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The role of sugar-backbone heterogeneity and chimeras in the simultaneous emergence of RNA and DNA.
Nat Chem 2019NC

Abstract

Hypotheses of the origins of RNA and DNA are generally centred on the prebiotic synthesis of a pristine system (pre-RNA or RNA), which gives rise to its descendent. However, a lack of specificity in the synthesis of genetic polymers would probably result in chimeric sequences; the roles and fate of such sequences are unknown. Here, we show that chimeras, exemplified by mixed threose nucleic acid (TNA)-RNA and RNA-DNA oligonucleotides, preferentially bind to, and act as templates for, homogeneous TNA, RNA and DNA ligands. The chimeric templates can act as a catalyst that mediates the ligation of oligomers to give homogeneous backbone sequences, and the regeneration of the chimeric templates potentiates a scenario for a possible cross-catalytic cycle with amplification. This process provides a proof-of-principle demonstration of a heterogeneity-to-homogeneity scenario and also gives credence to the idea that DNA could appear concurrently with RNA, instead of being its later descendent.

Authors+Show Affiliations

The Department of Chemistry, The Scripps Research Institute, La Jolla, CA, USA.The Department of Chemistry, The Scripps Research Institute, La Jolla, CA, USA. rkrishna@scripps.edu.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31527850

Citation

Bhowmik, Subhendu, and Ramanarayanan Krishnamurthy. "The Role of Sugar-backbone Heterogeneity and Chimeras in the Simultaneous Emergence of RNA and DNA." Nature Chemistry, 2019.
Bhowmik S, Krishnamurthy R. The role of sugar-backbone heterogeneity and chimeras in the simultaneous emergence of RNA and DNA. Nat Chem. 2019.
Bhowmik, S., & Krishnamurthy, R. (2019). The role of sugar-backbone heterogeneity and chimeras in the simultaneous emergence of RNA and DNA. Nature Chemistry, doi:10.1038/s41557-019-0322-x.
Bhowmik S, Krishnamurthy R. The Role of Sugar-backbone Heterogeneity and Chimeras in the Simultaneous Emergence of RNA and DNA. Nat Chem. 2019 Sep 16; PubMed PMID: 31527850.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The role of sugar-backbone heterogeneity and chimeras in the simultaneous emergence of RNA and DNA. AU - Bhowmik,Subhendu, AU - Krishnamurthy,Ramanarayanan, Y1 - 2019/09/16/ PY - 2019/01/10/received PY - 2019/07/31/accepted PY - 2019/9/19/entrez JF - Nature chemistry JO - Nat Chem N2 - Hypotheses of the origins of RNA and DNA are generally centred on the prebiotic synthesis of a pristine system (pre-RNA or RNA), which gives rise to its descendent. However, a lack of specificity in the synthesis of genetic polymers would probably result in chimeric sequences; the roles and fate of such sequences are unknown. Here, we show that chimeras, exemplified by mixed threose nucleic acid (TNA)-RNA and RNA-DNA oligonucleotides, preferentially bind to, and act as templates for, homogeneous TNA, RNA and DNA ligands. The chimeric templates can act as a catalyst that mediates the ligation of oligomers to give homogeneous backbone sequences, and the regeneration of the chimeric templates potentiates a scenario for a possible cross-catalytic cycle with amplification. This process provides a proof-of-principle demonstration of a heterogeneity-to-homogeneity scenario and also gives credence to the idea that DNA could appear concurrently with RNA, instead of being its later descendent. SN - 1755-4349 UR - https://www.unboundmedicine.com/medline/citation/31527850/The_role_of_sugar-backbone_heterogeneity_and_chimeras_in_the_simultaneous_emergence_of_RNA_and_DNA L2 - http://dx.doi.org/10.1038/s41557-019-0322-x DB - PRIME DP - Unbound Medicine ER -