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Nitric oxide modulates polyamine homeostasis in sunflower seedling cotyledons under salt stress.
Plant Signal Behav. 2019; 14(11):1667730.PS

Abstract

Free polyamine (PA) titers in plants may be regulated through reversible conjugate formation and/or through modulation of their synthesis, transport and degradation. PA signaling involves the well-acknowledged signaling molecule, nitric oxide (NO), which functions in diverse biological processes. Present investigations demonstrate the influence of salt stress (120 mM NaCl) and exogenous NO donor (250 µM Diethylenetriamine, DETA) on PA homeostasis of 2 d old, etiolated sunflower (Helianthus annuus L.) seedling cotyledons as a long-distance signaling response. Significantly enhanced intracellular spermine (Spm) accumulation was observed in seedling cotyledons under salt stress and in response to NO donor, the increase being more pronounced in seedlings treated with NO, evidently as a result of upregulation of the PA biosynthetic enzymes - arginine decarboxylase (ADC) and S-adenosylmethionine decarboxylase (SAMDC) - as revealed by Western blot and confocal imaging (CLSM). Moreover, salt stress induced the activity of polyamine oxidase (PAO), a PA catabolic enzyme, while NO lowered its activity in salt-stressed seedling cotyledons. NO, thus, appears to assist the seedlings in adapting to salt stress by positively regulating PA homeostasis through regulation of PA distribution between free, conjugated and bound forms, increased accumulation of PA biosynthetic enzymes and lowering the rate of PA catabolism.

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Authors+Show Affiliations

Tailor A
Laboratory of Plant Physiology and Biochemistry, Department of Botany, University of Delhi , Delhi , India.
Tandon R
Laboratory of Plant Physiology and Biochemistry, Department of Botany, University of Delhi , Delhi , India.
Bhatla SC
Laboratory of Plant Physiology and Biochemistry, Department of Botany, University of Delhi , Delhi , India.

MeSH

Adenosylmethionine DecarboxylaseCarboxy-LyasesCotyledonHelianthusNitric OxidePolyaminesSalt StressSeedlingsSodium Chloride

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

31526102

Citation

Tailor, Aditi, et al. "Nitric Oxide Modulates Polyamine Homeostasis in Sunflower Seedling Cotyledons Under Salt Stress." Plant Signaling & Behavior, vol. 14, no. 11, 2019, p. 1667730.
Tailor A, Tandon R, Bhatla SC. Nitric oxide modulates polyamine homeostasis in sunflower seedling cotyledons under salt stress. Plant Signal Behav. 2019;14(11):1667730.
Tailor, A., Tandon, R., & Bhatla, S. C. (2019). Nitric oxide modulates polyamine homeostasis in sunflower seedling cotyledons under salt stress. Plant Signaling & Behavior, 14(11), 1667730. https://doi.org/10.1080/15592324.2019.1667730
Tailor A, Tandon R, Bhatla SC. Nitric Oxide Modulates Polyamine Homeostasis in Sunflower Seedling Cotyledons Under Salt Stress. Plant Signal Behav. 2019;14(11):1667730. PubMed PMID: 31526102.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Nitric oxide modulates polyamine homeostasis in sunflower seedling cotyledons under salt stress. AU - Tailor,Aditi, AU - Tandon,Rajesh, AU - Bhatla,Satish C, Y1 - 2019/09/17/ PY - 2019/9/19/pubmed PY - 2020/7/1/medline PY - 2019/9/19/entrez KW - ADC KW - DAO KW - ODC KW - PAO KW - SAMDC KW - nitric oxide KW - polyamine KW - salt stress KW - spermine SP - 1667730 EP - 1667730 JF - Plant signaling & behavior JO - Plant Signal Behav VL - 14 IS - 11 N2 - Free polyamine (PA) titers in plants may be regulated through reversible conjugate formation and/or through modulation of their synthesis, transport and degradation. PA signaling involves the well-acknowledged signaling molecule, nitric oxide (NO), which functions in diverse biological processes. Present investigations demonstrate the influence of salt stress (120 mM NaCl) and exogenous NO donor (250 µM Diethylenetriamine, DETA) on PA homeostasis of 2 d old, etiolated sunflower (Helianthus annuus L.) seedling cotyledons as a long-distance signaling response. Significantly enhanced intracellular spermine (Spm) accumulation was observed in seedling cotyledons under salt stress and in response to NO donor, the increase being more pronounced in seedlings treated with NO, evidently as a result of upregulation of the PA biosynthetic enzymes - arginine decarboxylase (ADC) and S-adenosylmethionine decarboxylase (SAMDC) - as revealed by Western blot and confocal imaging (CLSM). Moreover, salt stress induced the activity of polyamine oxidase (PAO), a PA catabolic enzyme, while NO lowered its activity in salt-stressed seedling cotyledons. NO, thus, appears to assist the seedlings in adapting to salt stress by positively regulating PA homeostasis through regulation of PA distribution between free, conjugated and bound forms, increased accumulation of PA biosynthetic enzymes and lowering the rate of PA catabolism. SN - 1559-2324 UR - https://www.unboundmedicine.com/medline/citation/31526102/Nitric_oxide_modulates_polyamine_homeostasis_in_sunflower_seedling_cotyledons_under_salt_stress_ DB - PRIME DP - Unbound Medicine ER -
Grapherence [↓12 ↑34]

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