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Identification of the quantitative trait loci controlling spike-related traits in hexaploid wheat (Triticum aestivum L.).
Planta. 2019 Dec; 250(6):1967-1981.P

Abstract

MAIN CONCLUSION

Totally, 48 loci responsible for six spike-related traits were identified in wheat, and a major locus QGl-4A for grain length was mapped and validated for marker-assisted selection in breeding. Wheat yield is determined by the number of spikes, number of grains per spike (GN), and one-thousand kernel weight (TKW), among which GN and TKW are greatly related to the spike development and thus the spike-related traits, including spike length (SL), number of spikelet per spike (SN), grain length (GL) and grain width (GW). To identify the key loci governing the spike-related traits (SL, SN, GN, TKW, GL and GW), we conducted the quantitative trait loci (QTL) analysis combined with wheat 660K SNP chip and Kompetitive allele-specific PCR (KASP) assay, using the F2 and F2:3 populations derived from Luohan6 (LH6) with big spike and grain and Zhengmai366 with small spike and grain, and identified a total of 48 QTLs on 18 chromosomes. Moreover, a major stable QTL for GL on chromosome 4A, designated as QGl-4A, was mapped into a 0.37 cM interval between KASP markers Xib4A-10 and Xib4A-12, corresponding to 20 Mb physical region in the Chinese Spring genome. This QTL explained 17.30% and 5.12% of the phenotypic variation for GL in the F2 and F2:3 populations. Further association analysis of flanking markers Xib4A-10 and Xib4A-12 in 192 wheat varieties showed that these two markers could be used for marker-assisted selection in breeding. These results provide valuable information for map-based cloning of the target genes involved in the regulation of spike-related traits in common wheat.

Authors+Show Affiliations

Key Laboratory of Plant Molecular Physiology, CAS Center for Excellence in Molecular Plant Sciences, Institute of Botany, Chinese Academy of Sciences, Beijing, 100093, China.Key Laboratory of Plant Molecular Physiology, CAS Center for Excellence in Molecular Plant Sciences, Institute of Botany, Chinese Academy of Sciences, Beijing, 100093, China. University of Chinese Academy of Sciences, Beijing, 100049, China.Key Laboratory of Plant Molecular Physiology, CAS Center for Excellence in Molecular Plant Sciences, Institute of Botany, Chinese Academy of Sciences, Beijing, 100093, China. University of Chinese Academy of Sciences, Beijing, 100049, China.Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, 610041, China.Key Laboratory of Plant Molecular Physiology, CAS Center for Excellence in Molecular Plant Sciences, Institute of Botany, Chinese Academy of Sciences, Beijing, 100093, China.Henan Science and Technology University, Luoyang, 471023, Henan, China.Key Laboratory of Plant Molecular Physiology, CAS Center for Excellence in Molecular Plant Sciences, Institute of Botany, Chinese Academy of Sciences, Beijing, 100093, China. huyuxin@ibcas.ac.cn. National Center for Plant Gene Research, Beijing, 100093, China. huyuxin@ibcas.ac.cn.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31529397

Citation

Cao, Pei, et al. "Identification of the Quantitative Trait Loci Controlling Spike-related Traits in Hexaploid Wheat (Triticum Aestivum L.)." Planta, vol. 250, no. 6, 2019, pp. 1967-1981.
Cao P, Liang X, Zhao H, et al. Identification of the quantitative trait loci controlling spike-related traits in hexaploid wheat (Triticum aestivum L.). Planta. 2019;250(6):1967-1981.
Cao, P., Liang, X., Zhao, H., Feng, B., Xu, E., Wang, L., & Hu, Y. (2019). Identification of the quantitative trait loci controlling spike-related traits in hexaploid wheat (Triticum aestivum L.). Planta, 250(6), 1967-1981. https://doi.org/10.1007/s00425-019-03278-0
Cao P, et al. Identification of the Quantitative Trait Loci Controlling Spike-related Traits in Hexaploid Wheat (Triticum Aestivum L.). Planta. 2019;250(6):1967-1981. PubMed PMID: 31529397.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Identification of the quantitative trait loci controlling spike-related traits in hexaploid wheat (Triticum aestivum L.). AU - Cao,Pei, AU - Liang,Xiaona, AU - Zhao,Hong, AU - Feng,Bo, AU - Xu,Enjun, AU - Wang,Liming, AU - Hu,Yuxin, Y1 - 2019/09/16/ PY - 2019/04/28/received PY - 2019/09/08/accepted PY - 2019/9/19/pubmed PY - 2020/3/10/medline PY - 2019/9/19/entrez KW - Grain length KW - Grain weight KW - KASP markers KW - QTL KW - Spike-related traits KW - Wheat SP - 1967 EP - 1981 JF - Planta JO - Planta VL - 250 IS - 6 N2 - MAIN CONCLUSION: Totally, 48 loci responsible for six spike-related traits were identified in wheat, and a major locus QGl-4A for grain length was mapped and validated for marker-assisted selection in breeding. Wheat yield is determined by the number of spikes, number of grains per spike (GN), and one-thousand kernel weight (TKW), among which GN and TKW are greatly related to the spike development and thus the spike-related traits, including spike length (SL), number of spikelet per spike (SN), grain length (GL) and grain width (GW). To identify the key loci governing the spike-related traits (SL, SN, GN, TKW, GL and GW), we conducted the quantitative trait loci (QTL) analysis combined with wheat 660K SNP chip and Kompetitive allele-specific PCR (KASP) assay, using the F2 and F2:3 populations derived from Luohan6 (LH6) with big spike and grain and Zhengmai366 with small spike and grain, and identified a total of 48 QTLs on 18 chromosomes. Moreover, a major stable QTL for GL on chromosome 4A, designated as QGl-4A, was mapped into a 0.37 cM interval between KASP markers Xib4A-10 and Xib4A-12, corresponding to 20 Mb physical region in the Chinese Spring genome. This QTL explained 17.30% and 5.12% of the phenotypic variation for GL in the F2 and F2:3 populations. Further association analysis of flanking markers Xib4A-10 and Xib4A-12 in 192 wheat varieties showed that these two markers could be used for marker-assisted selection in breeding. These results provide valuable information for map-based cloning of the target genes involved in the regulation of spike-related traits in common wheat. SN - 1432-2048 UR - https://www.unboundmedicine.com/medline/citation/31529397/Identification_of_the_quantitative_trait_loci_controlling_spike_related_traits_in_hexaploid_wheat__Triticum_aestivum_L___ L2 - https://dx.doi.org/10.1007/s00425-019-03278-0 DB - PRIME DP - Unbound Medicine ER -