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Highly Sensitive in Vitro Diagnostic System of Pandemic Influenza A (H1N1) Virus Infection with Specific MicroRNA as a Biomarker.
ACS Omega 2019; 4(11):14560-14568AO

Abstract

Several microRNAs (miRNAs) have been reported to be closely related to influenza A virus infection, replication, and immune response. Therefore, the development of the infectious-disease detection system using miRNAs as biomarkers is actively underway. Herein, we identified two miRNAs (miR-181c-5p and miR-1254) as biomarkers for detection of pandemic influenza A H1N1 virus infection and proposed the catalytic hairpin assembly-based in vitro diagnostic (CIVD) system for a highly sensitive diagnosis; this system is composed of two sets of cascade hairpin probes enabling to detect miR-181c-5p and miR-1254. We demonstrated that CIVD kits could not only detect subnanomolar levels of target miRNAs but also distinguish even single-base mismatches. Moreover, this CIVD kit has shown excellent detection performance in real intracellular RNA samples and confirmed results similar to those of conventional methods (microarray and quantitative real-time polymerase chain reaction).

Authors+Show Affiliations

BioNanotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), 125 Gwahak-ro, Yuseong-gu, Daejeon 34141, Republic of Korea. Department of Nanobiotechnology, KRIBB School of Biotechnology, University of Science and Technology (UST), 217 Gajeong-ro, Yuseong-gu, Daejeon 34113, Republic of Korea.BioNanotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), 125 Gwahak-ro, Yuseong-gu, Daejeon 34141, Republic of Korea.BioNanotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), 125 Gwahak-ro, Yuseong-gu, Daejeon 34141, Republic of Korea. Department of Nanobiotechnology, KRIBB School of Biotechnology, University of Science and Technology (UST), 217 Gajeong-ro, Yuseong-gu, Daejeon 34113, Republic of Korea.BioNanotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), 125 Gwahak-ro, Yuseong-gu, Daejeon 34141, Republic of Korea. Department of Nanobiotechnology, KRIBB School of Biotechnology, University of Science and Technology (UST), 217 Gajeong-ro, Yuseong-gu, Daejeon 34113, Republic of Korea.BioNano Health Guard Research Center, 125 Gwahak-ro, Yuseong-gu, Daejeon, 34141, Republic of Korea.BioNanotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), 125 Gwahak-ro, Yuseong-gu, Daejeon 34141, Republic of Korea. Department of Nanobiotechnology, KRIBB School of Biotechnology, University of Science and Technology (UST), 217 Gajeong-ro, Yuseong-gu, Daejeon 34113, Republic of Korea.BioNanotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), 125 Gwahak-ro, Yuseong-gu, Daejeon 34141, Republic of Korea. Department of Nanobiotechnology, KRIBB School of Biotechnology, University of Science and Technology (UST), 217 Gajeong-ro, Yuseong-gu, Daejeon 34113, Republic of Korea.BioNanotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), 125 Gwahak-ro, Yuseong-gu, Daejeon 34141, Republic of Korea. Department of Nanobiotechnology, KRIBB School of Biotechnology, University of Science and Technology (UST), 217 Gajeong-ro, Yuseong-gu, Daejeon 34113, Republic of Korea.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31528810

Citation

Lim, Jaewoo, et al. "Highly Sensitive in Vitro Diagnostic System of Pandemic Influenza a (H1N1) Virus Infection With Specific MicroRNA as a Biomarker." ACS Omega, vol. 4, no. 11, 2019, pp. 14560-14568.
Lim J, Byun J, Guk K, et al. Highly Sensitive in Vitro Diagnostic System of Pandemic Influenza A (H1N1) Virus Infection with Specific MicroRNA as a Biomarker. ACS Omega. 2019;4(11):14560-14568.
Lim, J., Byun, J., Guk, K., Hwang, S. G., Bae, P. K., Jung, J., ... Lim, E. K. (2019). Highly Sensitive in Vitro Diagnostic System of Pandemic Influenza A (H1N1) Virus Infection with Specific MicroRNA as a Biomarker. ACS Omega, 4(11), pp. 14560-14568. doi:10.1021/acsomega.9b01790.
Lim J, et al. Highly Sensitive in Vitro Diagnostic System of Pandemic Influenza a (H1N1) Virus Infection With Specific MicroRNA as a Biomarker. ACS Omega. 2019 Sep 10;4(11):14560-14568. PubMed PMID: 31528810.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Highly Sensitive in Vitro Diagnostic System of Pandemic Influenza A (H1N1) Virus Infection with Specific MicroRNA as a Biomarker. AU - Lim,Jaewoo, AU - Byun,Jihyun, AU - Guk,Kyeonghye, AU - Hwang,Seul Gee, AU - Bae,Pan Kee, AU - Jung,Juyeon, AU - Kang,Taejoon, AU - Lim,Eun-Kyung, Y1 - 2019/08/26/ PY - 2019/06/17/received PY - 2019/07/31/accepted PY - 2019/9/19/entrez PY - 2019/9/19/pubmed PY - 2019/9/19/medline SP - 14560 EP - 14568 JF - ACS omega JO - ACS Omega VL - 4 IS - 11 N2 - Several microRNAs (miRNAs) have been reported to be closely related to influenza A virus infection, replication, and immune response. Therefore, the development of the infectious-disease detection system using miRNAs as biomarkers is actively underway. Herein, we identified two miRNAs (miR-181c-5p and miR-1254) as biomarkers for detection of pandemic influenza A H1N1 virus infection and proposed the catalytic hairpin assembly-based in vitro diagnostic (CIVD) system for a highly sensitive diagnosis; this system is composed of two sets of cascade hairpin probes enabling to detect miR-181c-5p and miR-1254. We demonstrated that CIVD kits could not only detect subnanomolar levels of target miRNAs but also distinguish even single-base mismatches. Moreover, this CIVD kit has shown excellent detection performance in real intracellular RNA samples and confirmed results similar to those of conventional methods (microarray and quantitative real-time polymerase chain reaction). SN - 2470-1343 UR - https://www.unboundmedicine.com/medline/citation/31528810/Highly_Sensitive_in_Vitro_Diagnostic_System_of_Pandemic_Influenza_A_(H1N1)_Virus_Infection_with_Specific_MicroRNA_as_a_Biomarker L2 - https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/31528810/ DB - PRIME DP - Unbound Medicine ER -