R., Aslam, R., Hussain, S., Wali, Q., & Gulzar, M. M. (2019). Phytochemical, in-vitro biological and chemo-preventive profiling of Arisaema jacquemontii Blume tuber extracts. BMC Complementary and Alternative Medicine, 19(1), 256. https://doi.org/10.1186/s12906-019-2668-4
Tabassum S, et al. Phytochemical, In-vitro Biological and Chemo-preventive Profiling of Arisaema Jacquemontii Blume Tuber Extracts. BMC Complement Altern Med. 2019 Sep 14;19(1):256. PubMed PMID: 31521162.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Phytochemical, in-vitro biological and chemo-preventive profiling of Arisaema jacquemontii Blume tuber extracts. AU - Tabassum,Saira, AU - Zia,Muhammad, AU - Carcahe de Blanco,Esperanza J, AU - Batool,Riffat, AU - Aslam,Roohi, AU - Hussain,Sajid, AU - Wali,Qamar, AU - Gulzar,Muhammad Mudassar, Y1 - 2019/09/14/ PY - 2019/03/15/received PY - 2019/08/30/accepted PY - 2019/9/16/entrez PY - 2019/9/16/pubmed PY - 2019/12/31/medline KW - Antimicrobial KW - Antioxidant activities KW - NF-kB pathway KW - Phenolic and flavonoid content KW - SRB cytotoxic assay SP - 256 EP - 256 JF - BMC complementary and alternative medicine JO - BMC Complement Altern Med VL - 19 IS - 1 N2 - BACKGROUND: Arisaema jacquemontii is traditionally used in treatment of different diseases. In this study, phytochemical, in vitro biological and chemo-preventive screening of A. jacquemontii was carried out to explore its pharmacological potential. METHODS: The dried tuber of A. jacquemontii was extracted in 11 organic solvent mixture of different polarity. The extracts were screened for phytochemical assays (phenolics and flavonoids), antioxidants potential (free radical scavenging activity, total antioxidant activity, reducing power), biological activities (antibacterial, antifungal, cytotoxic, antileishmanial, protein kinase inhibition), and chemopreventive activities using different cell lines through standard protocols. RESULTS: Significant amount phenolic contents were determined in EtOH and MeOH extracts (210.3 ± 3.05 and 193.2 ± 3.15 μg GAE/mg, respectively). Maximum flavonoid content was determined in MeOH extract (22.4 ± 4.04 μg QE/mg). Noteworthy, DPPH scavenging activity was also recorded for MeOH extract (87.66%) followed by MeOH+EtOAc extract (85.11%). Considerable antioxidant capacity (7.8 ± 0.12 μg AAE/mg) and reducing power (3.1 ± 0.15 μg AAE/mg) was observed in extract of MeOH. The LC50 against brine shrimp and leishmanial parasite was found 9.01 and 12.87 μg/mL for n-Hex and CHCl3 extracts, respectively. The highest zone of inhibition against Streptomyces hyphae formation (12.5 ± 1.77 mm) by n-Hex extract. Growth zone of inhibition 13.8 ± 1.08 mm was recorded for EtOAc and MeOH extracts, respectively against Micrococcus luteus while 10.0 ± 0.11 mm for MeOH extract against Aspergillus flavus. In-vitro cytotoxic assay showed that n-Hex extract had higher cytotoxicity against DU-145 prostate cancer and HL-60 cancer cell lines. NF-kB and MTP potential showed 34.01 and 44.87 μg/mL for n-Hex and CHCl3 extracts, respectively in chemo-preventive potential. CONCLUSION: The study concludes that Arisaema jacquemontii bears significant phytochemical activity and pharmacological activities, this plant can be further explored for isolation of active component against a number of aliments. SN - 1472-6882 UR - https://www.unboundmedicine.com/medline/citation/31521162/Phytochemical_in_vitro_biological_and_chemo_preventive_profiling_of_Arisaema_jacquemontii_Blume_tuber_extracts_ L2 - https://bmccomplementalternmed.biomedcentral.com/articles/10.1186/s12906-019-2668-4 DB - PRIME DP - Unbound Medicine ER -