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Development of UV spectrophotometry methods for concurrent quantification of amlodipine and celecoxib by manipulation of ratio spectra in pure and pharmaceutical formulation.
PLoS One 2019; 14(9):e0222526Plos

Abstract

Recently, the United States Food and Drug Administration approved a new oral dosage preparation of amlodipine besylate (AML) and celecoxib (CEL) for the management of hypertension and osteoarthritis. However, no simultaneous estimation procedures for these two analytes have been described. Hence, two simple, accurate, and precise ultraviolet spectroscopic procedures that manipulated the ratio spectra were established for concurrent quantification of AML and CEL using ethanol as a solvent. The first method involves determining the peak-to-trough amplitude difference of the ratio spectra of AML and CEL. The second method involves determining the peak amplitude of the ratio first derivative (Δλ 4 nm) spectra of AML and CEL at 334.2 nm and 254.2 nm, correspondingly. Both methods showed linearity in the range of 1-6 μg mL-1 for AML and 5-40 μg mL-1 for CEL with an excellent correlation coefficient (<0.999). The proposed procedures were validated by following the International Conference on Harmonization guidelines for accuracy, precision, selectivity, recovery, and stability studies. It is evident from the low %RSD and %RE that both analytical procedures were found to be accurate and precise, respectively. The percent recovery of AML and CEL from the formulation was found to be 99.79% and 99.34% using the ratio-difference method and 100.13% and 99.70% using the ratio first-derivative method, with a low percent relative standard deviation. Further, the proposed techniques permit concurrent quantification of AML and CEL in different concentration ratios without interference from each other; hence, these techniques can be adopted for regular quality-control studies.

Authors+Show Affiliations

Department of Pharmaceutical Sciences, College of Clinical Pharmacy, King Faisal University, Al Ahsa, KSA.Department of Pharmaceutical Sciences, College of Clinical Pharmacy, King Faisal University, Al Ahsa, KSA. Department of Biotechnology and Food Technology, Durban University of Technology, Durban, South Africa.Department of Pharmaceutical Sciences, College of Clinical Pharmacy, King Faisal University, Al Ahsa, KSA.Department of Pharmaceutical Sciences, College of Clinical Pharmacy, King Faisal University, Al Ahsa, KSA.Department of Pharmaceutical Sciences, College of Clinical Pharmacy, King Faisal University, Al Ahsa, KSA.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31525229

Citation

Attimarad, Mahesh, et al. "Development of UV Spectrophotometry Methods for Concurrent Quantification of Amlodipine and Celecoxib By Manipulation of Ratio Spectra in Pure and Pharmaceutical Formulation." PloS One, vol. 14, no. 9, 2019, pp. e0222526.
Attimarad M, Narayanswamy VK, Aldhubaib BE, et al. Development of UV spectrophotometry methods for concurrent quantification of amlodipine and celecoxib by manipulation of ratio spectra in pure and pharmaceutical formulation. PLoS ONE. 2019;14(9):e0222526.
Attimarad, M., Narayanswamy, V. K., Aldhubaib, B. E., SreeHarsha, N., & Nair, A. B. (2019). Development of UV spectrophotometry methods for concurrent quantification of amlodipine and celecoxib by manipulation of ratio spectra in pure and pharmaceutical formulation. PloS One, 14(9), pp. e0222526. doi:10.1371/journal.pone.0222526.
Attimarad M, et al. Development of UV Spectrophotometry Methods for Concurrent Quantification of Amlodipine and Celecoxib By Manipulation of Ratio Spectra in Pure and Pharmaceutical Formulation. PLoS ONE. 2019;14(9):e0222526. PubMed PMID: 31525229.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Development of UV spectrophotometry methods for concurrent quantification of amlodipine and celecoxib by manipulation of ratio spectra in pure and pharmaceutical formulation. AU - Attimarad,Mahesh, AU - Narayanswamy,Venugopla Katarigatta, AU - Aldhubaib,Bandar Essa, AU - SreeHarsha,Nagaraja, AU - Nair,Anroop Balachandran, Y1 - 2019/09/16/ PY - 2019/06/10/received PY - 2019/09/02/accepted PY - 2019/9/17/entrez PY - 2019/9/17/pubmed PY - 2019/9/17/medline SP - e0222526 EP - e0222526 JF - PloS one JO - PLoS ONE VL - 14 IS - 9 N2 - Recently, the United States Food and Drug Administration approved a new oral dosage preparation of amlodipine besylate (AML) and celecoxib (CEL) for the management of hypertension and osteoarthritis. However, no simultaneous estimation procedures for these two analytes have been described. Hence, two simple, accurate, and precise ultraviolet spectroscopic procedures that manipulated the ratio spectra were established for concurrent quantification of AML and CEL using ethanol as a solvent. The first method involves determining the peak-to-trough amplitude difference of the ratio spectra of AML and CEL. The second method involves determining the peak amplitude of the ratio first derivative (Δλ 4 nm) spectra of AML and CEL at 334.2 nm and 254.2 nm, correspondingly. Both methods showed linearity in the range of 1-6 μg mL-1 for AML and 5-40 μg mL-1 for CEL with an excellent correlation coefficient (<0.999). The proposed procedures were validated by following the International Conference on Harmonization guidelines for accuracy, precision, selectivity, recovery, and stability studies. It is evident from the low %RSD and %RE that both analytical procedures were found to be accurate and precise, respectively. The percent recovery of AML and CEL from the formulation was found to be 99.79% and 99.34% using the ratio-difference method and 100.13% and 99.70% using the ratio first-derivative method, with a low percent relative standard deviation. Further, the proposed techniques permit concurrent quantification of AML and CEL in different concentration ratios without interference from each other; hence, these techniques can be adopted for regular quality-control studies. SN - 1932-6203 UR - https://www.unboundmedicine.com/medline/citation/31525229/Development_of_UV_spectrophotometry_methods_for_concurrent_quantification_of_amlodipine_and_celecoxib_by_manipulation_of_ratio_spectra_in_pure_and_pharmaceutical_formulation L2 - http://dx.plos.org/10.1371/journal.pone.0222526 DB - PRIME DP - Unbound Medicine ER -