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Specialist and Generalist Fungal Parasites Induce Distinct Biochemical Changes in the Mandible Muscles of Their Host.
Int J Mol Sci. 2019 Sep 17; 20(18)IJ

Abstract

Some parasites have evolved the ability to adaptively manipulate host behavior. One notable example is the fungus Ophiocordyceps unilateralis sensu lato, which has evolved the ability to alter the behavior of ants in ways that enable fungal transmission and lifecycle completion. Because host mandibles are affected by the fungi, we focused on understanding changes in the metabolites of muscles during behavioral modification. We used High-Performance Liquid Chromatography-Mass/Mass (HPLC-MS/MS) to detect the metabolite difference between controls and O. unilateralis-infected ants. There was a significant difference between the global metabolome of O. unilateralis-infected ants and healthy ants, while there was no significant difference between the Beauveria bassiana treatment ants group compared to the healthy ants. A total of 31 and 16 of metabolites were putatively identified from comparisons of healthy ants with O. unilateralis-infected ants and comparisons of B. bassiana with O. unilateralis-infected samples, respectively. This result indicates that the concentrations of sugars, purines, ergothioneine, and hypoxanthine were significantly increased in O. unilateralis-infected ants in comparison to healthy ants and B. bassiana-infected ants. This study provides a comprehensive metabolic approach for understanding the interactions, at the level of host muscles, between healthy ants and fungal parasites.

Authors+Show Affiliations

Key Laboratory of Biopesticide and Chemical Biology, Ministry of Education, Fuzhou 350002, China. zhengshanshan0428@gmail.com. College of Plant Protection College, Fujian Agriculture and Forestry University, Fuzhou 350002, China. zhengshanshan0428@gmail.com. Department of Entomology, Pennsylvania State University, University Park, PA 16802, USA. zhengshanshan0428@gmail.com.Center for Infectious Diseases Dynamics, Pennsylvania State University, University Park, PA 16802, USA. raquelgloreto@gmail.com. CAPES Foundation, Ministry of Education of Brazil, Brasilia 70040-020, DF, Brazil. raquelgloreto@gmail.com.Metabolomics Core Facility, Huck Institutes of Life Sciences, Pennsylvania State University, University Park, PA 16802, USA. pbs13@psu.edu.Center for Molecular Toxicology and Carcinogenesis, Department of Veterinary and Biomedical Sciences, Pennsylvania State University, University Park, PA 16802, USA. adp117@psu.edu.Department of Entomology, Pennsylvania State University, University Park, PA 16802, USA. dph14@psu.edu. Department of Biology, Pennsylvania State University, University Park, PA 16802, USA. dph14@psu.edu.Key Laboratory of Biopesticide and Chemical Biology, Ministry of Education, Fuzhou 350002, China. liande_wang@126.com. College of Plant Protection College, Fujian Agriculture and Forestry University, Fuzhou 350002, China. liande_wang@126.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31533250

Citation

Zheng, Shanshan, et al. "Specialist and Generalist Fungal Parasites Induce Distinct Biochemical Changes in the Mandible Muscles of Their Host." International Journal of Molecular Sciences, vol. 20, no. 18, 2019.
Zheng S, Loreto R, Smith P, et al. Specialist and Generalist Fungal Parasites Induce Distinct Biochemical Changes in the Mandible Muscles of Their Host. Int J Mol Sci. 2019;20(18).
Zheng, S., Loreto, R., Smith, P., Patterson, A., Hughes, D., & Wang, L. (2019). Specialist and Generalist Fungal Parasites Induce Distinct Biochemical Changes in the Mandible Muscles of Their Host. International Journal of Molecular Sciences, 20(18). https://doi.org/10.3390/ijms20184589
Zheng S, et al. Specialist and Generalist Fungal Parasites Induce Distinct Biochemical Changes in the Mandible Muscles of Their Host. Int J Mol Sci. 2019 Sep 17;20(18) PubMed PMID: 31533250.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Specialist and Generalist Fungal Parasites Induce Distinct Biochemical Changes in the Mandible Muscles of Their Host. AU - Zheng,Shanshan, AU - Loreto,Raquel, AU - Smith,Philip, AU - Patterson,Andrew, AU - Hughes,David, AU - Wang,Liande, Y1 - 2019/09/17/ PY - 2019/08/06/received PY - 2019/09/09/revised PY - 2019/09/10/accepted PY - 2019/9/20/entrez PY - 2019/9/20/pubmed PY - 2019/9/20/medline KW - behavioral manipulation KW - fungal pathogens KW - mandible muscle KW - muscular atrophy JF - International journal of molecular sciences JO - Int J Mol Sci VL - 20 IS - 18 N2 - Some parasites have evolved the ability to adaptively manipulate host behavior. One notable example is the fungus Ophiocordyceps unilateralis sensu lato, which has evolved the ability to alter the behavior of ants in ways that enable fungal transmission and lifecycle completion. Because host mandibles are affected by the fungi, we focused on understanding changes in the metabolites of muscles during behavioral modification. We used High-Performance Liquid Chromatography-Mass/Mass (HPLC-MS/MS) to detect the metabolite difference between controls and O. unilateralis-infected ants. There was a significant difference between the global metabolome of O. unilateralis-infected ants and healthy ants, while there was no significant difference between the Beauveria bassiana treatment ants group compared to the healthy ants. A total of 31 and 16 of metabolites were putatively identified from comparisons of healthy ants with O. unilateralis-infected ants and comparisons of B. bassiana with O. unilateralis-infected samples, respectively. This result indicates that the concentrations of sugars, purines, ergothioneine, and hypoxanthine were significantly increased in O. unilateralis-infected ants in comparison to healthy ants and B. bassiana-infected ants. This study provides a comprehensive metabolic approach for understanding the interactions, at the level of host muscles, between healthy ants and fungal parasites. SN - 1422-0067 UR - https://www.unboundmedicine.com/medline/citation/31533250/Specialist_and_Generalist_Fungal_Parasites_Induce_Distinct_Biochemical_Changes_in_the_Mandible_Muscles_of_Their_Host_ L2 - https://www.mdpi.com/resolver?pii=ijms20184589 DB - PRIME DP - Unbound Medicine ER -